Capsaicin reverses cisplatin resistance in tongue squamous cell carcinoma by inhibiting the Warburg effect and facilitating mitochondrial‐dependent apoptosis via the AMPK/AKT/mTOR axis DOI
Xia‐Yang Liu, Li Zhuang,

Qiwei Zhao

et al.

Cell Biology International, Journal Year: 2024, Volume and Issue: 48(8), P. 1097 - 1110

Published: May 5, 2024

Abstract Cisplatin is commonly used for the chemotherapy of tongue squamous cell carcinoma (TSCC); however, adverse side effects and drug resistance impact its therapeutic efficacy. Capsaicin an active ingredient in chili peppers that exerts antitumor effects, whether it on cisplatin‐resistant cells remains unknown. Therefore, this study, we investigated effect capsaicin cisplatin TSCC explored underlying mechanisms. A line was established by treated with increasing concentrations. Combined treatment decreased glucose consumption lactate dehydrogenase activity increased adenosine triphosphate production both vitro vivo, suggesting inhibition Warburg effect. Moreover, combined induced apoptosis significantly upregulated levels proapoptotic proteins, such as Bax, cleaved caspase‐3, ‐7, ‐9, apoptosis‐inducing factor. In contrast, antiapoptotic protein, Bcl‐2, were downregulated. Additionally, LKB1 AMPK activities stimulated, whereas those AKT mTOR suppressed. Notably, knockdown abolished inhibitory AKT/mTOR signaling pathway Overall, reversed inhibiting facilitating mitochondrial‐dependent via AMPK/AKT/mTOR axis. Our findings suggest combination therapy a potentially novel strategy highlight promising adjuvant treatment.

Language: Английский

Capsaicin reverses cisplatin resistance in tongue squamous cell carcinoma by inhibiting the Warburg effect and facilitating mitochondrial‐dependent apoptosis via the AMPK/AKT/mTOR axis DOI
Xia‐Yang Liu, Li Zhuang,

Qiwei Zhao

et al.

Cell Biology International, Journal Year: 2024, Volume and Issue: 48(8), P. 1097 - 1110

Published: May 5, 2024

Abstract Cisplatin is commonly used for the chemotherapy of tongue squamous cell carcinoma (TSCC); however, adverse side effects and drug resistance impact its therapeutic efficacy. Capsaicin an active ingredient in chili peppers that exerts antitumor effects, whether it on cisplatin‐resistant cells remains unknown. Therefore, this study, we investigated effect capsaicin cisplatin TSCC explored underlying mechanisms. A line was established by treated with increasing concentrations. Combined treatment decreased glucose consumption lactate dehydrogenase activity increased adenosine triphosphate production both vitro vivo, suggesting inhibition Warburg effect. Moreover, combined induced apoptosis significantly upregulated levels proapoptotic proteins, such as Bax, cleaved caspase‐3, ‐7, ‐9, apoptosis‐inducing factor. In contrast, antiapoptotic protein, Bcl‐2, were downregulated. Additionally, LKB1 AMPK activities stimulated, whereas those AKT mTOR suppressed. Notably, knockdown abolished inhibitory AKT/mTOR signaling pathway Overall, reversed inhibiting facilitating mitochondrial‐dependent via AMPK/AKT/mTOR axis. Our findings suggest combination therapy a potentially novel strategy highlight promising adjuvant treatment.

Language: Английский

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