Vaccine, Journal Year: 2024, Volume and Issue: 45, P. 126648 - 126648
Published: Dec. 20, 2024
Language: Английский
Pharmacological Research, Journal Year: 2023, Volume and Issue: 198, P. 106999 - 106999
Published: Nov. 19, 2023
Cell-to-cell communication strategies include extracellular vesicles (EVs) in plants and animals. The bioactive molecules a diet rich vegetables fruits are associated with disease-preventive effects. Plant-derived EVs (PDEVs) biogenetically morphologically comparable to mammalian transport molecules, including miRNAs. However, the biological functions of PDEVs not fully understood, standard isolation protocols lacking. Here, were isolated from four foods combination ultracentrifugation size exclusion chromatography, evaluated as vehicles for enhanced synthetic In addition, role food-derived carriers dietary (poly)phenols other secondary metabolites was investigated. broccoli, pomegranate, apple, orange efficiently characterized. all sources, 4 miRNA families present tissues EVs. miRNAs broccoli fruit-derived showed reduced RNase degradation ferried inside exposed cells. transfected ath-miR159a, ath-miR162a-3p, ath-miR166b-3p, ath-miR396b-5p toxic effects on human cells, did natural alone. trace amounts phytochemicals, flavonoids, anthocyanidins, phenolic acids, or glucosinolates. Thus, can act nanocarriers functional that could be used RNA-based therapy.
Language: Английский
Citations
44
Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 394 - 394
Published: March 10, 2025
Most eukaryotic and prokaryotic cells have the potential to secrete a group of structures/membrane-bound organelles, collectively referred as extracellular vesicles (EVs), which offer several advantages producer/receiver cells. This review provides an overview EVs from plant sources with emphasis on their health-promoting possible use therapeutic agents. highlights essential biological effects plant-derived vesicles, including immune modulation, anticancer activities, protection against chemical toxicity pathogens, well anti-aging, anti-melanogenesis, anti-arthritic effects, along ongoing clinical studies. Evidence revealed that EVs’ contents exert beneficial properties through regulating important signaling pathways by transferring miRNAs other components. Taken all together, data proposed can be utilized nutritional compounds agents, such drug carriers. However, this emerging research area requires further in vitro/in vivo studies trials determine exact underlying mechanisms positive health treating various diseases.
Language: Английский
Citations
2
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 1154 - 1154
Published: Jan. 18, 2024
Dramatic shifts in global climate have intensified abiotic and biotic stress faced by plants. Plant microRNAs (miRNAs)—20–24 nucleotide non-coding RNA molecules—form a key regulatory system of plant gene expression; playing crucial roles growth; development; defense against stress. Moreover, they participate cross-kingdom communication. This communication encompasses interactions with other plants, microorganisms, insect species, collectively exerting profound influence on the agronomic traits crops. article comprehensively reviews biosynthesis miRNAs explores their impact growth, development, resistance through endogenous, non-transboundary mechanisms. Furthermore, this review delves into effects pests. It proceeds to specifically discuss design modification strategies for artificial (amiRNAs), as well protection transport exosome-like nanovesicles (ELNVs), expanding potential applications crop breeding. Finally, current limitations associated harnessing are addressed, utilization synthetic biology is proposed facilitate heterologous expression large-scale production miRNAs. novel approach suggests plant-based solution address future biosafety concerns agriculture.
Language: Английский
Citations
8
Vaccines, Journal Year: 2024, Volume and Issue: 12(2), P. 200 - 200
Published: Feb. 15, 2024
Nucleic acid delivery through extracellular vesicles (EVs) is a well-preserved evolutionary mechanism in all life kingdoms including eukaryotes, prokaryotes, and plants. EVs naturally allow horizontal transfer of native as well exogenous functional mRNAs, which once incorporated are protected from enzymatic degradation. This observation has prompted researchers to investigate whether different sources, plants, could be used for vaccine delivery. Several studies using human or bacterial expressing mRNA recombinant SARS-CoV-2 proteins showed induction humoral cell mediated immune response. Moreover, EV-based vaccines presenting the natural configuration viral antigens have demonstrated advantages conferring long-lasting immunization lower toxicity than synthetic nanoparticles. Edible plant-derived were shown an alternative delivery, especially via oral administration. obtained orange juice (oEVs) loaded with mRNAs their cargo degradation, stable at room temperature one year, able trigger response mice. Lyophilized oEVs containing S1 administered rats gavage induced specific generation blocking antibodies, IgA Th1 lymphocyte activation. In conclusion, mRNA-containing developing new due optimal mucosal absorption, resistance stress conditions, ability stimulate cellular
Language: Английский
Citations
8
Vaccines, Journal Year: 2025, Volume and Issue: 13(2), P. 191 - 191
Published: Feb. 15, 2025
Using plants as bioreactors, molecular farming has emerged a versatile and sustainable platform for producing recombinant vaccines, therapeutic proteins, industrial enzymes, nutraceuticals. This innovative approach leverages the unique advantages of plants, including scalability, cost-effectiveness, reduced risk contamination with human pathogens. Recent advancements in gene editing, transient expression systems, nanoparticle-based delivery technologies have significantly enhanced efficiency versatility plant-based systems. Particularly vaccine development, demonstrated its potential notable successes such Medicago's Covifenz COVID-19, illustrating capacity platforms to address global health emergencies rapidly. Furthermore, edible vaccines opened new avenues mainly settings low resources where cold chain used conventional logistics is challenge. However, optimization protein yield stability, complexity purification processes, regulatory hurdles are some challenges that still remain. review discusses current status development using operational mechanisms plant platforms, major applications prevention infectious diseases, developments, nanoparticle-mediated cancer vaccines. The discussion will also touch on ethical considerations, framework, future trends respect transformative plant-derived ensuring greater accessibility cost-effectiveness vaccination. field holds great promise disease area and, indeed, personalized medicine biopharmaceuticals near future.
