Extracellular Vesicles from Different Mesenchymal Stem Cell Types Exhibit Distinctive Surface Protein Profiling and Molecular Characteristics: A Comparative Analysis DOI Open Access
Antonio Fernández-Pérez, Azucena Herrera-González, Edgar J. López‐Naranjo

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3393 - 3393

Published: April 4, 2025

The current medical need to respond different diseases has sparked great interest in extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) due their regenerative potential and as drug carriers by playing a critical role cell-cell communication. However, heterogeneity, there is no standardized universal method for identification characterization, which limits clinical application. This study, following the recommendations methodologies proposed MISEV2023 characterization of EVs, shows first time detailed morphological, protein, biochemical comparison between EVs three MSCs sources (placenta, endometrium, dental pulp). information obtained applied assays suggests that are substantial differences one source another. It also offers valuable insights provide guidelines ease profiling therefore improve selection, order speed up use application; additionally, knowledge each test could facilitate new researchers field choose specific cell obtain select appropriate methods necessary according requirements.

Language: Английский

Exosomes in Ocular Health: Recent Insights into Pathology, Diagnostic Applications and Therapeutic Functions DOI Creative Commons
Noelia Blanco-Agudín, Suhui Ye, Sara González-Fernández

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(1), P. 233 - 233

Published: Jan. 19, 2025

Exosomes are extracellular vesicles ranging from 30 to 150 nm in diameter that contain proteins, nucleic acids and other molecules. Produced by virtually all cell types, they travel throughout the body until reach their target, where can trigger a wide variety of effects transferring molecular cargo recipient cells. In context ocular physiology, exosomes play very important role embryological development, regulation homeostasis immune system, which is crucial for normal vision. Consequently, pathological situations, also undergo modifications terms quantity, composition content, depending on etiology disease. However, mechanisms contribute pathology has not yet been studied depth, many questions remain unanswered. This review aims summarize most recent knowledge function system healthy individuals during processes degenerative, infectious, neurodegenerative, vascular inflammatory nature, such as keratoconus, keratitis, glaucoma, diabetic retinopathy uveitis. Furthermore, given unique characteristics, potential diagnostic biomarkers or therapeutic agents application clinical ophthalmology explored, along with main limitations researchers face today field.

Language: Английский

Citations

0
PAX3 expression patterns in ocular surface melanocytes DOI Creative Commons

Eva Ulrich,

Sebastian Kistenmacher,

Gottfried Martin

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 11, 2025

Abstract PAX3, a transcription factor essential for neural crest development and melanocyte progenitors, is expressed in various melanocytic tissues. However, its role ocular surface tissues remains poorly understood. This study investigated the expression patterns of PAX3 limbal stem cell niche, specifically epithelial progenitor cells (LEPC), melanocytes (LM), mesenchymal (LMSC). Additionally, was studied conjunctival/limbal melanoma specimens. Immunohistochemical analysis revealed predominant LM as well conjunctival melanocytes, suggesting distinct roles regulation maintenance. Notably, significantly upregulated compared to healthy counterparts, with co-localizing markers (Melan-A, HMB45, SOX10) proliferation marker Ki-67 cells. These findings suggests that while restricted limbal/conjunctival dysregulation may play crucial development. Further investigation into mechanisms by which influences corneal pathophysiology contributes pathogenesis could identify potential therapeutic targets this aggressive malignancy.

Language: Английский

Citations

0
Proteomic Insights into Human Limbal Epithelial Progenitor-Derived Small Extracellular Vesicles DOI Creative Commons

Moritz Vincent Braunsperger,

Gottfried Martin,

Tabea Herzig

et al.

Stem Cell Reviews and Reports, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Language: Английский

Citations

0
Extracellular Vesicles from Different Mesenchymal Stem Cell Types Exhibit Distinctive Surface Protein Profiling and Molecular Characteristics: A Comparative Analysis DOI Open Access
Antonio Fernández-Pérez, Azucena Herrera-González, Edgar J. López‐Naranjo

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3393 - 3393

Published: April 4, 2025

The current medical need to respond different diseases has sparked great interest in extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) due their regenerative potential and as drug carriers by playing a critical role cell-cell communication. However, heterogeneity, there is no standardized universal method for identification characterization, which limits clinical application. This study, following the recommendations methodologies proposed MISEV2023 characterization of EVs, shows first time detailed morphological, protein, biochemical comparison between EVs three MSCs sources (placenta, endometrium, dental pulp). information obtained applied assays suggests that are substantial differences one source another. It also offers valuable insights provide guidelines ease profiling therefore improve selection, order speed up use application; additionally, knowledge each test could facilitate new researchers field choose specific cell obtain select appropriate methods necessary according requirements.

Language: Английский

Citations

0