Advancements in single-cell RNA sequencing and spatial transcriptomics: transforming biomedical research

Acta Biochimica Polonica, Journal Year: 2025, Volume and Issue: 72

Published: Feb. 5, 2025

In recent years, significant advancements in biochemistry, materials science, engineering, and computer-aided testing have driven the development of high-throughput tools for profiling genetic information. Single-cell RNA sequencing (scRNA-seq) technologies established themselves as key dissecting sequences at level single cells. These reveal cellular diversity allow exploration cell states transformations with exceptional resolution. Unlike bulk sequencing, which provides population-averaged data, scRNA-seq can detect subtypes or gene expression variations that would otherwise be overlooked. However, a limitation is its inability to preserve spatial information about transcriptome, process requires tissue dissociation isolation. Spatial transcriptomics pivotal advancement medical biotechnology, facilitating identification molecules such their original context within sections single-cell level. This capability offers substantial advantage over traditional techniques. valuable insights into wide range biomedical fields, including neurology, embryology, cancer research, immunology, histology. review highlights approaches, technological developments, associated challenges, various techniques data analysis, applications disciplines microbiology, neuroscience, reproductive biology, immunology. It critical role characterizing dynamic nature individual

Language: Английский

Volatile Organic Compound–Drug Receptor Interactions: A Potential Tool for Drug Design in the Search for Remedies for Increasing Toxic Occupational Exposure

Processes, Journal Year: 2025, Volume and Issue: 13(1), P. 154 - 154

Published: Jan. 8, 2025

Volatile organic compounds (VOCs) can impact the actions of drugs due to their effects on drug receptors and activities enzymes involved in various metabolic processes, especially those relating gene regulation. They disrupt cellular functions potentially affect human metabolism utilization receptors. mimic or inhibit endogenous ligands, leading carcinogenesis, neurotoxicity, endocrine disruption, respiratory disorders. Chronic exposure VOCs occupation lead an increased generation reactive oxygen species (ROS), which could oxidative stress damage lipids, affecting formation proper functioning regulation, enzyme activity, cell membranes. The presence interfere with activity body’s ability process utilize effectively. This is because such as antioxidant play essential role protection against damage. Therefore, disruptions distort overall health condition through breakdown defense mechanisms. In this study, aim assess effect VOC way forward designing maintaining optimal for workers’ well-being.

Language: Английский

Recent progress in single-cell transcriptomic studies in plants

Plant Biotechnology Reports, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Language: Английский