The Cancer Chimera: Impact of Vimentin and Cytokeratin Co-Expression in Hybrid Epithelial/Mesenchymal Cancer Cells on Tumor Plasticity and Metastasis DOI Open Access
Nick A. Kuburich,

Julia M. Kiselka,

Petra den Hollander

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(24), P. 4158 - 4158

Published: Dec. 13, 2024

The epithelial–mesenchymal transition (EMT) program is critical to metastatic cancer progression. EMT results in the expression of mesenchymal proteins and enhances migratory invasive capabilities. In a small percentage cells, stemness-associated genes that provide advantage. Although had been viewed as binary event, it has recently become clear leads spectrum phenotypes, including hybrid epithelial/mesenchymal (E/M) cells have significantly greater capability than on epithelial or ends spectrum. As E/M are rarely observed physiological, non-diseased states adult human body, these potential biomarkers drug targets. Hybrid distinguished by co-expression proteins, such intermediate filament cytokeratin (CK; epithelial) vimentin (VIM; mesenchymal). filaments extensively used for pathological characterization detection aggressive carcinomas, little known regarding interactions between CK VIM when co-expressed cells. This review describes characteristics with focus unique CK. We will discuss structures functions two how they may interact Additionally, we what about cell-surface their predictive therapeutic

Language: Английский

Advances in Engineered Bacteria for Cancer Therapy DOI Creative Commons
Wenping Pan, Hongmei Liu, Decheng Wu

et al.

Precision medicine and engineering., Journal Year: 2025, Volume and Issue: unknown, P. 100017 - 100017

Published: Feb. 1, 2025

Language: Английский

Citations

0

Antitumor Efficacy of Interleukin 12-Transfected Mesenchymal Stem Cells in B16-F10 Mouse Melanoma Tumor Model DOI Creative Commons
Urška Kamenšek, Tim Božič, Maja Čemažar

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 278 - 278

Published: Feb. 20, 2025

Background/Objectives: Mesenchymal stromal cells (MSCs) hold the potential for tumor-targeted gene delivery due to their ex vivo manipulability, low immunogenicity, scalability, and inherent tumor-homing properties. Despite widespread use of viral vectors MSC genetic modification, safety concerns have prompted interest in non-viral alternatives, such as electrotransfer (GET). This study aimed optimize GET parameters MSCs transfection, assess biodistribution after administration, evaluate therapeutic interleukin-12 (IL-12)-modified a mouse melanoma model. Methods: Human were isolated from umbilical cords under ethically approved protocols. protocols optimized using fluorescent reporter transfection efficiency cell viability. was examined following intravenous intratumoral injections murine tumor models luminescent gene. The efficacy IL-12-modified assessed syngeneic Results: Optimized achieved 80% viability 90%. Biodistribution studies demonstrated effective retention injections, whereas administration resulted predominant localization lungs. injected intratumorally significantly inhibited growth, delaying progression by five days compared controls. Conclusions: conditions enabled high-efficiency, high-viability facilitating vehicles localized cytokine delivery. While innate tropism not conclusively demonstrated, highlights reliable platform underscores promise therapy.

Language: Английский

Citations

0

Clinical Trials of Cancer Immunogene Therapies in Companion Animals: An Update (2017–2024) DOI Creative Commons
Gerardo C. Glikin,

Liliana M.E. Finocchiaro

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 329 - 329

Published: April 3, 2025

This review summarizes the findings of veterinary clinical trials on immunogene therapy published between 2017 and 2024. Various tumor types, including melanoma (canine feline), mastocytoma (canine), mammary adenocarcinoma osteosarcoma sarcoid (equine), were treated using diverse strategies. Non-viral vectors predominantly used to deliver genes encoding tumor-associated antigens, cytokines, or suicide enzymes. Among these non-viral methods, electrotransfer was most commonly employed technique for introducing therapeutic into cells. Generally, procedures resulted in minimal no adverse side effects, animals often showed significant improvements, such as enhanced quality life, delayed suppressed recurrence metastasis, increased survival times. Some innovative approaches hold great potential adjunct therapies standard treatments. The promising outcomes from studies companion strongly support their application oncology provide valuable preclinical data (including safety assessments proof-of-concept studies) analogous human trials.

Language: Английский

Citations

0

CD4 T cells acquire innate capability upon classical T cell activation DOI Creative Commons
Nima Yassini, Eva Goljat,

Camilla Panetti

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Abstract Memory T cells, a sizable compartment of the mature immune system, enable enhanced responses upon re-infection with same pathogen. We have recently shown that virus-experienced innate acting (T IA ) cells can modulate infectious or autoimmune diseases through TCR-independent IFN-γ production. However, how these arise remains unclear. Here, we show CD4 are present in various disease settings hinting towards disease-agnostic nature. TCR stimulation and CD28 co-stimulation sufficient to induce naïve murine human become capable cytokine-mediated, responses. In true fashion, adoptive transfer vitro -induced mice yielded response during phase Legionella pneumophila infection. Our data thus shows more ubiquitous than anticipated could therefore be involved expected.

Language: Английский

Citations

0

The Cancer Chimera: Impact of Vimentin and Cytokeratin Co-Expression in Hybrid Epithelial/Mesenchymal Cancer Cells on Tumor Plasticity and Metastasis DOI Open Access
Nick A. Kuburich,

Julia M. Kiselka,

Petra den Hollander

et al.

Cancers, Journal Year: 2024, Volume and Issue: 16(24), P. 4158 - 4158

Published: Dec. 13, 2024

The epithelial–mesenchymal transition (EMT) program is critical to metastatic cancer progression. EMT results in the expression of mesenchymal proteins and enhances migratory invasive capabilities. In a small percentage cells, stemness-associated genes that provide advantage. Although had been viewed as binary event, it has recently become clear leads spectrum phenotypes, including hybrid epithelial/mesenchymal (E/M) cells have significantly greater capability than on epithelial or ends spectrum. As E/M are rarely observed physiological, non-diseased states adult human body, these potential biomarkers drug targets. Hybrid distinguished by co-expression proteins, such intermediate filament cytokeratin (CK; epithelial) vimentin (VIM; mesenchymal). filaments extensively used for pathological characterization detection aggressive carcinomas, little known regarding interactions between CK VIM when co-expressed cells. This review describes characteristics with focus unique CK. We will discuss structures functions two how they may interact Additionally, we what about cell-surface their predictive therapeutic

Language: Английский

Citations

0