Assessment of the Capability of Mesenchymal Stem Cells and/or Pyrroloquinoline Quinone in Compensating the Age-Related Dysfunctions of AMP-Activated Protein Kinase Pathway in Wistar Rats

Cell Biochemistry and Biophysics, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Language: Английский

Assessment of the Capability of Mesenchymal Stem Cells and/or Pyrroloquinoline Quinone in Compensating the Age-related Dysfunctions of AMP-Activated Protein Kinase Pathway in Wistar Rats

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 7, 2025

Aging is characterized by a decline in physiological functions and an increased susceptibility to age-related diseases. This study investigates the therapeutic potential of mesenchymal stem cells (MSCs) pyrroloquinoline quinone (PQQ), individually combination, counteract aging-related declines, with specific focus on their modulation AMP-activated protein kinase (AMPK) pathway, key regulator cellular energy homeostasis stress response. was induced thirty-seven female rats using D-galactose, simulating metabolic imbalances oxidative characteristic aging. The experimental groups included controls, aged without treatment, treated MSCs, PQQ, or combined MSC-PQQ regimen. MSC homing analyses Behavioral assessments, assays, gene expression profiling, histopathological evaluations were conducted provide multidimensional view treatment efficacy. confirmed successful tissue localization repair, underscoring regenerative capacity MSCs. Remarkably, therapy (APQQST) markedly improved anxiety-related behaviors, evidenced rearing grooming activities (p < 0.01). Oxidative biomarkers supported these findings; exhibited significantly reduced malondialdehyde (MDA) levels elevated antioxidant defenses, including glutathione (GSH) peroxidase (GPX) Gene analysis highlighted beneficial upregulation genes such as LKB1, PFKFB3, TSC2, HMGR, crucial for response, combination showing most pronounced effects. Furthermore, assessments underscored significant liver recovery groups, particularly (APQQST), minimal vacuolar degeneration restored hepatic architecture These findings highlight synergistic effects MSCs PQQ mitigating behavioral, molecular, aspects aging, promising agents promoting healthy aging offering foundation future translational research clinical applications.

Language: Английский

The Intraosseous Environment: Physiological Parameters, Regulatory Mechanisms, and Emerging Insights in Bone Biology

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3876 - 3876

Published: April 19, 2025

The intraosseous environment is a dynamic and intricate system integral to bone health, encompassing vascular, cellular, biochemical interactions that drive key processes such as hematopoiesis, remodeling, systemic mineral regulation. This review examines the structural composition of matrix, diverse cellular landscape, interconnected vascular nervous networks, highlighting their roles in preserving function responding pathological changes. Recent studies reveal regulatory mechanisms involving oxygen tension, ionic balance, signaling molecules, mechanotransduction pathways shape metabolism its adaptation mechanical forces. Insights into microenvironment's metabolic shifts cancer interaction with inflammation underscore pivotal role disease progression therapeutic innovation. Additionally, advances imaging techniques biomaterials fuel progress regeneration understanding microenvironment. Exploring physiochemical dynamics networks within unlocks potential clinical applications diseases, tissue engineering, disorders. comprehensive bridges fundamental science translational research, offering insights complex yet essential environment.

Language: Английский