Heat Stress Impairs Endometrial Function During Implantation by Regulating Autophagy in Hainan Black Goat DOI Creative Commons
Xiaoping Li,

Yanyu Sun,

Min Yi

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(22), P. 3213 - 3213

Published: Nov. 8, 2024

Heat stress (HS) is a significant factor that adversely affects the health, welfare, and productivity of domestic animals, particularly impacting embryo implantation rates. However, effects HS on endometrial function during peri-implantation period in Hainan black goats remain unclear. This study explores influence endometrium these goats. We collected uterine tissue samples from both control heat-stressed performed vitro experiments using 2 × factorial design. design included two temperature conditions (37 °C as 42 to simulate heat stress) pharmacological treatments: chloroquine (CQ), an autophagy inhibitor, rapamycin (RAPA), activator. Our results showed initially suppresses activity, which subsequently enhanced with prolonged exposure. The pharmacologic modulation autophagy, through activation or inhibition, resulted corresponding upregulation downregulation epithelial cells' (EECs) receptivity markers. overexpression ATG7 partially reversed HS-induced Additionally, TJP1, tight-junction marker, was degraded under genetic but accumulated more EECs pre-treated CQ. These findings suggest plays protective role maintaining offers valuable insights into proposes potential strategy mitigate adverse goat reproduction.

Language: Английский

A novel nuclear receptor NR1D1 suppresses HSD17B12 transcription to regulate granulosa cell apoptosis and autophagy via the AMPK pathway in sheep DOI
Yu Cai, Hui Xu, Kaiping Deng

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 141271 - 141271

Published: Feb. 1, 2025

Language: Английский

Citations

0
Identifying Shared Molecular Landscape between Uterine Fibroids and Recurrent Implantation Failure: An Integrated Bioinformatics Study DOI Creative Commons
Maryamsadat Hosseini, Basireh Bahrami, Mohammad Reza Zare

et al.

Heliyon, Journal Year: 2025, Volume and Issue: unknown, P. e43006 - e43006

Published: Feb. 1, 2025

Language: Английский

Citations

0
IGF2BP2 regulates the proliferation and migration of endometrial stromal cells through the PI3K/AKT/mTOR signaling pathway in Hu sheep DOI
Kaiping Deng, Xiaodan Li, Zhipeng Liu

et al.

Journal of Animal Science, Journal Year: 2024, Volume and Issue: 102

Published: Jan. 1, 2024

Abstract Insulin-like growth factor 2 mRNA binding protein (IGF2BP2), a significant member of the conserved RNA-binding family, plays various roles in numerous physiological and pathological processes. However, specific function IGF2BP2 regulating endometrial sheep remains largely unknown. In this study, we observed upregulation abundance endometrium during luteal phase compared to follicular Hu sheep. The knockdown resulted accelerated cell proliferation migration stromal cells (ESCs). Moreover, RNA sequencing analysis revealed that genes with significantly altered expression were predominantly enriched receptivity-related signaling pathways, such as cytokine-cytokine receptor interaction, NOD-like receptor, PI3K-AKT, JAK-STAT pathway. Additionally, increased matrix metalloprotein 9 (MMP9), vascular endothelial factor, prolactin (PRL) ESCs. was also stimulate PI3K/AKT/mTOR pathway by upregulating integrin β4 (ITGB4) expression. Notably, downregulation ITGB4 attenuates knockdown-induced facilitation ESCs inhibiting Collectively, these findings highlight important role function, particularly through modulation ESC via

Language: Английский

Citations

1
Heat Stress Impairs Endometrial Function During Implantation by Regulating Autophagy in Hainan Black Goat DOI Creative Commons
Xiaoping Li,

Yanyu Sun,

Min Yi

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(22), P. 3213 - 3213

Published: Nov. 8, 2024

Heat stress (HS) is a significant factor that adversely affects the health, welfare, and productivity of domestic animals, particularly impacting embryo implantation rates. However, effects HS on endometrial function during peri-implantation period in Hainan black goats remain unclear. This study explores influence endometrium these goats. We collected uterine tissue samples from both control heat-stressed performed vitro experiments using 2 × factorial design. design included two temperature conditions (37 °C as 42 to simulate heat stress) pharmacological treatments: chloroquine (CQ), an autophagy inhibitor, rapamycin (RAPA), activator. Our results showed initially suppresses activity, which subsequently enhanced with prolonged exposure. The pharmacologic modulation autophagy, through activation or inhibition, resulted corresponding upregulation downregulation epithelial cells' (EECs) receptivity markers. overexpression ATG7 partially reversed HS-induced Additionally, TJP1, tight-junction marker, was degraded under genetic but accumulated more EECs pre-treated CQ. These findings suggest plays protective role maintaining offers valuable insights into proposes potential strategy mitigate adverse goat reproduction.

Language: Английский

Citations

0