Optimizing drug discovery: Surface plasmon resonance techniques and their multifaceted applications

Chemical Physics Impact, Journal Year: 2023, Volume and Issue: 8, P. 100414 - 100414

Published: Dec. 5, 2023

In recent years, researchers have made significant contributions to the field of Surface Plasmon Resonance sensors and their potential applications. SPR features include real-time monitoring biomolecular interactions, label-free detection, high sensitivity, ability analyze multiple samples at same time. These characteristics make biosensors a valuable tool in pharmaceutical industry for ensuring drug efficacy safety. Furthermore, reputation providing quantitative qualitative superior performance applications such as development, environmental monitoring, food safety, medical diagnostics, clinical diagnosis, biological investigations, biomolecule interactions. This review will go over advances how they can be used discovery development. We also look challenges opportunities using industry.

Language: Английский

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Solid phase synthesis of molecularly imprinted polymers for analytical and life science applications

TrAC Trends in Analytical Chemistry, Journal Year: 2025, Volume and Issue: 184, P. 118134 - 118134

Published: Jan. 6, 2025

Language: Английский

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Exploiting the design of surface plasmon resonance interfaces for better diagnostics: A perspective review

Talanta, Journal Year: 2023, Volume and Issue: 266, P. 125033 - 125033

Published: Aug. 5, 2023

Surface Plasmon Resonance based-sensors are promising tools for precision diagnostics as they can provide tests useful early and, whenever possible, non-invasive disease detection and monitoring. The design of novel, robust effective interfaces enabling the sensing a variety molecular interactions in highly selective sensitive manner is necessary step to obtain both accurate reliable by SPR. This review covers recent research efforts this area, specifically emphasizing well-designed applications real-life samples. In particular, after short introduction which identifies some critical challenges, emerging strategies integration linker, metal substrate recognition element on interface will be explored discussed three sections, well opportunities building SPR biosensors, easy use, with excellent sensitivities. Finally, summary more latest diagnostic provided, presenting new window into near-future perspectives.

Language: Английский

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Computationally Designed Epitope-Mediated Imprinted Polymers versus Conventional Epitope Imprints for the Detection of Human Adenovirus in Water and Human Serum Samples

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(4), P. 1831 - 1841

Published: March 15, 2024

Detection of pathogenic viruses for point-of-care applications has attracted great attention since the COVID-19 pandemic. Current virus diagnostic tools are laborious and expensive, while requiring medically trained staff. Although user-friendly cost-effective biosensors utilized detection, many them rely on recognition elements that suffer major drawbacks. Herein, computationally designed epitope-imprinted polymers (eIPs) conjugated with a portable piezoelectric sensing platform to establish sensitive robust biosensor human adenovirus (HAdV). The template epitope is selected from knob part HAdV capsid, ensuring surface accessibility. Computational simulations performed evaluate conformational stability epitope. Further, molecular dynamics executed investigate interactions between different functional monomers smart design eIPs. imprinted via solid-phase synthesis method produce eIPs using in silico-selected ingredients. synthetic receptors show remarkable detection sensitivity (LOD: 102 pfu mL–1) affinity (dissociation constant (Kd): 6.48 × 10–12 M) HAdV. Moreover, computational lead around twofold improved binding behavior than synthesized well-established conventional recipe. proposed strategy holds enormous potential intelligent ultrasensitive polymer binders.

Language: Английский

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Evaluation of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using a high figure-of-merit plasmonic multimode refractive index optical sensor

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Oct. 26, 2024

In recent years, following the outbreak of COVID-19 pandemic, there has been a significant increase in cases SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) and related deaths worldwide. Despite pandemic nearing its end due to introduction mass-produced vaccines against SARS-CoV-2, early detection diagnosis virus remain crucial preventing disease progression. This article explores rapid identification by implementing multimode plasmonic refractive index (MMRI) optical sensor, developed based on split ring resonator (SRR) design. The Finite Difference Time Domain (FDTD) numerical solution method simulates sensor. studied sensor demonstrates three resonance modes within reflection spectrum ranging from 800 nm 1400 nm. Its material composition dimensional parameters are optimized enhance sensor's performance. research indicates that all exhibit strong performance with high sensitivity figures merit. Notably, first mode achieves an exceptional 557 nm/RIU, while third exhibits commendable 453 nm/RIU Figure Merit (FOM) 45 RIU

Language: Английский

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Rapid Point-of-Care Assay by SERS Detection of SARS-CoV-2 Virus and Its Variants

Analytical Chemistry, Journal Year: 2022, Volume and Issue: 94(51), P. 17795 - 17802

Published: Dec. 13, 2022

Addressing the spread of coronavirus disease 2019 (COVID-19) has highlighted need for rapid, accurate, and low-cost diagnostic methods that detect specific antigens SARS-CoV-2 infection. Tests COVID-19 are based on reverse transcription PCR (RT-PCR), which requires laboratory services is time-consuming. Here, by targeting spike protein, we present a point-of-care SERS detection platform specifically detects antigen in one step captureing substrates probes aptamer-specific recognition. Using pseudovirus, without any pretreatment, virus its variants were detected handheld Raman spectrometer within 5 min. The limit (LoD) pseudovirus was 124 TU μL-1 (18 fM protein), with linear range 250-10,000 μL-1. Moreover, this assay can recognize cross reacting other coronaviruses or influenza A. Therefore, great potential application rapid assays SARS-CoV-2.

