Inflammopharmacology, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 17, 2024
Language: Английский
Journal of Orthopaedic Translation, Journal Year: 2024, Volume and Issue: 48, P. 123 - 132
Published: Aug. 7, 2024
Pain is the leading symptom for most individuals with osteoarthritis (OA), a complex condition marked by joint discomfort. Recently, dynamic interplay between nervous and immune systems has become focal point understanding pain regulation. Despite this, there still substantial gap in our comprehensive of neuroimmune interactions their effects on OA. This review examines bidirectional influences cells nerves OA progression. It explores current approaches that target pathways, including promoting M2 macrophage polarization specific neuronal receptor targeting, effective reduction.
Language: Английский
Citations
5
Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 179, P. 117364 - 117364
Published: Sept. 2, 2024
Osteoarthritis (OA) is a progressive degenerative disease resulting in joint deterioration. It whole organ characterized by cartilage degeneration and varying degrees of synovitis, involving pathological changes all tissues, such as cartilage, subchondral bone, ligaments, meniscus, synovium, infrapatellar fat pad (IPFP). IPFP the largest adipose tissue structure knee composed cells, immune cells blood vessels. Moreover, located close to bone surface so that it may reduce impact loading absorb forces generated through joint, have protective role health. has been shown release various cytokines adipokines play pro-inflammatory pro-catabolic roles promoting OA progression. Intra-articular injections IPFP-derived mesenchymal stem exosomes pain prevent progression patients with OA. Previous studies biphasic effect on This article reviews latest research progress IPFP, discusses mechanism OA, provide new intervention strategies for treatment will also discuss handling during procedure total arthroplasty.
Language: Английский
Citations
5
Journal of Extracellular Vesicles, Journal Year: 2025, Volume and Issue: 14(3)
Published: March 1, 2025
ABSTRACT Mesenchymal stem/stromal cells (MSCs) are a valuable source of paracrine factors, as they have remarkable secretory capacity, and there is sizeable knowledge base to develop industrial clinical production protocols. Promising cell‐free approaches for tissue regeneration immunomodulation driving research towards secretome applications, among which extracellular vesicles (EVs) steadily gaining attention. However, the manufacturing application EVs limited by insufficient yields, gaps, low standardization. Facing these limitations, hydrogels represent versatile three‐dimensional (3D) culture platform that can incorporate matrix (ECM) components mimic natural stem cell environment in vitro; via niche‐mimicking properties, regulate MSCs’ morphology, adhesion, proliferation, differentiation secretion capacities. impact hydrogel's architectural, biochemical biomechanical properties on remains poorly understood, field still its infancy interdependency parameters compromises comparability studies. Therefore, this review summarizes discusses reported effects hydrogel encapsulation MSC‐EVs. Considering cell‐material interactions overall activity MSCs, we identify persistent challenges from standardization process control, outline future paths research, such synergic use bioreactors enhance MSC‐EV generation.
Language: Английский
Citations
0
Bioengineering, Journal Year: 2025, Volume and Issue: 12(5), P. 525 - 525
Published: May 15, 2025
Osteoarthritis (OA) is a chronic degenerative joint disease characterized by low-grade inflammation, cartilage breakdown, and persistent pain. Despite its prevalence, current therapeutic strategies primarily focus on symptom management rather than modifying progression. Monoclonal antibodies cytokine inhibitors targeting inflammatory pathways, including TNF-α IL-1, have shown promise but remain limited inconsistent efficacy safety concerns. Long-acting biologic therapies—ranging from extended-release formulations, such as monoclonal inhibitors, to gene therapy approaches—have emerged promising enhance treatment durability improve patient outcomes. Extracellular vesicles (EVs) gained particular attention novel delivery platform due their inherent stability, biocompatibility, ability transport cargo, biologics immunomodulatory agents, directly tissues. This review explores the evolving role of EVs in OA treatment, highlighting extend drug half-life, targeting, modulate responses. Additionally, for EV engineering, endogenous exogenous cargo loading, genetic modifications, biomaterial-based systems, are discussed.
Language: Английский
Citations
0
Bioengineering, Journal Year: 2024, Volume and Issue: 11(10), P. 961 - 961
Published: Sept. 26, 2024
Osteoarthritis (OA) is a prominent cause of disability, and has severe social economic ramifications across the globe. The main driver OA’s pervasiveness fact that no current medical interventions exist to reverse or even attenuate degeneration cartilage within articular joint. Crucial for cell-to-cell communication, extracellular vesicles (EVs) contribute OA progression through delivery bioactive molecules in inflammatory microenvironment. By repurposing this acellular means signal transmission, therapeutic drugs may be administered degenerated tissue hopes encouraging regeneration. Positive outcomes are apparent vivo studies on subject; however, therapy prove itself clinical world, efforts towards standardizing characterization, application, biological contents, dosage essential.
Language: Английский
Citations
3
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Nov. 27, 2024
Osteoarthritis (OA) represents a significant global health burden without known disease modifying agent thereby necessitating pursuit of innovative therapeutic approaches. The infrapatellar fat pad (IFP) serves as reservoir mesenchymal stem/stromal cells (MSC), and with adjacent synovium plays key roles in joint affecting local inflammatory responses. Therapeutically, IFP-MSC have garnered attention for their potential OA treatment due to immunomodulatory regenerative properties. However, optimizing efficacy necessitates comprehensive understanding how growth medium inflammatory/hormonal priming influence behavior. In this study, we isolated expanded three different media: DMEM + 10% fetal bovine serum (FBS), human platelet lysate (HPL), xeno-/serum-free synthetic (XFSF) medium. Subsequently, were induced an inflammatory/fibrotic cocktail (TIC) or oxytocin (OXT). We evaluated various parameters including kinetics, phenotype, capacity, gene expression, macrophage polarization capacity. Our results revealed differences the behavior MSC cultured media. HPL XFSF exhibited superior kinetics colony-forming abilities compared those FBS. Furthermore, both media enhanced expression markers (> 90%) potentiated Notably, XFSF-conditioned demonstrated highest attenuation peripheral blood mononuclear cell (PBMC) proliferation, indicating robust immunosuppressive Additionally, TIC further augmented functionality MSC, exhibiting suppression PBMC proliferation upon priming. Of particular interest, analysis distinct patterns OXT only induced, upregulation genes associated functions. promoted M2 macrophages, suggesting strategy immune-mediated conditions OA. findings highlight critical on potential. offer promising alternatives FBS, enhancing Moreover, novel approach augment immunomodulation promote polarization, providing insights into strategies other conditions.
Language: Английский
Citations
3
Journal of Orthopaedic Research®, Journal Year: 2024, Volume and Issue: 43(2), P. 457 - 465
Published: Oct. 13, 2024
Extracellular vesicles (EVs) derived from endometrial-derived mesenchymal stem/stromal cells (eMSC) play a crucial role in tissue repair due to their immunomodulatory and reparative properties. Given these properties, eMSC EVs may offer potential benefits for meniscal repair. The meniscus, being partly vascularized, relies on diffusivity solute trafficking. This study focuses transport properties characterization within fibrocartilage that remains unknown. Specifically, were isolated Crude CD146
Language: Английский
Citations
2
Inflammopharmacology, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 17, 2024
Language: Английский
Citations
0