Regulating ferredoxin electron transfer using nanobody and antigen interactions DOI Creative Commons
Albert Truong, Jonathan J. Silberg

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Fission and fusion can be used to generate new regulatory functions in proteins. This approach has been create ferredoxins (Fd) whose cellular electron transfer is dependent upon small molecule binding. To investigate whether Fd fragments monitor macromolecular binding reactions, we investigated the effects of fusing Mastigocladus laminosus nanobodies their protein antigens. When arising from fission were fused green fluorescent (GFP) three different anti-GFP nanobodies, split proteins identified that supported Fd-mediated Fd-NADP reductase (FNR) sulfite (SIR) Escherichia coli . However, order nanobody antigen affected transfer. Insertion these within had differing on One domain-insertion variant was unable support unless it coexpressed with GFP, while others absence GFP. These findings show how Fds engineered so regulated by macromolecules, they reveal importance exploring homologs strategies when engineering biomolecular switches.

Language: Английский

Regulating ferredoxin electron transfer using nanobody and antigen interactions DOI Creative Commons
Albert Truong, Jonathan J. Silberg

RSC Chemical Biology, Journal Year: 2025, Volume and Issue: 6(5), P. 746 - 753

Published: Jan. 1, 2025

Fission and fusion can be used to generate new regulatory functions in proteins. This approach has been create ferredoxins (Fd) whose cellular electron transfer is dependent upon small molecule binding. To investigate whether Fd fragments monitor macromolecular binding reactions, we investigated the effects of fusing Mastigocladus laminosus single domain antibodies, also known as nanobodies, their protein antigens. When arising from fission were fused green fluorescent (GFP) three different anti-GFP split proteins identified that supported Fd-mediated Fd-NADP reductase (FNR) sulfite (SIR) Escherichia coli. However, order nanobody antigen affected transfer. Insertion these nanobodies within had differing on One domain-insertion variant was unable support unless it coexpressed with GFP, while others absence GFP. These findings show how Fds engineered so regulated by macromolecules, they reveal importance exploring homologs strategies when engineering biomolecular switches.

Language: Английский

Citations

0
Simultaneous removal of carbamazepine, nitrate, and copper in a biofilm reactor filled with FeMn-modified ceramsite DOI
Ying Zhang,

Miqi Ren,

Junfeng Su

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 491, P. 137871 - 137871

Published: March 9, 2025

Language: Английский

Citations

0
Fimsbactin Siderophores From a South African Marine Sponge Symbiont, Marinomonas sp. PE14‐40 DOI Creative Commons

Nompumelelo Philile Praiseworth Ikegwuoha,

Thea Hanekom,

Elzaan Booysen

et al.

Microbial Biotechnology, Journal Year: 2025, Volume and Issue: 18(5)

Published: May 1, 2025

ABSTRACT Low iron levels in marine habitats necessitate the production of structurally diverse siderophores by many bacterial species for acquisition. Siderophores exhibit bioactivities ranging from chelation reduction hemochromatosis sufferers to antimicrobial activity either their own right or when coupled known antibiotics targeted delivery molecular imaging. Thus, environments are a sought‐after resource novel that could have pharmaceutical industrial application. The fimsbactins A‐F ( 1–6 ) mixed catechol‐hydroxamate only been reported be produced Acinetobacter with fimsbactin biosynthetic gene clusters (BGCs) widespread among within this genus. Here, we identified putative BGC an uncharacterized isolate, Marinomonas sp. PE14‐40. Not was synteny not conserved comparing pathway PE14‐40 sp., but five core genes found canonical located elsewhere on genome and do form part cluster PE14‐40, four these, fbsBCDL, colocalized. Through ESI‐MS/MS analysis extracts analogues 1 6 were identified, as well two new analogues, 7 8 , containing previously unreported L‐lysine‐derived hydroxamate moiety, N ‐acetyl‐ ‐hydroxycadaverine. Feeding experiments using stable isotope‐label L‐lysine provided further evidence ‐hydroxycadaverine moiety . study demonstrates functional conservation seemingly disparate pathways enzyme promiscuity's role producing compounds.

Language: Английский

Citations

0
Regulating ferredoxin electron transfer using nanobody and antigen interactions DOI Creative Commons
Albert Truong, Jonathan J. Silberg

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Fission and fusion can be used to generate new regulatory functions in proteins. This approach has been create ferredoxins (Fd) whose cellular electron transfer is dependent upon small molecule binding. To investigate whether Fd fragments monitor macromolecular binding reactions, we investigated the effects of fusing Mastigocladus laminosus nanobodies their protein antigens. When arising from fission were fused green fluorescent (GFP) three different anti-GFP nanobodies, split proteins identified that supported Fd-mediated Fd-NADP reductase (FNR) sulfite (SIR) Escherichia coli . However, order nanobody antigen affected transfer. Insertion these within had differing on One domain-insertion variant was unable support unless it coexpressed with GFP, while others absence GFP. These findings show how Fds engineered so regulated by macromolecules, they reveal importance exploring homologs strategies when engineering biomolecular switches.

Language: Английский

Citations

0