Language: Английский

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A Novel NanoMIP–SPR Sensor for the Point-of-Care Diagnosis of Breast Cancer

Micromachines, Journal Year: 2023, Volume and Issue: 14(5), P. 1086 - 1086

Published: May 21, 2023

Simple, fast, selective, and reliable detection of human epidermal growth factor receptor 2 (HER2) is utmost importance in the early diagnosis breast cancer to prevent its high prevalence mortality. Molecularly imprinted polymers (MIPs), also known as artificial antibodies, have recently been used a specific tool therapy. In this study, miniaturized surface plasmon resonance (SPR)-based sensor was developed using epitope-mediated HER2-nanoMIPs. The nanoMIP receptors were characterized dynamic light scattering (DLS), zeta potential, Fourier-transform infrared spectroscopy (FT-IR), transmission electron microscopy (TEM), energy-dispersive X-ray (EDX), fluorescent microscopy. average size nanoMIPs determined be 67.5 ± 12.5 nm. proposed novel SPR provided superior selectivity HER2 with limit (LOD) 11.6 pg mL-1 serum. specificity confirmed by cross-reactivity studies P53, serum albumin (HSA), transferrin, glucose. preparation steps successfully employing cyclic square wave voltammetry. nanoMIP-SPR demonstrates great potential for use robust sensitivity, selectivity, specificity.

Language: Английский

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Recent Advances in Molecularly Imprinted Polymers and Their Disease-Related Applications

Polymers, Journal Year: 2023, Volume and Issue: 15(21), P. 4199 - 4199

Published: Oct. 24, 2023

Molecularly imprinted polymers (MIPs) and the imprinting technique provide polymeric material with recognition elements similar to natural antibodies. The template of choice (i.e., antigen) can be almost any type smaller or larger molecule, protein, even tissue. There are various formats MIPs developed for different medical purposes, such as targeting, imaging, assay diagnostics, biomarker detection. Biologically applied widely used currently applications, targeting antigen also help in personalized medicine. synthetic sites tailor-made function analytics, drug delivery systems. This review will cover promising clinical applications MIP systems recently disease diagnosis treatment.

Language: Английский

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Boosting Electrochemical Sensing Performances Using Molecularly Imprinted Nanoparticles

Biosensors, Journal Year: 2024, Volume and Issue: 14(7), P. 358 - 358

Published: July 22, 2024

Nanoparticles of molecularly imprinted polymers (nanoMIPs) combine the excellent recognition ability with specific properties related to nanosize, such as a high surface-to-volume ratio, resulting in highly performing elements surface-exposed binding sites that promote interaction target and, turn, kinetics. Different synthetic strategies are currently available produce nanoMIPs, possibility select conditions relation nature monomers/templates importantly, tune nanoparticle size. The sensing properties, combined size, tunability, and flexibility protocols applicable different targets, have enabled widespread use nanoMIPs several applications, including sensors, imaging, drug delivery. present review summarizes applications specifically focusing on electrochemical detection, for which been mostly applied. After general survey most widely adopted nanoMIP approaches, integration nanoparticles transducers will be discussed, representing key step enabling reliable stable sensor response. mechanisms signal generation also compared, followed by an illustration nanoMIP-based employment application fields. potentialities sensors presented, possible reasons still limit their commercialization issues resolved coupling increasingly daily-use technology discussed.

Language: Английский

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Validation of a plasmonic-based serology biosensor for veterinary diagnosis of COVID-19 in domestic animals

Talanta, Journal Year: 2024, Volume and Issue: 271, P. 125685 - 125685

Published: Jan. 16, 2024

The coronavirus disease 2019 (COVID-19) pandemic recently demonstrated the devastating impact on public health, economy, and social development of zoonotic infectious diseases, whereby viruses jump from animals to infect humans. Due this potential cross species barrier, surveillance pathogens circulation in domestic close-to-human is indispensable, as they could be reservoirs. Optical biosensors, mainly those based Surface Plasmon Resonance (SPR), have widely its ability for providing direct, label-free, quantitative bioanalysis with excellent sensitivity reliability. This biosensor technology can provide a powerful tool veterinary field, potentially being helpful monitoring infection spread. We implemented multi-target COVID-19 serology plasmonic rapid testing screening common European animals. serological assay enables detection total SARS-CoV-2 antibodies (IgG + IgM) generated towards both S N viral antigens. analysis performed less than 15 min low-volume serum sample (<20 μL, 1:10 dilution), reaching limit 49.6 ng mL

Language: Английский

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