Language: Английский
Citations
1
Published: Jan. 10, 2024
Nucleic acid delivery through extracellular vesicles (EVs) is a well-preserved evolutionary mechanism in all life kingdoms including eukaryotes, prokaryotes, and plants. EVs naturally allow horizontal transfer of native as well exogenous functional mRNA, that once incorporated protected from enzymatic degradation. The possible use carriers for vaccine has been investigated showing expressing recombinant SARS-CoV-2 S protein induced an immune response. Moreover, EV-based vaccines presenting the natural configuration viral antigens have advantages conferring long lasting immunization lower toxicity than synthetic nanoparticles. To overcome manufacturing limitations derived eukaryotic cells, edible plant-derived studied attractive alternative delivery, especially mucosal administration. We recently showed obtained orange juice (oEVs) loaded with mRNAs their cargo degradation were stable at room temperature one year. Lyophilized oEVs containing S1 mRNA administered to rats by gavage they specific humoral response generation blocking antibodies, IgA, are first barrier adaptive Th1 cytokine secretion occurred, suggesting lymphocyte activation. In conclusion, mRNA-containing could be used developing new oral due optimal absorption, resistance stress conditions, ability stimulate cellular
Language: Английский
Citations
7
Discover Oncology, Journal Year: 2024, Volume and Issue: 15(1)
Published: April 29, 2024
Abstract The use of cancer immunotherapeutics is currently increasing. Cancer vaccines, as a form immunotherapy, are gaining much attention in the medical community since specific tumor-antigens can activate immune cells to induce an anti-tumor response. However, delivery vaccines presents many issues for research scientists when designing treatments and requires further investigation. Nanoparticles, synthetic liposomes, bacterial vectors, viral particles, mammalian exosomes have delivered vaccines. In contrast, these nanotechnologies produces cytotoxicity, immunogenicity, rapid clearance by mononuclear phagocyte system (MPS). Plant-exosome-like nanovesicles (PELNVs) provide solutions challenges because they innocuous nonimmunogenic delivering nanomedicines. Hence, this review will describe potential PELNVs deliver review, different approaches vaccine be detailed, mechanism oral vaccination described, discuss improved drug systems via administration while also addressing subsequent advancing their usage into clinical setting.
Language: Английский
Citations
7
Biomaterials Research, Journal Year: 2024, Volume and Issue: 28
Published: Jan. 1, 2024
Stem-cell-derived extracellular vesicles (EVs) are emerging as an alternative approach to stem cell therapy. Successful lyophilization of EVs could enable convenient storage and distribution EV medicinal products at room temperature for long periods, thus considerably increasing the accessibility therapeutics patients. In this study, we aimed identify appropriate lyoprotectant composition reconstitution stem-cell-derived EVs. MSC-derived were lyophilized using different lyoprotectants, such dimethyl sulfoxide, mannitol, trehalose, sucrose, varying concentrations. Our results revealed that a mixture trehalose sucrose high concentrations support formation amorphous ice by enriching phase solution, which successfully inhibited acceleration buffer component crystallization during lyophilization. Lyophilized reconstituted thoroughly evaluated concentration size, morphology, protein RNA content. The therapeutic effects examined tube assay with human umbilical vein endothelial cells. After rehydration EVs, most their generic characteristics well-maintained, capacity recovered levels similar those freshly collected morphologies initial features fresh group until day 30 temperature, although appeared decrease after 7 days. study suggests particularly lyophilization, encourage applications in health industry.
Language: Английский
Citations
5
Biomolecules, Journal Year: 2025, Volume and Issue: 15(3), P. 359 - 359
Published: March 1, 2025
Modernization of existing methods for the delivery mRNA is vital in advanced therapeutics. Traditionally, has faced obstacles poor stability due to enzymatic degradation. This work examines cutting-edge formulation and emerging techniques safer vaccines. Inspired by success lipid nanoparticles (LNP) delivering vaccines COVID-19, a variety other formulations have been developed deliver diverse infections. The meritorious features nanoparticle-based strategies, including LNP, polymeric, dendrimers, polysaccharide-based, peptide-derived, carbon metal-based, DNA nanostructures, hybrid, extracellular vesicles, examined. impact these platforms on vaccine efficacy, protection from degradation, cellular uptake, controlled release, immunogenicity discussed detail. Even with significant developments, there are certain limitations overcome, toxicity concerns, limited information about immune pathways, need maintain cold chain, necessity optimizing administration methods. Continuous innovation essential improving systems Future research directions proposed address challenges expand their potential prophylactic therapeutic application.
Language: Английский
Citations
0
Chemical Reviews, Journal Year: 2025, Volume and Issue: unknown
Published: April 1, 2025
The development of nucleic acid and protein drugs for oral delivery has lagged behind their production conventional nonoral routes. Over the past decade, evolution DNA- RNA-based technologies combined with innovation state-of-the-art vehicles acids brought rapid advancements to biopharmaceutical field. Nucleic therapies have potential achieve long-lasting effects, or even cures, by inhibiting editing genes, which is not possible small-molecule drugs. However, challenges limitations must be addressed before these can provide cures chronic conditions rare diseases, rather than only offering temporary relief. proteins face premature degradation in acidic, enzyme-rich stomach environment are rapidly cleared liver. To overcome challenges, various been developed transport therapeutic compounds intestines, where active released gut microbiota mucosal immune system also play an important role. This review provides a comprehensive overview promises pitfalls associated route administration biologics, current systems, applications orally delivered therapeutics, considerations translation therapeutics into clinical practice.
Language: Английский
Citations
0