Quercetin in Shengxian Decoction exhibits anti-ferroptosis protective roles in a myocardial infarction model via targeting DPP4/ HMOX1, based on network pharmacology and molecular docking DOI Creative Commons
Yu Zhai, J. F. Fu,

Jianfei Yang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 29, 2025

Background Myocardial infarction (MI) is characterized by high morbidity. In this study, we aimed to elucidate potential targets of Shengxian Decoction (SXD) against MI. Methods Pairing SXD active ingredients and MI was conducted using the Chinese Medicine System Pharmacological Database, Gene Expression Omnibus (GEO), STRING databases. The effects on were validated in vitro . Molecular docking verified cellular thermal shift assay (CETSA). Results A total 40 28 MI-related obtained. Cross-analysis cell death-related genes identified two crucial ferroptosis-related targets, namely, dipeptidyl peptidase 4 (DPP4) heme oxygenase 1 (HMOX1). cobalt chloride (CoCl 2 )-induced hypoxic H9c2 cells, could remarkably improve viability inhibit death. Meanwhile, treatment significantly affected markers cells. CETSA results showed that quercetin had good binding activity with DPP4 HMOX1. Conclusion Important ingredient exert anti-ferroptosis protective roles through targeting (DPP4/HMOX1), thereby contributing role

Language: Английский

TF and TFRC regulate ferroptosis in swine testicular cells through the JNK signaling pathway DOI

Yuanjie Zhao,

Ge Qin,

Weimin Fan

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142369 - 142369

Published: March 1, 2025

Language: Английский

Citations

0
Quercetin in Shengxian Decoction exhibits anti-ferroptosis protective roles in a myocardial infarction model via targeting DPP4/ HMOX1, based on network pharmacology and molecular docking DOI Creative Commons
Yu Zhai, J. F. Fu,

Jianfei Yang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 29, 2025

Background Myocardial infarction (MI) is characterized by high morbidity. In this study, we aimed to elucidate potential targets of Shengxian Decoction (SXD) against MI. Methods Pairing SXD active ingredients and MI was conducted using the Chinese Medicine System Pharmacological Database, Gene Expression Omnibus (GEO), STRING databases. The effects on were validated in vitro . Molecular docking verified cellular thermal shift assay (CETSA). Results A total 40 28 MI-related obtained. Cross-analysis cell death-related genes identified two crucial ferroptosis-related targets, namely, dipeptidyl peptidase 4 (DPP4) heme oxygenase 1 (HMOX1). cobalt chloride (CoCl 2 )-induced hypoxic H9c2 cells, could remarkably improve viability inhibit death. Meanwhile, treatment significantly affected markers cells. CETSA results showed that quercetin had good binding activity with DPP4 HMOX1. Conclusion Important ingredient exert anti-ferroptosis protective roles through targeting (DPP4/HMOX1), thereby contributing role

Language: Английский

Citations

0