Silk Protein Gene Engineering and Its Applications: Recent Advances in Biomedicine Driven by Molecular Biotechnology DOI Creative Commons

Xingxiang Ji,

Yanyan Li, Jingsheng Wang

et al.

Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 599 - 626

Published: Jan. 1, 2025

Silk protein, as a natural polymer material with unique structures and properties, exhibits tremendous potential in the biomedical field. Given limited production restricted properties of silk proteins, molecular biotechnology has been extensively applied protein genetic engineering to produce novel proteins specific properties. This review outlines roles major model organisms, such silkworms spiders, production, provides detailed introduction applications gene editing technologies (eg, CRISPR-Cas9), transgenic expression technologies, synthetic biology techniques engineering. By analyzing factors influencing expression, this further elaborates on innovative drug delivery systems, tissue regenerative medicine skin, bone, cartilage, vascular repair), well antibacterial immune strategies. Notably, modified expressed by demonstrate significant advantages enhancing bioavailability promoting cell proliferation differentiation. In conclusion, engineering, through continuous innovations biotechnology, provided an effective pathway for high-performance materials. The extensive these field have not only expanded functionality but also offered new approaches address medical challenges. future, development will rely interdisciplinary integration promote in-depth research expansion industrial proteins.

Language: Английский

Review of Spider Silk Applications in Biomedical and Tissue Engineering DOI Creative Commons
Marija Branković, Fatima Živić, Nenad Grujović

et al.

Biomimetics, Journal Year: 2024, Volume and Issue: 9(3), P. 169 - 169

Published: March 11, 2024

This review will present the latest research related to production and application of spider silk silk-based materials in reconstructive regenerative medicine tissue engineering, with a focus on musculoskeletal tissues, including skin regeneration repair bone cartilage, ligaments, muscle tissue, peripheral nerves, artificial blood vessels. Natural synthesis is reviewed, further recombinant proteins. Research insights into possible structures, like fibers (1D), coatings (2D), 3D constructs, porous hydrogels, organ-on-chip designs, have been reviewed considering design bioactive for smart medical implants drug delivery systems. Silk one toughest natural materials, high strain at failure mechanical strength. Novel biomaterials fibroin can mimic structure promote new growth. proteins are important designing tissue-on-chip or technologies micro devices precise engineering tissues organs, disease modeling, selection adequate treatments. Recent indicates that (films, capsules, liposomes coated proteins) has potential provide controlled release target destination. However, even clear advantages, there still challenges need research, clinical trials.

Language: Английский

Citations

15
3D-Printed Silk Proteins for Bone Tissue Regeneration and Associated Immunomodulation DOI
Yusuf Olatunji Waidi, Souvik Debnath, Sudipto Datta

et al.

Biomacromolecules, Journal Year: 2024, Volume and Issue: 25(9), P. 5512 - 5540

Published: Aug. 12, 2024

Current bone repair methods have limitations, prompting the exploration of innovative approaches. Tissue engineering emerges as a promising solution, leveraging biomaterials to craft scaffolds replicating natural environment, facilitating cell growth and differentiation. Among fabrication techniques, three-dimensional (3D) printing stands out for its ability tailor intricate scaffolds. Silk proteins (SPs), known their mechanical strength biocompatibility, are an excellent choice 3D-printed tissue (BTE) This article comprehensively reviews biology, 3D printing, unique attributes SPs, specifically detailing criteria scaffold such composition, structure, mechanics, cellular responses. It examines structural, mechanical, biological emphasizing suitability BTE. Recent studies on diverse approaches using SPs-based BTE highlighted, alongside advancements in four-dimensional (4D) role osteo-immunomodulation. Future directions use SPs outlined.

Language: Английский

Citations

4
Silk Protein Gene Engineering and Its Applications: Recent Advances in Biomedicine Driven by Molecular Biotechnology DOI Creative Commons

Xingxiang Ji,

Yanyan Li, Jingsheng Wang

et al.

Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 599 - 626

Published: Jan. 1, 2025

Silk protein, as a natural polymer material with unique structures and properties, exhibits tremendous potential in the biomedical field. Given limited production restricted properties of silk proteins, molecular biotechnology has been extensively applied protein genetic engineering to produce novel proteins specific properties. This review outlines roles major model organisms, such silkworms spiders, production, provides detailed introduction applications gene editing technologies (eg, CRISPR-Cas9), transgenic expression technologies, synthetic biology techniques engineering. By analyzing factors influencing expression, this further elaborates on innovative drug delivery systems, tissue regenerative medicine skin, bone, cartilage, vascular repair), well antibacterial immune strategies. Notably, modified expressed by demonstrate significant advantages enhancing bioavailability promoting cell proliferation differentiation. In conclusion, engineering, through continuous innovations biotechnology, provided an effective pathway for high-performance materials. The extensive these field have not only expanded functionality but also offered new approaches address medical challenges. future, development will rely interdisciplinary integration promote in-depth research expansion industrial proteins.

Language: Английский

Citations

0