Oxidative Homeostasis in Follicular Fluid and Embryo Quality—A Pilot Study

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 388 - 388

Published: Jan. 4, 2025

The objective of this study was to measure the different redox biomarker levels within follicular fluid (FF) and evaluate correlations with embryo quality using one follicle–one oocyte/embryo approach. prospective included 54 women (average age 34.6 ± 3.0 years). Out 235 mature metaphase II cells that underwent intracytoplasmic sperm injection, fertilization achieved in 177 cells, producing 92 Grade I embryos, 26 39 III 20 IV embryos. activities antioxidant enzymes, superoxide dismutase, glutathione peroxidase, transferase were significantly higher group consisting lower-quality (Grades II–IV) embryos comparison top-quality (Grade I) (p = 0.011; p 0.021; 0.008, respectively). concentration oxidative stress markers, malondialdehyde, 8-hydroxy-2′-deoxyguanosine, thiol groups increased compared (0.027; 0.018; 0.021, Furthermore, a significant positive correlation between each marker enzymes observed < 0.001). According our findings, best and, consequently, better vitro outcomes are linked low enzyme activity.

Language: Английский

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Oxidative Stress and the NLRP3 Inflammasome: Focus on Female Fertility and Reproductive Health

Cells, Journal Year: 2025, Volume and Issue: 14(1), P. 36 - 36

Published: Jan. 2, 2025

Chronic inflammation is increasingly recognized as a critical factor in female reproductive health; influencing natural conception and the outcomes of assisted technologies such vitro fertilization (IVF). An essential component innate immunity, NLR family pyrin domain-containing 3 (NLRP3) inflammasome one major mediators inflammatory responses, its activation closely linked to oxidative stress. This interaction contributes decline oocyte quality, reduced potential, impaired embryo development. In ovarian milieu, stress NLRP3 interact intricately, their combined effects on competence are significant. The aims this review examine these molecular mechanisms explore therapeutic strategies targeting activity, with goal enhancing fertility improving clinical health.

Language: Английский

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The Role of Oxidative Stress in Infertility

Journal of Personalized Medicine, Journal Year: 2023, Volume and Issue: 13(8), P. 1264 - 1264

Published: Aug. 15, 2023

(1) Background: Oxidative stress markers in the follicular fluid and serum of patient with IVF results (number fertilized oocytes, number embryos, cumulative pregnancy rate) are important establishing causes infertility. (2) Methods: 42 patients were enrolled study over duration 24 months divided into two groups: group female etiology for infertility as a tubal factor, diminished ovarian reserve, endometriosis, unexplained infertility, control consisting male excluding sperm donors no factor cause. On day puncture IVF, collected from patients. (3) Results: Malondialdehyde was higher group. The total antioxidant capacity is positively correlated rate. There statistically significant difference oxidative both groups. (4) Conclusions: environment to contain reactive oxygen species (ROS) leads rate

Language: Английский

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Oxidative stress affects sperm health and fertility—Time to apply facts learned at the bench to help the patient: Lessons for busy clinicians

Reproductive Medicine and Biology, Journal Year: 2024, Volume and Issue: 23(1)

Published: Jan. 1, 2024

Abstract Background Increased oxidative stress (OS), resulting from the delicate balance between reactive oxygen species (ROS) production and antioxidant defense, is closely linked to sperm abnormalities male subfertility. Elevated ROS levels particularly affect quality. The vulnerability of spermatozoa due absence DNA repair mechanisms high presence polyunsaturated fatty acids in their membranes. Methods This article updates advances our understanding molecular damage caused by OS spermatozoa, including lipid peroxidation, damage, motility, functionality. Additionally, review discusses challenges diagnosing semen recommends accurate sensitive testing methods. Case studies are utilized demonstrate effective management infertility OS. Main findings Highlighting need bridge gap research clinical practice, this suggests strategies for clinicians, such as lifestyle dietary changes therapies. emphasizes modifications personalized care managing Conclusion calls early detection intervention interdisciplinary collaboration improve patient cases related increased

Language: Английский

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A Retrospective Cohort Study Investigating the Effect of Intraovarian Platelet-Rich Plasma Therapy on the Oxidative State of Follicular Fluid in Women with Diminished Ovarian Reserve

Chonnam Medical Journal, Journal Year: 2025, Volume and Issue: 61(1), P. 46 - 46

Published: Jan. 1, 2025

This study hypothesized that oxidative stress in women with diminished ovarian reserve (DOR) can influence vitro fertilization (IVF) outcomes. Therefore, we investigated the effect of intraovarian platelet-rich plasma (PRP) administration on state follicular fluid DOR patients. Following follow-up a retrospective cohort study, was isolated from twenty-five The total antioxidant capacity (TAC), catalase (CAT), and superoxide dismutase (SOD) activities, oxidant status (TOS), malondialdehyde (MDA) levels were measured before after PRP using colorimetric methods. estradiol (E2), anti-mullerian hormone (AMH), luteinizing (LH), follicle-stimulating (FSH) at baseline two menstrual cycles. quantity quality oocytes embryos assessed both stages. injection, an increase TAC levels, elevated CAT SOD decline TOS MDA content observed. AMH E2 also increased post-treatment. Additionally, relative number observed single injection. Intraovarian injection improves potentially enhances IVF outcomes women.

