Revealing differential expression patterns of piRNA in FACS blood cells of SARS-CoV−2 infected patients DOI Creative Commons
К. А. Кондратов, Alexander A. Artamonov, Yu. V. Nikitin

et al.

BMC Medical Genomics, Journal Year: 2024, Volume and Issue: 17(1)

Published: Aug. 14, 2024

Non-coding RNA expression has shown to have cell type-specificity. The regulatory characteristics of these molecules are impacted by changes in their levels. We performed next-generation sequencing and examined small RNA-seq data obtained from 6 different types blood cells separated fluorescence-activated sorting severe COVID−19 patients healthy control donors. In addition examining the behavior piRNA SARS-CoV−2 infected patients, our aim was present a distinct differential portrait for each separate type. observed that depending on type cell, sorted (erythrocytes, monocytes, lymphocytes, eosinophils, basophils, neutrophils) altering patterns. After analyzing piRNAs set with COVID−19, we 3 significantly elevated - piR−33,123, piR−34,765, piR−43,768 9 downregulated erythrocytes. all 19 were upregulated. Monocytes presented larger amount statistically significant piRNA, 5 upregulated (piR−49039 piR−31623, piR−37213, piR−44721, piR−44720) 35 downregulated. It been previously piR−31,623 associated respiratory syncytial virus infection, taking account major role transposon silencing, presume patterns which could be signal indirect antiviral activity or specific state. Additionally,

Language: Английский

piRNA/PIWI Pathways and Epigenetic Crosstalk in Human Diseases: Molecular Insights into HIV-1 Infection and Drugs of Abuse DOI Creative Commons
Karthick Chennakesavan, James Haorah, Thangavel Samikkannu

et al.

Molecular Therapy — Nucleic Acids, Journal Year: 2025, Volume and Issue: 36(1), P. 102473 - 102473

Published: Feb. 1, 2025

P-element-induced wimpy (PIWI)-interacting RNAs (piRNAs) and PIWI proteins have long been studied in insects germline cells for their roles regulating transposable elements (TEs). However, emerging evidence suggests that piRNAs also play crucial human diseases beyond gametocyte protection, these molecules are implicated the onset progression of various diseases, particularly those arising somatic cells. Notably, increasingly recognized involvement cancers, cardiovascular neurodegenerative disorders, viral infections, including HIV. This review first provides an overview piRNAs/PIWIs interactions with TEs primary targets. We then explore molecular mechanisms signaling pathways through which PIWIs modulate disease processes, focusing on neurodegeneration, Special attention is given to role piRNA/PIWI complexes gene transcription, translation, post-translational modifications context disease. Additionally, we address research into HIV- drug abuse or substance abuse-associated highlighting existing knowledge gaps. Finally, discuss future directions understand better functions piRNAs/PIWI health

Language: Английский

Citations

0
The potential emerging role of piRNA/PIWI complex in virus infection DOI
Yanyan Li, Kai Wang, Wen Liu

et al.

Virus Genes, Journal Year: 2024, Volume and Issue: 60(4), P. 333 - 346

Published: June 4, 2024

Language: Английский

Citations

3
Revealing differential expression patterns of piRNA in FACS blood cells of SARS-CoV−2 infected patients DOI Creative Commons
К. А. Кондратов, Alexander A. Artamonov, Yu. V. Nikitin

et al.

BMC Medical Genomics, Journal Year: 2024, Volume and Issue: 17(1)

Published: Aug. 14, 2024

Non-coding RNA expression has shown to have cell type-specificity. The regulatory characteristics of these molecules are impacted by changes in their levels. We performed next-generation sequencing and examined small RNA-seq data obtained from 6 different types blood cells separated fluorescence-activated sorting severe COVID−19 patients healthy control donors. In addition examining the behavior piRNA SARS-CoV−2 infected patients, our aim was present a distinct differential portrait for each separate type. observed that depending on type cell, sorted (erythrocytes, monocytes, lymphocytes, eosinophils, basophils, neutrophils) altering patterns. After analyzing piRNAs set with COVID−19, we 3 significantly elevated - piR−33,123, piR−34,765, piR−43,768 9 downregulated erythrocytes. all 19 were upregulated. Monocytes presented larger amount statistically significant piRNA, 5 upregulated (piR−49039 piR−31623, piR−37213, piR−44721, piR−44720) 35 downregulated. It been previously piR−31,623 associated respiratory syncytial virus infection, taking account major role transposon silencing, presume patterns which could be signal indirect antiviral activity or specific state. Additionally,

Language: Английский

Citations

1