Irisin restrains neuroinflammation in mouse experimental autoimmune encephalomyelitis via regulating microglia activation DOI Creative Commons
Qiuxia Zhang, Linjie Zhang, Ning Zhao

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 29, 2025

Multiple sclerosis is a chronic autoimmune demyelinating disorder predominantly affecting the white matter of central nervous system, with experimental encephalomyelitis (EAE) serving as its classical animal model. Irisin, glycosylated protein derived from proteolytic cleavage fibronectin type III domain-containing 5, plays significant role in metabolic regulation and inflammatory modulation within organism. In this study, we systematically investigated therapeutic effects underlying mechanism Irisin on EAE BV2 microglial cells through comprehensive methodologies including quantitative real-time polymerase chain reaction, immunofluorescence staining western blot. exerts neuroprotective mice, significantly ameliorating both clinical pathological manifestations disease. Mechanistically, attenuated response reduced number microglia NF-κBp65 signaling pathway. conclusion, these results collectively suggest that alleviates progression by suppressing activation via pathway, highlighting potential promising target for multiple treatment.

Language: Английский

Irisin restrains neuroinflammation in mouse experimental autoimmune encephalomyelitis via regulating microglia activation DOI Creative Commons
Qiuxia Zhang, Linjie Zhang, Ning Zhao

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 29, 2025

Multiple sclerosis is a chronic autoimmune demyelinating disorder predominantly affecting the white matter of central nervous system, with experimental encephalomyelitis (EAE) serving as its classical animal model. Irisin, glycosylated protein derived from proteolytic cleavage fibronectin type III domain-containing 5, plays significant role in metabolic regulation and inflammatory modulation within organism. In this study, we systematically investigated therapeutic effects underlying mechanism Irisin on EAE BV2 microglial cells through comprehensive methodologies including quantitative real-time polymerase chain reaction, immunofluorescence staining western blot. exerts neuroprotective mice, significantly ameliorating both clinical pathological manifestations disease. Mechanistically, attenuated response reduced number microglia NF-κBp65 signaling pathway. conclusion, these results collectively suggest that alleviates progression by suppressing activation via pathway, highlighting potential promising target for multiple treatment.

Language: Английский

Citations

0