Extracellular vesicles for ophthalmic and dermal ailments

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 283 - 345

Published: Jan. 1, 2025

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Synergistic Anti‐Aging Effects of Adipose‐Derived Stem Cell Extracellular Vesicles Loaded With Natural Compounds

Journal of Cosmetic Dermatology, Journal Year: 2025, Volume and Issue: 24(2)

Published: Feb. 1, 2025

Small extracellular vesicles from adipose-derived stem cells (ASC-sEVs) are gaining attentions rapidly for inherent therapeutic values in skin care and cosmetics. However, the optimal combinations of ASC-sEVs certain natural compounds synergistic anti-aging effects have not been systematically studied. Human were purified culture supernatant ASCs multi-omics datasets miRNAs, proteins lipids analyzed pathways regulating homeostasis. then loaded with nicotinamide riboside (NR), resveratrol (RES), vitamin C (VITC), retinol (RET) arbutin (ARB) at different concentrations by sonication-incubation method. Their anti-oxidant, anti-wrinkle anti-melanogenic tested vitro using human keratinocyte HaCaT exposed to UVB radiation melanocyte B16F10 cells. Multi-omics data analysis identified key bioactive molecules collagen formation, pigmentation, oxidative stress inflammation. In screenings effects, compound-loaded outperformed sEV- compound-only treatments. Specifically UVB-exposed cells, 2 μg/mL sEVs 20 NR, RES, 5 VITC reduced reactive oxygen species level 22.0%; while combination 2.8 RET significantly MMP3 upregulated PLOD1 expressions. incubated 0.5 mM ARB had intracellular melanin content lowered 21.4% 22.4% respectively. All caused no cytotoxicity. Our study demonstrated superiority deliver both endogenous biocargos exogenous achieve effects.

Language: Английский

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Effectiveness of Extracellular Vesicle Application in Skin Aging Treatment and Regeneration: Do We Have Enough Evidence from Clinical Trials?

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2354 - 2354

Published: March 6, 2025

In recent years, there has been a dynamic development in therapies utilizing extracellular vesicles (EVs) including exosomes. Therefore, we have conducted an analysis of the scientific literature to verify current state knowledge about these therapies. A total 12 clinical studies were analyzed, covering use EVs treating skin aging, acne scars, alopecia, and wound healing. The results indicate that exosomes hold potential regenerative therapies, offering innovative non-invasive therapeutic approaches. At same time, significant challenges related standardization their production lack large-scale randomized identified. Thus, also evaluated investigated trials regard MISEV (Minimal Information for Studies Extracellular Vesicles) criteria. This review provides comprehensive overview contemporary applications therapy medicine, highlighting directions further research.

Language: Английский

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Human Skin Microbiota‐Derived Extracellular Vesicles and Their Cosmeceutical Possibilities—A Mini Review

Experimental Dermatology, Journal Year: 2025, Volume and Issue: 34(3)

Published: March 1, 2025

The human skin hosts a complex ecosystem of microorganisms, collectively termed the microbiota. This intricate microbial community plays pivotal role in health and disease. Microbes interact with host cells immune through products such as metabolites secreted proteins. Research recent years has received significant attention towards extracellular vesicles (EVs)-mediated microbe-host communication. In this concise review, we discuss microbiota EVs regulation maintenance functional dermal tissue. topical is predominantly composed bacteria, Actinobacteria, Firmicutes, Proteobacteria Bacteroidetes being predominant phyla. Fungi, particularly Malassezia species, also constitute component ecosystem. Nevertheless, research on primarily focused limited number bacterial fungal species pertaining to skin, including Staphylococcus aureus, epidermidis, Propionibacterium acnes, Lactobacillus plantarum sympodialis. Particularly, derived from epidermidis show promising outcome management inflammation ageing. Given demonstrated ability penetrate deliver beneficial compounds, their application cosmetic cosmeceutical remains its early stages. Accordingly, address need for extensive research, challenges opportunities fully harness potential skincare regimens.

