T Cell Response in Tuberculosis-Infected Patients Vaccinated against COVID-19 DOI Creative Commons
Luiz Henrique Agra Cavalcante‐Silva,

Ericka Garcia Leite,

Fernanda Silva Almeida

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(11), P. 2810 - 2810

Published: Nov. 19, 2023

Many studies have focused on SARS-CoV-2 and Mycobacterium tuberculosis (Mtb) co-infection consequences. However, after a vaccination plan against COVID-19, the cases of severe disease death are consistently controlled, although asymptomatic mild COVID-19 still happen together with (TB) cases. Thus, in this context, we sought to compare T cell response COVID-19-non-vaccinated -vaccinated patients active exposed antigens. Flow cytometry was used analyze activation markers (i.e., CD69 CD137) cytokines (IFN-γ, TNFα, IL-17, IL-10) levels CD4+ CD8+ cells upon exposure peptides. The data obtained showed that from non-vaccinated TB present high frequency TNF-α viral challenge compared vaccinated donors. Conversely, show IL-10 spike peptide stimulus patients. No differences were observed other parameters analyzed. results suggest reduced immune balance coinfected individuals may consequences for pathogen control, necessitating further research understand its impact clinical outcomes those concurrent Mtb infections.

Language: Английский

COVID-19 Biomarkers at the Crossroad between Patient Stratification and Targeted Therapy: The Role of Validated and Proposed Parameters DOI Open Access
Manuela Rizzi, Davide D’Onghia, Stelvio Tonello

et al.

International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(8), P. 7099 - 7099

Published: April 12, 2023

Clinical knowledge about SARS-CoV-2 infection mechanisms and COVID-19 pathophysiology have enormously increased during the pandemic. Nevertheless, because of great heterogeneity disease manifestations, a precise patient stratification at admission is still difficult, thus rendering rational allocation limited medical resources as well tailored therapeutic approach challenging. To date, many hematologic biomarkers been validated to support early triage SARS-CoV-2-positive patients monitor their progression. Among them, some indices proven be not only predictive parameters, but also direct or indirect pharmacological targets, allowing for more single-patient symptoms, especially in those with severe progressive disease. While blood test-derived parameters quickly entered routine clinical practice, other circulating proposed by several researchers who investigated reliability specific cohorts. Despite usefulness contexts potential interest such experimental markers implemented mainly due higher costs low availability general hospital settings. This narrative review will present an overview most commonly adopted practice promising ones emerging from population studies. Considering that each reflects aspect evolution, embedding new highly informative into testing could help stratification, guiding timely method intervention.

Language: Английский

Citations

15
Successful salvage of a severe COVID-19 patient previously with lung cancer and radiation pneumonitis by mesenchymal stem cells: a case report and literature review DOI Creative Commons
Xiaohua Huang,

Xin Tan,

Xiuwen Xie

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 6, 2024

During the COVID-19 pandemic, elderly patients with underlying condition, such as tumors, had poor prognoses after progressing to severe pneumonia and often response standard treatment. Mesenchymal stem cells (MSCs) may be a promising treatment for pneumonia, but MSCs are rarely used carcinoma. Here, we reported 67-year-old female patient lung adenocarcinoma who underwent osimertinib radiotherapy suffered from radiation pneumonitis. Unfortunately, she contracted that rapidly progressed pneumonia. She responded poorly frontline was in danger. Subsequently, received salvage four doses of MSCs, her symptoms surprisingly improved quickly. After CT scan presented significantly infection, discharged eventually. Her primary disease stable 6 months follow-up, no tumor recurrence or progression observed. an effective hyperactive inflammation due their ability related immunomodulation tissue repair. Our case suggests potential value is unresponsive conventional therapy infection. However, unless situation urgent, it needs considered caution tumors. The safety still

Language: Английский

Citations

1
Modulation of Paracellular Permeability in SARS-CoV-2 Blood-to-Brain Transcytosis DOI Creative Commons
Taylor E. Martinez, Karthick Mayilsamy, Shyam S. Mohapatra

et al.