Language: Английский

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Impact of combining laparoscopy with traditional Chinese medicine on oxidative stress in endometriosis-related infertility: A systematic review and meta-analysis

Medicine, Journal Year: 2025, Volume and Issue: 104(15), P. e41692 - e41692

Published: April 11, 2025

Background: To evaluate the effect of laparoscopic procedures integrated with traditional Chinese medicine (TCM), specifically aimed at enhancing blood flow and alleviating stasis, on oxidative stress levels in individuals endometriosis-induced infertility. Methods: We performed a systematic quantitative review to effects surgery combined TCM endometriosis-related infertility by circulation resolving stasis. A literature search 9 major databases was performed. Meta-analysis using Review Manager version 5.4 (R Foundation for Statistical Computing, Vienna, Austria) Stata Release 16.0 (StataCorp LLC, College Station, TX). This registered International Prospective Register Systematic Reviews (i.e., “PROSPERO”) (registration number: CRD42024526800). Results: Thirteen trials comprising 1084 participants were included. Laparoscopy promoting removing stasis significantly reduced 8-isoprostane prostaglandin F2α (mean difference [MD] −29.57 [95% confidence interval (CI) −32.65 −26.49]; P < .001), regulated activation, normal T cell expressed secreted (MD −231.83 CI −341.33 −122.32]; reactive oxygen species −0.92; −1.12 −0.73]; monocyte chemoattractant protein-1 −31.23 −42.70 −19.77]; increased glutathione peroxidase 31.45 26.04 36.87]; vitamin E 4.86 3.77 5.94]; superoxide dismutase (standardized MD 0.92 0.41 1.42]; .001). Conclusion: Compared alone, combination demonstrated potential ameliorate patients However, further large-scale clinical are required confirm these findings.

Language: Английский

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From infection to infertility: a review of the role of human papillomavirus-induced oxidative stress on reproductive health and infertility

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 28, 2025

Infertility has emerged as a significant global health concern, affecting nearby 8-12% of couples in reproductive age worldwide. Increasing evidence suggests potential link between human papillomavirus (HPV) and infertility both men women. Some research indicate that HPV can infect various components semen, potentially sperm quality by decreasing motility, viability, increasing DNA fragmentation, all which may contribute to male infertility. The virus attach the equatorial region head, enabling infected transmit oocyte or placenta. Consequently, induces apoptosis trophoblastic cells disrupts their adhesion endometrial cells, raises risk miscarriage. also affect ovarian reserve causing chronic inflammation, impair granulosa cell function lower serum anti-Müllerian hormone (AMH) levels. Besides, HPV-related immune responses producing anti-sperm antibodies (ASAs), cause clumping, reduce motility through cervical mucus, activate complement system damages female tract interfere with sperm-egg interactions. Moreover, infection been linked reduced success rates assisted technologies (ART), disrupting critical processes such acrosome reaction, sperm-oocyte interaction, fusion. One mechanism contributes is oxidative stress (OS). Triggered OS negatively impact damage system, ultimately contributing Despite these associations, precise mechanisms strength relationship remain uncertain. Thus, this review seeks investigate on infertility, particularly its effects OS. A clearer understanding could inform future strategies for addressing

Language: Английский

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Advancing Assisted Reproduction Technology: The Role of Nanotechnology in Enhancing Fertility Treatments and Diagnostics

Journal of Pharmacy And Bioallied Sciences, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

A BSTRACT Nanotechnology, which utilizes synthetic chemistry to alter atoms and create imaging platforms with dense computer circuitry, helps magnify small objects. Assisted reproduction technology (ART) is a branch of medical science that enables couples unable conceive naturally have children. This aids in conception restores fertility, addressing the challenges natural reproduction. Nanotechnology can significantly enhance ART practices, become crucial modern medicine for diagnosing treating fertility-related issues. Despite various research technology, nanotechnology offers solutions previously encountered problems. Recent studies show incorporating into new technologies improves accuracy selectivity, leading more efficient effective outcomes. Several clinical trials focus on early diagnosis genital infections, malignancies, cellular therapies. However, developing these treatments requires stronger collaboration between pharmaceutics, chemistry, molecular sciences transition from practical application. Forming alliances an understanding biological processes ability navigate obstacles help overcome regulatory technical barriers. Such collaborations would enable precise assessments nanotechnology-based equipment techniques. study emphasizes current applications nanotechnology, highlighting its significant potential improving enabling rapid reproductive tract infections ailments.

Language: Английский

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Micronutrient–Antioxidant Therapy and Male Fertility Improvement During ART Cycles

Nutrients, Journal Year: 2025, Volume and Issue: 17(2), P. 324 - 324

Published: Jan. 17, 2025

Today, accumulating evidence highlights the impact of oxidative stress (OS) on semen quality. It is considered to be a key factor contributing decline in male fertility. OS detected 30-80% men with infertility, highlighting its strong association impaired reproductive function and clinical outcomes following use assisted technologies. Spermatozoa are particularly vulnerable damage due their high content polyunsaturated fatty acids (PUFAs) limited antioxidant defense abilities. arises from an imbalance between production reactive oxygen species capacity neutralize or repair adverse effects. Evidence indicates that leads lipid peroxidation, protein oxidation, mitochondrial dysfunction, genomic instability. Micronutrient-antioxidant therapies can play role infertility improvement by neutralizing free radicals preventing cellular damage. Many different micronutrients, including L-carnitine, L-glutathione, coenzyme Q10, selenium, zinc, as well vitamins complexes, proposed improve sperm parameters fertility potential. This study aims review supplementation parameters, volume, motility, concentration, morphology, genome integrity (maturity fragmentation), vitro fertilization (IVF) outcomes. Antioxidant intake balanced lifestyle reduce enhancing spermatogenesis spermiogenesis processes, improving quality, protecting DNA integrity.

Language: Английский

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Redox profiling of preovulatory follicular fluid in the donkey is species-specific, and contributes to modulate sperm function

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 23, 2025

Language: Английский

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