Language: Английский

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The role of exosomal lncRNAs in mediating apoptosis and inflammation in UV-induced skin photoaging

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: April 14, 2025

The skin, as the body’s largest organ, functions a vital barrier against environmental insults. Chronic exposure to ultraviolet (UV) radiation significantly contributes premature aging, or photoaging, which leads DNA damage and disrupts repair mechanisms. Exosomes, are small extracellular vesicles, play key role in cell-to-cell communication might help mitigate effects of photoaging by transporting bioactive molecules skin cells. Long non-coding RNAs (lncRNAs) increasingly recognized for their regulatory roles process, influencing stress responses repair; however, involvement exosomes context aging is not yet well understood. In this study, we developed model using SD rats subjected UVA UVB irradiation, led significant changes dermis such increased dryness, wrinkles, pigmentation, vascular alterations. Histological evaluations showed uneven thickening epidermis, degradation collagen elastic fibers, cellular infiltration. Exosomes isolated from dermal tissues exposed UV displayed altered size distributions. Transcriptomic analyses UV-treated identified 2,332 lncRNAs 5,906 mRNAs that were differentially expressed, revealing pathways related oxidative stress, apoptosis, responses. A cis-regulatory analysis 1,327 essential interactions between mRNAs, highlighting controlling inflammation apoptosis. Importantly, both IL-1B GADD45B levels UV-challenged HaCaT cells, indicating crucial responding UV-induced stress. This study highlights exosomal managing reactions impacting associated with inflammation, These insights pave way development lncRNA-focused therapeutic approaches address damage.

Language: Английский

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Iris germanica L. Rhizome-Derived Exosomes Ameliorated Dihydrotestosterone-Damaged Human Follicle Dermal Papilla Cells Through the Activation of Wnt/β-Catenin Pathway

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4070 - 4070

Published: April 25, 2025

Hair loss is often associated with oxidative stress and mitochondrial dysfunction in human follicle dermal papilla cells (HFDPCs), resulting impaired cellular function degeneration. Thus, many studies have been conducted on natural plants aimed at inhibiting hair loss. This study investigated the therapeutic potential of exosomes derived from rhizomes Iris germanica L. (Iris-exosomes) HFDPCs damaged by dihydrotestosterone (DHT). Iris-exosomes significantly reduced reactive oxygen species (ROS) levels, restoring membrane ATP production, thereby mitigating improving function. These effects occurred alongside enhanced processes critical for regeneration, including increased cell migration, alkaline phosphatase (ALP) activity, three-dimensional (3D) spheroid formation, which replicates follicle-like microenvironment promotes inductive potential. Furthermore, stimulated Wnt/β-catenin signaling pathway enhancing glycogen synthase kinase-3β (GSK-3β), AKT, extracellular signal-regulated kinase (ERK), leading to β-catenin stabilization nuclear translocation, supporting expression genes essential growth. Taken together, these findings suggest that can be promising ingredients alleviating

Language: Английский

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Effectiveness of a Novel Compound HAIR & SCALP COMPLEX on Hair Follicle Regeneration

Cosmetics, Journal Year: 2024, Volume and Issue: 11(1), P. 10 - 10

Published: Jan. 16, 2024

Background: People lose between 50 and 100 hairs a day generate new ones from stem cells in hair follicles, but those suffering baldness, the remain inactive are unable to regenerate hair. Although 9% of follicles telogen at any time, variety factors, including growth factors cytokines, promote transition anagen subsequent stimulation growth. Methods: We compared vitro, on cultures human effect regeneration dermal papilla plant-derived nanovesicles, exosomes cord blood bovine colostrum, mixture cytokines purified called GF20, compound HAIR & SCALP COMPLEX obtained by adding isolated colostrum GF20. Results: The analyses demonstrated significant increase bulb samples treated with other elements tested. Conclusions: In this research, we propose possible treatment that could help significantly slow down loss encourage growth: COMPLEX.

