Advancements in 3D Cell Culture Models for Drug Discovery and Disease Modeling DOI
Rea Barlas

Next frontier., Journal Year: 2024, Volume and Issue: 8(1), P. 203 - 203

Published: Nov. 28, 2024

The advent of 3D cell culture models has revolutionized drug discovery and disease modeling, offering more physiologically relevant systems compared to traditional 2D cultures. These advanced mimic the in vivo microenvironment, incorporating critical cellular interactions, extracellular matrix components, gradients oxygen nutrients. This research explores development application technologies, such as organoids, spheroids, bioprinted tissues, studying mechanisms evaluating efficacy. study highlights advantages these recapitulating complex biological processes, including tumor microenvironments, organ development, tissue-specific responses therapeutic agents. By employing cutting-edge techniques microfluidics, imaging, computational aims optimize for high-throughput screening personalized medicine. findings underscore transformative potential bridging gap between preclinical studies clinical outcomes, reducing costs improving patient-specific approaches.

Language: Английский

Advances in induced pluripotent stem cell‐derived cardiac myocytes: technological breakthroughs, key discoveries and new applications DOI Creative Commons
Colleen E. Clancy, Luis F. Santana

The Journal of Physiology, Journal Year: 2024, Volume and Issue: 602(16), P. 3871 - 3892

Published: July 20, 2024

Abstract A transformation is underway in precision and patient‐specific medicine. Rapid progress has been enabled by multiple new technologies including induced pluripotent stem cell‐derived cardiac myocytes (iPSC‐CMs). Here, we delve into these advancements their future promise, focusing on the efficiency of reprogramming techniques, fidelity differentiation lineage, functional characterization resulting myocytes, many applications silico models to understand general mechanisms controlling excitation–contraction coupling health disease. Furthermore, explore current potential iPSC‐CMs both research clinical settings, underscoring far‐reaching implications this rapidly evolving field. image

Language: Английский

Citations

4
Application of CRISPR technology in cardiomyocyte electrophysiology DOI Creative Commons
Yukun Han, Zhe Huang,

Bojun Zhang

et al.

Theoretical and Natural Science, Journal Year: 2025, Volume and Issue: 82(1), P. 12 - 18

Published: Jan. 15, 2025

This paper explores the rising prevalence of cardiovascular disease, highlighting an anticipated increase in patient numbers worldwide. Three major disorders are emphasized: 1) congestive heart failure (CHF), 2) coronary atherosclerosis (CA), and 3) arrhythmia, with arrhythmia identified as especially critical due to its impact on electrophysiology. Advances molecular biology, particularly CRISPR-Cas9 gene-editing technology, have introduced promising therapeutic avenues for these conditions. has demonstrated effectiveness reducing specific cytoplasmic signaling interactions, enhancing division rate human mesenchymal stem cells (hMSCs) within connective tissue. intervention targets partially modifies Toll-like receptor 4 (TLR4) pathway, leading reduced secretion certain factors. As a result, cardiomyocytes (CMs) can undergo improved division, promoting stable electrophysiological functions essential managing arrhythmia. Further, CRISPR-Cas9s capacity precise double screening knockout TLR4-associated sequences holds potential symptom alleviation decreased incidence, representing significant advance cardiac disorder therapeutics.

Language: Английский

Citations

0
From injury to repair: the therapeutic potential of induced pluripotent stem cells in heart failure DOI Creative Commons
Tadahisa Sugiura, Sheeza Nawaz, Dhienda C. Shahannaz

et al.

Regenerative medicine reports ., Journal Year: 2025, Volume and Issue: 2(1), P. 22 - 30

Published: March 1, 2025

Heart failure is a complex clinical syndrome characterized by the heart’s inability to pump sufficient blood meet body’s metabolic demands, resulting in symptoms such as dyspnea, fatigue, and fluid retention. Despite significant advances pharmacological treatments device therapies, prognosis for patients with advanced heart remains poor. This underscores urgent need innovative regenerative therapies aimed at restoring cardiac function improving quality of life suffering from this debilitating condition. article provides comprehensive overview pathophysiologic mechanisms underlying end-stage failure, which include myocardial systolic diastolic dysfunction, neuroendocrine activation, inflammation, oxidative stress. Collectively, these lead progressive deterioration structure function, culminating failure. The also examines differences between ischemic non-ischemic cardiomyopathies their implications treatment strategies prognosis. Furthermore, review explores application medicine focusing on its potential repair functional recovery. In field medicine, induced pluripotent stem cells derived cardiomyocytes are considered cutting-edge technologies repair. Induced can differentiate into cardiomyocytes, thereby offering new hope regeneration. However, current research faces several challenges, including maturity integration immunogenicity concerns, difficulties scaling up production applications. Additionally, ethical issues related cell technology, sourcing tumorigenic risks, must be carefully addressed. highlights technology treating while emphasizing complexities involved transitioning laboratory practice.

Language: Английский

Citations

0
Application of new approach methodologies for nonclinical safety assessment of drug candidates DOI
Mario Beilmann,

Karissa Adkins,

Harrie C. M. Boonen

et al.

Nature Reviews Drug Discovery, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Language: Английский

Citations

0
Differentiation of Pluripotent Stem Cells for Disease Modeling: Learning from Heart Development DOI Creative Commons
Congwu Chi, Truman J. Roland, Kunhua Song

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(3), P. 337 - 337

Published: March 5, 2024

Heart disease is a pressing public health problem and the leading cause of death worldwide. The heart first organ to gain function during embryogenesis in mammals. development involves cell determination, expansion, migration, crosstalk, which are orchestrated by numerous signaling pathways, such as Wnt, TGF-β, IGF, Retinoic acid pathways. Human-induced pluripotent stem cell-based platforms emerging promising approaches for modeling vitro. Understanding pathways that essential cardiac has shed light on molecular mechanisms congenital defects postnatal diseases, significantly advancing model diseases. This review summarizes crucial discusses how these findings improve strategies human

Language: Английский

Citations

2
Mapping the landscape of PSC-CM research through bibliometric analysis DOI Creative Commons
Jun Li,

Shangting Han,

Fengxu Yu

et al.

Frontiers in Cardiovascular Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Oct. 10, 2024

The discovery of pluripotent stem cell-derived cardiomyocytes (PSC-CMs) has not only deepened our understanding the pathogenesis and progression heart disease, but also advanced development engineered cardiac tissues, regenerative therapy, drug cardiotoxicity assessment drugs. This study aims to visualize developmental trajectory PSC-CM research over past 18 years identify emerging frontiers challenges.

Language: Английский

Citations

0
Advancements in 3D Cell Culture Models for Drug Discovery and Disease Modeling DOI
Rea Barlas

Next frontier., Journal Year: 2024, Volume and Issue: 8(1), P. 203 - 203

Published: Nov. 28, 2024

The advent of 3D cell culture models has revolutionized drug discovery and disease modeling, offering more physiologically relevant systems compared to traditional 2D cultures. These advanced mimic the in vivo microenvironment, incorporating critical cellular interactions, extracellular matrix components, gradients oxygen nutrients. This research explores development application technologies, such as organoids, spheroids, bioprinted tissues, studying mechanisms evaluating efficacy. study highlights advantages these recapitulating complex biological processes, including tumor microenvironments, organ development, tissue-specific responses therapeutic agents. By employing cutting-edge techniques microfluidics, imaging, computational aims optimize for high-throughput screening personalized medicine. findings underscore transformative potential bridging gap between preclinical studies clinical outcomes, reducing costs improving patient-specific approaches.

Language: Английский

Citations

0