A novel role of miR-223-3p in reducing NLRP3-mediated inflammation and deep vein thrombosis in a mouse model DOI Creative Commons
Ji Luo, Lei Zheng, Hongyu Zheng

et al.

Science Progress, Journal Year: 2025, Volume and Issue: 108(2)

Published: April 1, 2025

Objective Deep vein thrombosis (DVT) is a global health issue caused by abnormal clotting in deep veins, which can lead to serious complications such as pulmonary embolism. This study the first validate regulatory effect of miR-223-3p on NLRP3 inflammasome mouse model DVT, expanding its potential therapeutic value venous thrombosis-associated inflammation. Methods MicroRNA sequencing and quantitative real-time polymerase chain reaction (qRT–PCR) were conducted assess miRNA expression DVT model. The downstream target miR-223-3p, NLRP3, was identified using prediction databases validated qRT–PCR. Human umbilical endothelial cells (HUVECs) used explore functional relationship between Nlrp3. Results Nlrp3 significantly increased walls mice with DVT. tail injection agomiR-223-3p reduced thrombus formation downregulated Nlrp3, interleukin 6 (Il-6), 1 beta (IL-1beta) Icam-1. In vitro, overexpression Il-6, IL-1beta ICAM-1, whereas antagonized these effects. Additionally, enhanced viability migration LPS-stimulated HUVECs reducing expression. Conclusions Our findings suggest that may play role alleviating inflammation burden downregulating expression, supporting for

Language: Английский

A novel role of miR-223-3p in reducing NLRP3-mediated inflammation and deep vein thrombosis in a mouse model DOI Creative Commons
Ji Luo, Lei Zheng, Hongyu Zheng

et al.

Science Progress, Journal Year: 2025, Volume and Issue: 108(2)

Published: April 1, 2025

Objective Deep vein thrombosis (DVT) is a global health issue caused by abnormal clotting in deep veins, which can lead to serious complications such as pulmonary embolism. This study the first validate regulatory effect of miR-223-3p on NLRP3 inflammasome mouse model DVT, expanding its potential therapeutic value venous thrombosis-associated inflammation. Methods MicroRNA sequencing and quantitative real-time polymerase chain reaction (qRT–PCR) were conducted assess miRNA expression DVT model. The downstream target miR-223-3p, NLRP3, was identified using prediction databases validated qRT–PCR. Human umbilical endothelial cells (HUVECs) used explore functional relationship between Nlrp3. Results Nlrp3 significantly increased walls mice with DVT. tail injection agomiR-223-3p reduced thrombus formation downregulated Nlrp3, interleukin 6 (Il-6), 1 beta (IL-1beta) Icam-1. In vitro, overexpression Il-6, IL-1beta ICAM-1, whereas antagonized these effects. Additionally, enhanced viability migration LPS-stimulated HUVECs reducing expression. Conclusions Our findings suggest that may play role alleviating inflammation burden downregulating expression, supporting for

Language: Английский

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