Astrocyte-derived exosomes regulate sperm miR-34c levels to mediate the transgenerational effects of paternal chronic social instability stress

Epigenetics, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 27, 2025

The effects of chronically stressing male mice can be transmitted across generations by stress-specific changes in their sperm miRNA content, which induce phenotypes offspring. However, how each stress paradigm alters the levels distinct sets miRNAs is not known. We showed previously that exposure to chronic social instability (CSI) results elevated anxiety and reduced sociability specifically female offspring multiple because it reduces miR-34c stressed males unstressed Here, we describe evidence astrocyte-derived exosomes (A-Exos) carrying mediate CSI has this transgenerational effect on sperm. found decreases carried A-Exos prefrontal cortex amygdala, as well blood males. Importantly, are also these tissues F1 offspring, who despite being exposed stress, exhibit transmit same stress-associated traits Furthermore, restoring content CSI-stressed intravenous injection miR-34c-containing restores These findings reveal an unexpected role for maintaining a process when suppressed mediates example epigenetic inheritance.

Language: Английский

Establishing sex- and age-related reference intervals of serum glial fibrillary acid protein measured by the fully automated lumipulse system

Clinical Chemistry and Laboratory Medicine (CCLM), Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Abstract Objectives To establish the reference intervals (RIs) of serum glial fibrillary acid protein (GFAP) measured by fully automated Lumipulse system. Methods The study population consisted 340 healthy individuals, including 251 blood donors and 89 outpatients, with a median age 56 years. Serum GFAP levels were G assay on platform G1200 (FUJIREBIO Inc., Tokyo, Japan). RIs (2.5th 97.5th percentiles) calculated for overall stratified sex groups. For population, males, females partitions, we employed nonparametric methods, while age-and-sex groups “robust” method, as recommended CLSI. Results RI in whole was 10.4–92.0 pg/mL. When considering differences, showed higher than males across all A positive correlation observed between (Spearman’s rho=0.55, p<0.001). Specifically, biomarker stable until 60 years, individuals aged>60 years demonstrated significantly considerably younger Additionally, 50–60 group, gender-related having increased males. Conclusions are influenced both sex. Accordingly, established GFAP, taking into consideration sex-related differences.

Language: Английский

Systematic review and meta-analysis of microbiota-gut-astrocyte axis perturbation in neurodegeneration, brain injury, and mood disorders

Brain Behavior & Immunity - Health, Journal Year: 2025, Volume and Issue: unknown, P. 101013 - 101013

Published: May 1, 2025

Language: Английский