Identification and characterization of silver nanoparticles from Erythrina indica and its antioxidant and Uropathogenic antimicrobial properties

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 190, P. 106635 - 106635

Published: April 4, 2024

Language: Английский

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Synthesis of L-Lactide from Lactic Acid and Production of PLA Pellets: Full-Cycle Laboratory-Scale Technology

Polymers, Journal Year: 2024, Volume and Issue: 16(5), P. 624 - 624

Published: Feb. 25, 2024

Lactide is one of the most popular and promising monomers for synthesis biocompatible biodegradable polylactide its copolymers. The goal this work was to carry out a full cycle production from lactic acid. Process conditions ratios reagents were optimized, key properties synthesized polymers investigated. influence molecular weight acid oligomers on yield lactide studied. polymerization first carried in 500 mL flask then scaled up 2000 laboratory reactor setup with combined extruder. Initially, solution concentrated remove free water; then, oligomerization presence various concentrations tin octoate catalyst at temperatures 150 210 °C. 67–69%. resulting raw purified by recrystallization solvents. after butyl acetate (selected as optimal solvent purification) 41.4%. Further, unit concentration ppm. Conversion 95%; Mw = 228 kDa; PDI 1.94. products studied differential scanning calorimetry, NMR spectroscopy gel permeation chromatography. form pellets obtained using an extruder pelletizer.

Language: Английский

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The role of multi-walled carbon nanotubes in enhancing the hydrolysis and thermal stability of PLA

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 10, 2024

Polylactic acid (PLA) is a bioresorbable and biodegradable polymer extensively used in various biomedical engineering applications. In this study, we investigated the mass loss thermal properties of PLA-multi-walled carbon nanotube (MWCNT) composites under simulated physiological conditions. The were prepared by melting PLA with 0.1, 0.5, 1.0, 5.0 wt% MWCNTs using an ultrasonic agitator, FTIR analysis confirmed composite formation. Subsequently, subjected to hydrolysis conditions (pH 7.4 37 °C) for up 60 days. results revealed that decreased increasing MWCNT content, suggesting presence decelerated process. On day 58, pure was 12.5%, decreasing 8.34% 0.1% MWCNT, 5.94% 0.5% 4.59% 1% 3.54% 5.0% MWCNT. This study offers valuable insights into behavior PLA-MWCNT physiologically conditions, facilitating development new enhanced stability degradation resistance

Language: Английский

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A morphological analysis of calcium hydroxylapatite and poly‐l‐lactic acid biostimulator particles

Skin Research and Technology, Journal Year: 2024, Volume and Issue: 30(6)

Published: June 1, 2024

Injectable fillers, pivotal in aesthetic medicine, have evolved significantly with recent trends favoring biostimulators like calcium hydroxylapatite (CaHA-CMC; Radiesse, Merz Aesthetics, Raleigh, NC) and poly-l-lactic acid (PLLA; Sculptra Galderma, Dallas, TX). This study aims to compare the particle morphology of these two injectables examine its potential clinical implications. Utilizing advanced light scanning electron microscopy techniques, physical characteristics CaHA-CMC PLLA particles were analyzed, including shape, size, circularity, roundness, aspect ratio, quantity phagocytosable particles. The findings reveal several morphological contrasts: exhibited a smooth, homogenous, spherical diameters predominantly ranging between 20 45 µm, while varied considerably shape appearing as micro flakes from 2 150 µm major axis length. circularity roundness higher compared PLLA, indicating more uniform shape. Aspect ratio analysis further underscored differences, showing closer resemblance circles, unlike oblong Quantification content both revealed percentage PLLA. These distinctions may influence tissue response each treatment. CaHA-CMC's uniform, result reduced inflammatory cell recruitment, whereas PLLA's heterogeneous evoke pronounced response.

