Deciphering the regulatory programs of RNA binding proteins in rheumatoid arthritis through single-cell transcriptome analysis DOI Creative Commons
Hongbin Luo,

Qunya Zheng,

Yi Zhou

et al.

Acta Materia Medica, Journal Year: 2025, Volume and Issue: 4(1)

Published: Jan. 1, 2025

Single-cell RNA sequencing (scRNA-seq) data from published datasets were obtained to investigate the expression and dysregulation of RNA-binding proteins (RBPs), which are critical for alternative mRNA splicing translational control in rheumatoid arthritis (RA). How RBP regulation differs between RA osteoarthritis (OA) was examined using single-cell sub-clustering. Quantitative polymerase chain reactions (PCRs) performed confirm differentially expressed RBPs fibroblast-like synoviocytes (FLSs) OA-FLSs, as well mice with collagen-induced (CIA) mice. Additionally, bulk RNA-seq collected RBP-alternative event (ASE) co-expression analyses reveal potential regulatory role RA-related on ASEs. Significant variations relative proportions cell subtypes demonstrations OA downregulated outnumbering upregulated each type showing high specificity particular subsets. One hundred five 133 identified fibroblasts. Y-Box binding protein 3 (YBX3) factor 3b subunit 6 (SF3B6) confirmed be RA-FLS CIA mice, while eukaryotic translation initiation 4A1 (EIF4A1) U2 small nuclear auxiliary 1 (U2AF1) RA-FLS. The group displayed stronger interactions compared enhanced signaling pathways, such fibronectin 1-cluster differentiation 44 (FN1-CD44) C-X-C motif chemokine ligand 12-C-X-C receptor 4 (CXCL12-CXCR4). Furthermore, three genes (spectrin repeat containing envelope 2 [SYNE2], S100 calcium A9 [S100A9], interferon induced tetratricopeptide repeats [IFIT3]) four (ribonuclease [RNASE1], granulin [GRN], FN1, sorbin SH3 domain [SORBS2]) co-expressed RA-associated These findings suggest that may contribute development provide targets therapeutic interventions.

Language: Английский

Deciphering the regulatory programs of RNA binding proteins in rheumatoid arthritis through single-cell transcriptome analysis DOI Creative Commons
Hongbin Luo,

Qunya Zheng,

Yi Zhou

et al.

Acta Materia Medica, Journal Year: 2025, Volume and Issue: 4(1)

Published: Jan. 1, 2025

Single-cell RNA sequencing (scRNA-seq) data from published datasets were obtained to investigate the expression and dysregulation of RNA-binding proteins (RBPs), which are critical for alternative mRNA splicing translational control in rheumatoid arthritis (RA). How RBP regulation differs between RA osteoarthritis (OA) was examined using single-cell sub-clustering. Quantitative polymerase chain reactions (PCRs) performed confirm differentially expressed RBPs fibroblast-like synoviocytes (FLSs) OA-FLSs, as well mice with collagen-induced (CIA) mice. Additionally, bulk RNA-seq collected RBP-alternative event (ASE) co-expression analyses reveal potential regulatory role RA-related on ASEs. Significant variations relative proportions cell subtypes demonstrations OA downregulated outnumbering upregulated each type showing high specificity particular subsets. One hundred five 133 identified fibroblasts. Y-Box binding protein 3 (YBX3) factor 3b subunit 6 (SF3B6) confirmed be RA-FLS CIA mice, while eukaryotic translation initiation 4A1 (EIF4A1) U2 small nuclear auxiliary 1 (U2AF1) RA-FLS. The group displayed stronger interactions compared enhanced signaling pathways, such fibronectin 1-cluster differentiation 44 (FN1-CD44) C-X-C motif chemokine ligand 12-C-X-C receptor 4 (CXCL12-CXCR4). Furthermore, three genes (spectrin repeat containing envelope 2 [SYNE2], S100 calcium A9 [S100A9], interferon induced tetratricopeptide repeats [IFIT3]) four (ribonuclease [RNASE1], granulin [GRN], FN1, sorbin SH3 domain [SORBS2]) co-expressed RA-associated These findings suggest that may contribute development provide targets therapeutic interventions.

Language: Английский

Citations

0