The lncRNA Malat1 regulates microvascular function after myocardial infarction in mice via miR-26b-5p/Mfn1 axis-mediated mitochondrial dynamics

Redox Biology, Journal Year: 2021, Volume and Issue: 41, P. 101910 - 101910

Published: Feb. 23, 2021

Myocardial infarction (MI) is a leading cause of cardiovascular mortality globally. The improvement microvascular function critical for cardiac repair after MI. Evidence now points to long non-coding RNAs (lncRNAs) as key regulators remodelling processes. lncRNA Malat1 involved in the development and progression multiple diseases. Studies have shown that closely related regulation endothelial cell regeneration. However, potential molecular mechanisms repairing dysfunction MI remain unreported.The present study found upregulated border zone mouse hearts, well isolated cells (CMECs). Targeted knockdown exacerbated oxidative stress, attenuated angiogenesis perfusion, result decreased mice. Further studies showed silencing obviously inhibited CMEC proliferation, migration tube formation, which was at least part attributed disturbed mitochondrial dynamics activation apoptosis pathway. Moreover, bioinformatic analyses, luciferase assays pull-down indicated acted competing endogenous RNA (ceRNA) miR-26b-5p formed signalling axis with Mfn1 regulate functions. Overexpression markedly reversed injuries were aggravated by via inhibition excessive fragments mitochondria-dependent apoptosis.The elucidated functions microcirculation underlying effects could be its blocking on miR-26b-5p/Mfn1 pathway-mediated apoptosis.

Language: Английский

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Involvement of lncRNAs in cancer cells migration, invasion and metastasis: cytoskeleton and ECM crosstalk

Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)

Published: July 18, 2023

Cancer is the main cause of death worldwide and metastasis a major poor prognosis cancer-associated mortality. Metastatic conversion cancer cells multiplex process, including EMT through cytoskeleton remodeling interaction with TME. Tens thousands putative lncRNAs have been identified, but biological functions most are still to be identified. However, already emerged as key regulators gene expression at transcriptional post-transcriptional level control in spatio-temporal fashion. LncRNA-dependent mechanisms can cell fates during development their perturbed associated onset progression many diseases cancer. LncRNAs involved each step different modes action. The investigation roles could possibly lead identification new biomarkers innovative therapeutic options.

Language: Английский

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Towards Personalized Medicine: Non-Coding RNAs and Endometrial Cancer

Healthcare, Journal Year: 2021, Volume and Issue: 9(8), P. 965 - 965

Published: July 30, 2021

Endometrial cancer (EC) is the most frequent female associated with excellent prognosis if diagnosed at an early stage. The risk factors on which clinical staging based are constantly updated and genetic epigenetic characteristics have recently been emerging as prognostic markers. evidence shows that non-coding RNAs (ncRNAs) play a fundamental role in various biological processes pathogenesis of EC many them also prediction function, remarkable importance defining therapeutic surveillance path patients. Personalized medicine focuses continuous updating identifiable during to tailor treatments This review aims show summary current classification systems encourage integration factors, introducing RNAs, avoid aggressive therapies where not necessary treat strictly monitor subjects greater relapse.

Language: Английский

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Using ncRNAs as Tools in Cancer Diagnosis and Treatment—The Way towards Personalized Medicine to Improve Patients’ Health

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(16), P. 9353 - 9353

Published: Aug. 19, 2022

Although the first discovery of a non-coding RNA (ncRNA) dates back to 1958, only in recent years has complexity transcriptome started be elucidated. However, its components are still under investigation and their identification is one challenges that scientists presently facing. In addition, function far from being fully understood. The portion genome indeed largest, both quantitatively qualitatively. A large fraction these ncRNAs have regulatory role either coding mRNAs or other ncRNAs, creating an intracellular network crossed interactions (competing endogenous networks, ceRNET) fine-tune gene expression health disease. alteration equilibrium among such can enough cause transition disease, but opposite equally true, leading possibility intervening based on mechanisms cure human conditions. this review, we summarize present knowledge mechanisms, illustrating how they used for disease treatment, current pitfalls, roles environmental lifestyle-related contributing factors, addition ethical, legal, social issues arising (improper) use.

Language: Английский

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Exploring the Key Signaling Pathways and ncRNAs in Colorectal Cancer

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(8), P. 4548 - 4548

Published: April 21, 2024

Colorectal cancer (CRC) is the third most prevalent to be diagnosed, and it has a substantial mortality rate. Despite numerous studies being conducted on CRC, remains significant health concern. The disease-free survival rates notably decrease as CRC progresses, emphasizing urgency for effective diagnostic therapeutic approaches. development caused by environmental factors, which mostly lead disruption of signaling pathways. Among these pathways, Wingless/Integrated (Wnt) pathway, Phosphatidylinositol 3-kinase/protein kinase B/mammalian target rapamycin (PI3K/AKT/mTOR) Mitogen-Activated Protein Kinase (MAPK) Transforming Growth Factor-β (TGF-β) p53 pathway are considered important. These pathways also regulated non-coding RNAs (ncRNAs), including microRNAs (miRNAs), long (lncRNAs), circular (circRNAs). They have emerged crucial regulators gene expression in changing their levels. altered patterns ncRNAs been implicated progression development, suggesting potential targets. This review provides an overview five key regulation involved pathogenesis that studied identify promising avenues diagnosis treatment strategies.

