Purinergic System Transcript Changes in the Dorsolateral Prefrontal Cortex in Suicide and Major Depressive Disorder DOI Open Access
Smita Sahay, Anna Lundh,

Roshan P. Sirole

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1826 - 1826

Published: Feb. 20, 2025

Suicide is a major public health priority, and its molecular mechanisms appear to be related imbalanced purine metabolism in the brain. This exploratory study investigates purinergic gene expression postmortem dorsolateral prefrontal cortex (DLPFC) tissue isolated from subjects with depressive disorder (MDD) who died by suicide (MDD-S, n = 10), MDD did not die (MDD-NS, 6) non-psychiatrically ill controls (CTL, 9–10). Purinergic system transcripts were assayed quantitative polymerase chain reactions (qPCR) superficial deep gray matter as well white DLPFC cortical layers using laser microdissection (LMD). Across all subjects, regardless of sex, P2RY12 (F(2,23) 5.40, p 0.004) P2RY13 (KW statistic 11.82, 0.001) transcript levels significantly greater MDD-S compared MDD-NS subjects. Several other perturbations observed females: NT5E (F(2,10) 13.37, (F(2,9) 3.99, 0.011, controlled for age) was vs. female groups. ENTPD2 5.20, 0.03), ENTPD3 28.99, < 0.0001), among whose elevated CTL Transcripts that exhibited altered included ENTPD2, NT5E, PANX1, (p ≤ 0.05). Our medication analysis revealed these antidepressants. first holistically quantify metabolic pathway utilizing human brain tissue. preliminary findings support evidence implicating changes P2 receptors provide broader dysregulation mood disorders.

Language: Английский

Purinergic System Transcript Changes in the Dorsolateral Prefrontal Cortex in Suicide and Major Depressive Disorder DOI Open Access
Smita Sahay, Anna Lundh,

Roshan P. Sirole

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 1826 - 1826

Published: Feb. 20, 2025

Suicide is a major public health priority, and its molecular mechanisms appear to be related imbalanced purine metabolism in the brain. This exploratory study investigates purinergic gene expression postmortem dorsolateral prefrontal cortex (DLPFC) tissue isolated from subjects with depressive disorder (MDD) who died by suicide (MDD-S, n = 10), MDD did not die (MDD-NS, 6) non-psychiatrically ill controls (CTL, 9–10). Purinergic system transcripts were assayed quantitative polymerase chain reactions (qPCR) superficial deep gray matter as well white DLPFC cortical layers using laser microdissection (LMD). Across all subjects, regardless of sex, P2RY12 (F(2,23) 5.40, p 0.004) P2RY13 (KW statistic 11.82, 0.001) transcript levels significantly greater MDD-S compared MDD-NS subjects. Several other perturbations observed females: NT5E (F(2,10) 13.37, (F(2,9) 3.99, 0.011, controlled for age) was vs. female groups. ENTPD2 5.20, 0.03), ENTPD3 28.99, < 0.0001), among whose elevated CTL Transcripts that exhibited altered included ENTPD2, NT5E, PANX1, (p ≤ 0.05). Our medication analysis revealed these antidepressants. first holistically quantify metabolic pathway utilizing human brain tissue. preliminary findings support evidence implicating changes P2 receptors provide broader dysregulation mood disorders.

Language: Английский

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