Single-Cell Sequencing Combined with Transcriptome Sequencing to Explore the Molecular Mechanisms Related to Skin Photoaging DOI Creative Commons
Xinru Hu, Shuang Du, Meng Chen

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 11137 - 11160

Published: Dec. 1, 2024

Background:The aging of skin is a diversified biological phenomenon, influenced by combination genetic and environmental factors.However, the specific mechanism photoaging not yet completely elucidated.Methods: Gene expression profiles for patients were obtained from Expression Omnibus (GEO) collection.We conducted single-cell intercellular communication investigations to identify potential gene sets.Predictive models created using LASSO regression.The relationships between genes immune cells investigated single sample set enrichment analysis (ssGSEA) variance (GSVA).The molecular processes important studied analysis.A miRNA network was look target miRNAs connected with genes, transcriptional regulation used related transcription factors.Finally, merging co-expression networks drug prediction shows pathways treatment targets.Furthermore, we validated role key cell infiltration, Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) pathway in photoaging, which identified through bioinformatics analysis, vivo reverse quantitative PCR (RT-qPCR), immunofluorescence labeling, Western blotting.Results: This study discovered three including Atp2b1, Plekho2, Tspan13, perform crucial functions process.Immune infiltration showed increased M1 macrophages CD4 memory T group.Further signaling indicated that these are enriched multiple metabolic pathways.The significant roles AMPK vivo. Conclusion:This research revealed underlying mechanisms indicating such as Atp2b1 Tspan13 play pathways.These findings provide new theory prospective targets advancement relevant drugs.

Language: Английский

Single-Cell Sequencing Combined with Transcriptome Sequencing to Explore the Molecular Mechanisms Related to Skin Photoaging DOI Creative Commons
Xinru Hu, Shuang Du, Meng Chen

et al.

Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 11137 - 11160

Published: Dec. 1, 2024

Background:The aging of skin is a diversified biological phenomenon, influenced by combination genetic and environmental factors.However, the specific mechanism photoaging not yet completely elucidated.Methods: Gene expression profiles for patients were obtained from Expression Omnibus (GEO) collection.We conducted single-cell intercellular communication investigations to identify potential gene sets.Predictive models created using LASSO regression.The relationships between genes immune cells investigated single sample set enrichment analysis (ssGSEA) variance (GSVA).The molecular processes important studied analysis.A miRNA network was look target miRNAs connected with genes, transcriptional regulation used related transcription factors.Finally, merging co-expression networks drug prediction shows pathways treatment targets.Furthermore, we validated role key cell infiltration, Adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK) pathway in photoaging, which identified through bioinformatics analysis, vivo reverse quantitative PCR (RT-qPCR), immunofluorescence labeling, Western blotting.Results: This study discovered three including Atp2b1, Plekho2, Tspan13, perform crucial functions process.Immune infiltration showed increased M1 macrophages CD4 memory T group.Further signaling indicated that these are enriched multiple metabolic pathways.The significant roles AMPK vivo. Conclusion:This research revealed underlying mechanisms indicating such as Atp2b1 Tspan13 play pathways.These findings provide new theory prospective targets advancement relevant drugs.

Language: Английский

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