Preliminary Study on Sensor-Based Detection of an Adherent Cell’s Pre-Detachment Moment in a MPWM Microfluidic Extraction System

Sensors, Journal Year: 2025, Volume and Issue: 25(9), P. 2726 - 2726

Published: April 25, 2025

The extraction of adherent cells, such as B16 murine melanoma from Petri dish cultures is critical in biomedical applications, including cell reprogramming, transplantation, and regenerative medicine. Traditional detachment methods-enzymatic, mechanical, or chemical-often compromise viability by altering membrane integrity disrupting adhesion proteins. To address these challenges, this study investigated sensor-based detection the pre-detachment phase a MPWM (Microfluidic Pulse Width Modulation) system. Our approach integrates micromechatronic system with microfluidic suction circuit, real-time CCD imaging, computational analysis to detect characterize moment before full extraction. A precisely controlled hydrodynamic force field progressively disrupts multiple stages, reducing mechanical stress preserving integrity. Real-time video enables continuous monitoring positional dynamics oscillatory responses. Image processing deep learning algorithms determine object center coordinates, allowing dynamically adjust parameters. This optimizes while minimizing liquid absorption reflux volume, ensuring efficient By combining microfluidics, sensor detection, AI-driven image processing, established non-invasive method for optimizing detachment. These findings have significant implications single-cell research, medicine, high-throughput biotechnology, maximal minimal perturbation.

Language: Английский

Conducting Polymers-Based Gas Sensors: Principles, Materials, and Applications

Sensors, Journal Year: 2025, Volume and Issue: 25(9), P. 2724 - 2724

Published: April 25, 2025

Conducting polymers (CPs) have emerged as promising materials for gas sensors due to their organic nature coupled with unique and versatile optical, electrical, chemical, electrochemical properties. This review provides a comprehensive overview of the latest developments in conducting polymer-based sensors. First, fundamental sensing mechanisms CPs-based are elucidated, covering diverse transduction modes including electrochemical, chemiresistive, piezoelectric, field-effect transistor-based sensing. Next, various types employed sensors, such polypyrrole, polyaniline, polythiophene, composites introduced, emphasis on synthesis methods, structural characteristics, response Finally, wide range applications these is discussed, spanning industrial process control, environmental monitoring, food safety, biomedical diagnosis, other fields, well existing issues long-term stability humidity interference, summary biocompatibility regulatory standards conductive provided. By integrating insights from mechanisms, materials, applications, this offers holistic understanding It also highlights future research directions, device miniaturization, AI-assisted identification, multifunctional integrated systems, wearable flexible sensor platforms, enhanced sensitivity, selectivity, on-site detection capabilities.

Language: Английский