Language: Английский

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WTAP-mediated m6A modification of lncRNA Snhg1 improves myocardial ischemia-reperfusion injury via miR-361-5p/OPA1-dependent mitochondrial fusion

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 25, 2024

Abstract Background Myocardial ischemia-reperfusion injury (MIRI) is caused by reperfusion after ischemic heart disease. LncRNA Snhg1 regulates the progression of various diseases. N6-methyladenosine (m 6 A) frequent RNA modification and plays a critical role in MIRI. However, it unclear whether lncRNA MIRI was modified m A methylation. Methods Mouse cardiomyocytes HL-1 cells were utilized to construct hypoxia/reoxygenation (H/R) model. cell viability evaluated utilizing CCK-8 method. Cell apoptosis, mitochondrial reactive oxygen species (ROS), membrane potential (MMP) quantitated flow cytometry. immunoprecipitation dual-luciferase reporter assays applied measure methylation interactions between targeted miRNA or target miRNAs its gene. The I/R mouse model constructed with adenovirus expressing Snhg1. HE TUNEL staining used evaluate myocardial tissue damage apoptosis. Results down-regulated H/R injury, overexpressed suppressed H/R-stimulated ROS level polarization. Besides, could miR-361-5p, miR-361-5p OPA1. Overexpressed polarization though miR-361-5p/OPA1 axis. Furthermore, WTAP induced cells. Moreover, enforced repressed I/R-stimulated apoptosis regulated OPA1 levels. Conclusion WTAP-mediated through modulating production, via axis, providing evidence for as prospective alleviating progression.

Language: Английский

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In silico Analysis and Comprehensive Review of Circular-RNA Regulatory Roles in Breast Diseases; a Step-Toward non-coding RNA Precision

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 263, P. 155651 - 155651

Published: Oct. 19, 2024

Language: Английский

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Non-Coding RNAs in Cancer: Structure, Function, and Clinical Application

Cancers, Journal Year: 2025, Volume and Issue: 17(4), P. 579 - 579

Published: Feb. 8, 2025

We are on the brink of a paradigm shift in both theoretical and clinical oncology. Genomic transcriptomic profiling, alongside personalized approaches that account for individual patient variability, increasingly shaping discourse. Discussions future cancer medicine mainly dominated by potential non-coding RNAs (ncRNAs), which play prominent role progression metastasis formation regulating expression oncogenic or tumor suppressor proteins at transcriptional post-transcriptional levels; furthermore, their cell-free counterparts might be involved intercellular communication. Non-coding considered to promising biomarker candidates early diagnosis as well therapeutic agents. This review aims provide clarity amidst vast body literature focusing diverse species ncRNAs, exploring structure, origin, function, applications miRNAs, siRNAs, lncRNAs, circRNAs, snRNAs, snoRNAs, eRNAs, paRNAs, YRNAs, vtRNAs, piRNAs. discuss molecular methods used detection functional studies vitro vivo. also address challenges must overcome enter new era therapy will reshape

Language: Английский

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Whole-transcriptome sequencing in neural and non-neural tissues of a mouse model identifies miR-34a as a key regulator in SMA pathogenesis

Molecular Therapy — Nucleic Acids, Journal Year: 2025, Volume and Issue: 36(2), P. 102490 - 102490

Published: Feb. 20, 2025

Spinal muscular atrophy (SMA) is a severe neurodegenerative disorder caused by deficiency of survival motor neuron (SMN). While significant progress has been made in SMA therapy rescuing SMN expression, limited knowledge about downstream genes hindered the development alternative therapies. Here, we conducted whole-transcriptome sequencing spinal cord, heart, and liver tissues mouse model at early postnatal ages to explore critical coding non-coding RNAs (ncRNAs). A large number differentially expressed (DE-RNAs) were obtained, including 2,771 mRNAs, 382 microRNAs (miRNAs), 1,633 long ncRNAs, 1,519 circular RNAs. Through in-depth data mining, unveiled deregulation miR-34a all tissues. Analysis competitive endogenous RNA networks DE-RNAs identified multiple novel targets Spag5 mRNA, lncRNA00138536, circRNA007386. Further vitro studies using myoblast human cardiomyocyte cell lines showed that knockdown upregulated miR-34a-5p overexpression alone disrupted cell-cycle progression through regulating its targets, recapitulating gene expression patterns observed cardiac tissue mice. Our results miRNA involved pathology, which sheds insights into molecular basis widespread abnormalities forms SMA.

Language: Английский

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Non-Coding RNAs as Prognostic Markers for Endometrial Cancer

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(6), P. 3151 - 3151

Published: March 19, 2021

Endometrial cancer (EC) has been classified over the years, for prognostic and therapeutic purposes. In recent classification systems have emerging not only based on EC clinical pathological characteristics but also its genetic epigenetic features. Noncoding RNAs (ncRNAs) are as promising markers in several types, including EC, which their value is currently under investigation will likely integrate present tools protein coding genes. This review aims to underline importance of events tumorigenesis, by expounding upon role ncRNAs.

Language: Английский

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