Viruses, Journal Year: 2024, Volume and Issue: 16(5), P. 785 - 785

Published: May 15, 2024

SARS-CoV-2 primarily infects the lungs via ACE2 receptor but also other organs including kidneys, gastrointestinal tract, heart, and skin. brain, hematogenous route of viral entry to brain is still not fully characterized. Understanding how traverses blood-brain barrier (BBB) as well it affects molecular functions BBB are unclear. In this study, we investigated roles receptors DPP4 in infection discrete cellular components a transwell model comprising HUVECs, astrocytes, pericytes. Our results demonstrate that direct on does modulate paracellular permeability. Also, our show utilizes clathrin caveolin-mediated endocytosis traverse BBB, resulting side with minimal endothelial infection. conclusion, susceptible multiple ways, endothelium, pericytes involving and/or blood-to-brain transcytosis, which an event require presence host receptors.

Language: Английский

Citations

0
The kynurenine pathway and role in mental health of post-COVID-19 patients DOI
Melike Küçükkarapınar, Filiz Karadağ, Selçuk Candansayar

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 393 - 406

Published: Jan. 1, 2024

Language: Английский

Citations

0
Potential therapeutic biomolecules of hymenopteran venom against SARS-CoV-2 from Egyptian patients DOI Creative Commons

Eman A. Abd El Maksoud,

Magda H. Rady,

Ahmed Gad Taha Mahmoud

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 4, 2024

Abstract The therapeutic potential of insect-derived bioactive molecules as anti-SARS-CoV-2 agents has shown promising results. Hymenopteran venoms, notably from Apis mellifera (honeybee) and Vespa orientalis (oriental wasp), were examined for the first time in an vitro setting their anti-COVID-19 activity. This assessment utilized immunodiagnostic system to detect SARS-CoV-2 nucleocapsid antigen titer reduction. Further analyses, including cytotoxicity assays, plaque reduction silico docking-based screening, performed evaluate efficacy most potent venom. Results indicated that bee wasp venoms contain with effects against SARS-CoV-2.Nevertheless, venom exhibited superior compared venom, achieving a 90% maximal (EC 90 ) concentration effect depletion at 0.184 mg/mL, contrast 2.23 mg/mL was assessed on Vero E6 cells 48 h post-treatment using MTT assay. CC 50 cell growth 0.16617 cells. assay revealed 50% inhibition (IC 0.208 concentration. viral count 2.5 × 10 4 PFU/mL initial 5 PFU/mL. In data strong attraction binding sites ACE2 protein, indicating ideal interactions. substantiates inhibitor SARS-CoV-2, suggesting its consideration prospective natural preventive curative antiviral drug. conclusion, hymenopteran particularly hold promise source biomolecules SARS-CoV-2. More research clinical trials are needed these results investigate translation into innovative therapies.

Language: Английский

Citations

0
T Cell Response in Tuberculosis-Infected Patients Vaccinated against COVID-19 DOI Creative Commons
Luiz Henrique Agra Cavalcante‐Silva,

Ericka Garcia Leite,

Fernanda Silva Almeida

et al.

Microorganisms, Journal Year: 2023, Volume and Issue: 11(11), P. 2810 - 2810

Published: Nov. 19, 2023

Many studies have focused on SARS-CoV-2 and Mycobacterium tuberculosis (Mtb) co-infection consequences. However, after a vaccination plan against COVID-19, the cases of severe disease death are consistently controlled, although asymptomatic mild COVID-19 still happen together with (TB) cases. Thus, in this context, we sought to compare T cell response COVID-19-non-vaccinated -vaccinated patients active exposed antigens. Flow cytometry was used analyze activation markers (i.e., CD69 CD137) cytokines (IFN-γ, TNFα, IL-17, IL-10) levels CD4+ CD8+ cells upon exposure peptides. The data obtained showed that from non-vaccinated TB present high frequency TNF-α viral challenge compared vaccinated donors. Conversely, show IL-10 spike peptide stimulus patients. No differences were observed other parameters analyzed. results suggest reduced immune balance coinfected individuals may consequences for pathogen control, necessitating further research understand its impact clinical outcomes those concurrent Mtb infections.

Language: Английский

Citations

1