Language: Английский

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Medicalized Aesthetic Uses of Exosomes and Cell Culture-Conditioned Media: Opening an Advanced Care Era for Biologically Inspired Cutaneous Prejuvenation and Rejuvenation

Cosmetics, Journal Year: 2024, Volume and Issue: 11(5), P. 154 - 154

Published: Sept. 7, 2024

Recent advancements in aesthetic medicine offer innovative cosmetic solutions to enhance patient skin quality and appearance. Advanced treatment options enable practitioners effectively address aging signs, pigmentation imbalance, loss of elasticity ambulatory home-based care regimens. Exosomes (nanoscale cell-derived vesicles) transport a variety biomolecules are pivotal physiological intercellular communication. Importantly, exosomes have recently emerged as key endogenous players tissular regeneration. More broadly (from an active ingredient purity standpoint), exosomes, stem cell secretomes, culture-conditioned media been clinically proven exert multifaceted beneficial topical effects (anti-inflammatory, antioxidant, anti-aging, rejuvenation). Therefore, human, animal, plant-derived or other refined sub-cellular biological fractions gaining substantial interest within the industries. Notably, such approaches thought be among most promising novel contenders for advanced, biologically inspired prejuvenation rejuvenation care. The present narrative review summarizes latest oriented research on media, highlighting their mechanisms action various applications. Furthermore, it explores innovation landscape currently commercially available products global market discusses potential future applications ingredients medical industry.

Language: Английский

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Cell membrane and extracellular vesicle membrane‐coated nanoparticles: An envisaged approach for the management of skin conditions

View, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Abstract Cell membrane‐coated biomimetic nanosystems have been recognized as promising drug delivery vehicles in recent years for the management of diverse skin conditions. Nanoparticles (NPs) coated with biomembranes, derived from either cell membranes or subcellular structures (e.g., extracellular vesicles), offer an opportunity to combine biological interfacial characteristics coating alongside internal core component at nanoscale. The enhances biocompatibility NPs and their interaction skin, improving affinity, contact, retention. This also enables controlled release drugs provides skin‐targeting capabilities, which collectively improve effectiveness, safety, stability topical transdermal formulations. In this context, current review delves into progress using therapeutics. Specifically, it examines various types coatings, including origins, heterogeneous functions, surface molecular repertoires, great detail. Additionally, presents methods preparing characterizing biomimetic‐coated NPs. Furthermore, potential bioinspired treating a range skin‐related conditions has meticulously explored. Last, toxicological aspects these thoroughly examined provide thorough summary evolution applications.

Language: Английский

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Skin necrosis after intradermal injection of lyophilized exosome: A case report and a review of the literature

Journal of Cosmetic Dermatology, Journal Year: 2024, Volume and Issue: 23(5), P. 1597 - 1603

Published: Feb. 7, 2024

Abstract Background Exosomes have gained attention for their potential in skin rejuvenation. Currently, most exosome products are available topical administration, and the use of subdermal injection as a route administration has not been approved. Aims The purpose this case report is to describe necrosis that occurred following an intradermal lyophilized exosomes. Materials Methods We hereby middle‐aged man who experienced adverse effects after receiving Multiple injections product were administered treat enlarged facial pores. Shortly injection, patient felt pain noticed several dark red bumps. Three days lesions transformed into palpable, painful, non‐blanchable purplish papules nodules, accompanied by central, tiny crusted erosions. residual was injected upper arm using method. Similar also appeared, biopsy showed necrotic keratinocytes, leukocytoclastic vasculitis, eccrine necrosis. Results There few reports regarding complications, especially those related These complications include multiple foreign‐body granulomatous reactions at sites. In our case, oral prednisolone duration 7 days. After treatment, exhibited notable improvement, eventually leaving post‐inflammatory hyperpigmentation. Conclusion Utilizing exosomes through unapproved methods should be avoided due possibility could cause aesthetic issues.

Language: Английский

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