Multi-Target Protective Effects of Sanghuangporus sanghuang Against 5-Fluorouracil-Induced Intestinal Injury Through Suppression of Inflammation, Oxidative Stress, Epitheli-Al-Mesenchymal Transition, and Tight Junction

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3444 - 3444

Published: April 7, 2025

Sanghuang (Sanghuangporus sanghuang, SS) is a medicinal fungus with multiple pharmacological effects, including antioxidant, anti-inflammatory, immune-boosting, and anti-cancer activities. 5-fluorouracil (5-FU) commonly used chemotherapeutic agent for the treatment of colorectal cancer. It primarily exerts its antitumor effect by inhibiting DNA RNA synthesis, leading to cell apoptosis. However, it frequently induces adverse effects These issues limit clinical application 5-FU. This research aims determine potential SS as therapeutic in reducing 5-FU-induced intestinal mucositis mouse model. The results indicated that 5-FU administration significantly increased diarrhea severity, reduced colon length, caused small villus atrophy, disrupted architecture, led insufficient crypt proliferation, resulted weight loss. also upregulated inflammatory responses, apoptosis, oxidative stress, epithelial-mesenchymal transition (EMT) pathways, integrity mucosal tight junction, while elevating pro-inflammatory cytokines antioxidant capacity. ameliorating alleviating impacts on mucosa. In conclusion, this investigation provides first evidence protective mucositis. findings suggest application, offering promising strategy chemotherapy improving quality life cancer patients.

Language: Английский

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Bibliometric analysis of research trends and prospective directions of Akkermansia muciniphila from 2010 to 2024

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 16, 2025

Akkermansia muciniphila (A. muciniphila) is an emerging probiotic with potential impact on human health, and there a growing interest in this area, but overall analysis of research trends lacking. This study conducted detailed bibliometric visualization A. to examine the current status, hotspots, trends, aiming inform future directions. utilized Web Science database search from 2010 2024. Bibliometric was using CiteSpace VOSviewer software generate yearly publication contributions by countries, institutions, distinguished researchers, as well key themes influential researches. aimed visualize explore literature over past 15 years, guiding researches identifying gaps field intestinal flora muciniphila. We searched total 4,423 related publications. Wei Chen, Willem de Vos Patrice D. Cani are primary contributors 's research. The top contributing countries institutions China, United States, South Korea, Spain, Italy, centers such Chinese Academy Sciences, Zhejiang University, University Copenhagen, Helsinki being main contributors. Current hotspots focus molecular biology muciniphila, its role barrier maintenance, immune response, for regulating treating digestive metabolic diseases, cancer, fatty liver disease, inflammatory bowel etc., through bile acid metabolism, extracellular vesicles, insulin resistance. Our synthesizes various disease areas suggests enhancing collaboration among authors advance muciniphila-related clinical basic research, efficacy variety diseases effects commonly used medications fill field, provide valid evidence development novel supplement.

Language: Английский

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Bacillus Coagulans BC99 Protects Ionizing Radiation‐Induced Intestinal Injury and Modulates Gut Microbiota and Metabolites in Mice

Molecular Nutrition & Food Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

ABSTRACT The gastrointestinal tract is highly sensitive to ionizing radiation (IR), which causes radiation‐induced intestinal injury (RIII). There are no effective drugs available for RIII in routine clinical treatment, a major limiting factor during the process of radiotherapy pelvic abdominal malignancies. In this study, we aimed elucidate potential probiotic Bacillus coagulans BC99 ( B.coagulans BC99) preventing RIII. C57BL/6J mice were gavage‐administered with 30 days and then exposed single dose 12 Gy x‐ray whole irradiation (WAI). treatment could mitigate by weight loss, maintaining integrity structure barrier, improving inflammatory symptoms, modulating oxidative stress, regulating composition gut microbiota, thereby reestablishing homeostasis. addition, radioprotective mechanism was closely related microbiota‐derived metabolites. This study offers novel perspective advancing probiotic‐based treatments enhancing strategies prevention

Language: Английский

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Human gut microbiota-reactive DP8a regulatory T cells prevent acute graft-versus-host disease in a CD73-dependent manner

JCI Insight, Journal Year: 2024, Volume and Issue: 9(18)

Published: Aug. 1, 2024

Graft-versus-host disease (GvHD) is a life-threatening complication frequently occurring following allogeneic hematopoietic stem cell transplantation (allo-HSCT). Since gut microbiota and regulatory T cells (Tregs) are believed to play roles in GvHD prevention, we investigated whether DP8a Tregs, which have previously described harbor TCR-specificity for the commensal Faecalibacterium prausnitzii, could protect against GvHD, thereby linking its effect on GvHD. We observed decrease CD73+ DP8α Treg frequency allo-HSCT patients at 1-month post-transplantation, was associated with aGvHD development as compared aGvHD-free patients, without being correlated hematological disease's relapse. Importantly, CD73 activity shown be critical DP8αTreg suppressive function. Moreover, of host-reactive Tregs also lower embody protective mechanism responsible maintenance these subset GvHD-free patients. showed that human protected mice xeno-GvHD through limiting deleterious inflammation preserving integrity. Altogether, results demonstrated mediate prevention CD73-dependent manner, likely host-reactivity, advocating use innovative therapeutic strategies preclude aGvHD-related inflammation.

Language: Английский

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The Use of Polysaccharide AOP30 from the Rhizome of Alpinia officinarum Hance to Alleviate Lipopolysaccharide-Induced Intestinal Epithelial Barrier Dysfunction and Inflammation via the TLR4/NfκB Signaling Pathway in Caco-2 Cell Monolayers

Nutrients, Journal Year: 2024, Volume and Issue: 16(13), P. 2151 - 2151

Published: July 5, 2024

Alpinia officinarum Hance is rich in carbohydrates and flavored by natives. The polysaccharide fraction 30 purified from the rhizome of A. (AOP30) shows excellent immunoregulatory ability when administered to regulate immunity. However, effect AOP30 on intestinal epithelial barrier not well understood. Therefore, aim this study investigate protective using a lipopolysaccharide (LPS)-induced dysfunction model further explore its underlying mechanisms. Cytotoxicity, transepithelial electrical resistance (TEER) values, Fluorescein isothiocyanate (FITC)–dextran flux are measured. Simultaneously, protein mRNA levels tight junction (TJ) proteins, including zonula occludens-1 (ZO-1), Occludin, Claudin-1, determined Western blotting reverse-transcription quantitative polymerase chain reaction methods, respectively. results indicate that restores LPS-induced decrease TEER value cell viability. Furthermore, it increases expression ZO-1, Claudin-1. Notably, ZO-1 primary altered response dysfunction. Additionally, downregulates production TNFα via Toll-like receptor 4 (TLR4)/NF-κB signaling pathway. Collectively, findings can be developed as functional food ingredient or natural therapeutic agent for addressing It sheds light role improving function.

Language: Английский

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Purinergic Agonists Increase [Ca²⁺]_i in Rat Conjunctival Goblet Cells Through Ryanodine Receptor Type 3 (RyR3)

AJP Cell Physiology, Journal Year: 2024, Volume and Issue: 327(3), P. C830 - C843

Published: Aug. 5, 2024

ATP and benzoylbenzoyl-ATP (BzATP) increase free cytosolic Ca

Language: Английский

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