Language: Английский

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Consensus Recommendations for the Reconstitution and Aesthetic Use of Poly-D,L-Lactic Acid Microspheres

Clinical Cosmetic and Investigational Dermatology, Journal Year: 2024, Volume and Issue: Volume 17, P. 2755 - 2765

Published: Dec. 1, 2024

Abstract: Poly-D,L-lactic acid (PDLLA) microspheres, marketed globally as Aesthefill ® (Regen Biotech, Seoul, South Korea), are recognized for their biocompatible and biostimulatory properties, positioning them a preferred option in aesthetic medicine. This article presents consensus recommendations from Brazilian experts on the reconstitution clinical application of PDLLA facial non-facial treatments. Developed using modified Delphi method with contributions leading dermatologists plastic surgeons, outlines protocols reconstitution, injection techniques, patient management. Key include 7– 8 mL sterile water injection, addition lidocaine to improve comfort, preference targeting superficial subcutaneous layer. Dosing guidelines specifically tailored each treatment area desired degree correction, underscoring importance personalized plans. Maintenance treatments advised at biennial intervals or shorter patients exhibiting accelerated collagen degradation. The also highlights need proper training screening minimize adverse effects, such nodules granulomas. comprehensive guide aims standardize use PDLLA, prioritizing safety optimizing outcomes. While trials evaluating PDLLA's indications remain limited, these evidence-based bridge gap by offering practical grounded expertise. Further research is encouraged validate explore new applications Plain Language Summary: Poly-D, L-lactic microspheres small particles used medicine skin appearance stimulating production. summary expert preparing body Why was study done? aimed create standardized help practitioners achieve best results ensure when cosmetic purposes. What did researchers do find? They called approach gather refine PDLLA. By consensus, who participated this recommend prepare adding reduce discomfort during injections. Specific linear retrograde fanning methods, different areas face body. have provided dosing emphasize individualized mean? more effectively safely, providing better outcomes patients. Regular maintenance suggested every two years, frequent sessions those faster loss. Proper careful essential avoid complications Keywords: poly-D,L-lactic acid, polylactic stimulator, recommendations, filler

Language: Английский

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Comparative analysis of collagen-stimulating effects of poly-L-lactic acid, poly-D, L-lactic acid, and polycaprolactone subcutaneous injections in porcine model

Dermatologica Sinica, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Dear Editor, Biostimulators are cutting-edge cosmetic treatments that rejuvenate the skin by stimulating collagen production. Poly-L-lactic acid (PLLA; Sculptra; Galderma), poly-D, L-lactic (PDLLA; AestheFill, REGEN), and polycaprolactone (PCL; Ellansé; Sinclair Pharma) stimulate biosynthesis of type I (COL1), thereby remodeling dermal architecture, augmenting thickness elasticity, achieving volumization wrinkle correction.[1] have been shown to induce production III (COL3), which functions as a subcutaneous scaffold plays crucial role in reducing superficial facial static wrinkles.[2] This study investigated ability these three biostimulators synthesis at different time points. PLLA, PDLLA, PCL were subcutaneously injected into dorsal six adult Lee-Sung minipigs [Table S1]. Skin samples obtained 28, 91, 182 days after injection, was examined. The procedures detailed Supplementary Materials. After harvested, quantitative reverse transcription polymerase chain reaction performed determine mRNA expression levels COL1 COL3. In addition, immunohistochemistry staining on assess COL3 densities. At 28 postinjection, PLLA led higher compared PDLLA. Furthermore, 91 182, increase induced remained significantly greater than observed with other two biostimulators. Similarly, days, resulted PDLLA [Figure 1]. However, no significant differences noted among Histological analysis confirmed stimulated density fibers 2].Figure 1: Quantitative following stimulation days. Note: *P < 0.05, **P 0.01. NS: significance.Figure 2: Density (depicted brown) assessed using immunohistochemical staining. Noticeable variations day 28. Representative histological micrographs from animal specimens Y171 (day 28), Y173 91), Y167 182) presented. Scale bar = 50 μm.The factors influencing degree between material host include composition, size, shape, surface roughness, porosity biostimulator. this study, increased injection may be attributed several factors. First, has slower degradation rate,[3,4] leading its prolonged presence within deep dermis or layer sustained activation fibroblasts. Second, induces more pronounced foreign body due rough irregular fibroblasts.[5] These effects less their faster prominent reactions induced. Differences chemical structure also influence Further research is needed fully understand mechanisms evaluate long-term 3 limitations. functionality (i.e., tensile strength elasticity contribution) newly produced not evaluated, limiting our understanding practical 6-month observation period capture quality. Longer-term studies essential durability sustainability effects. porcine model revealed superior production, effect for least while initially diminished indicating impact primarily driven rather Although elevated can contribute structural support, excessive pose risk scarring fibrosis. Therefore, remains promising candidate enhancing rejuvenation, it carefully optimize dosing application techniques minimize potential adverse outcomes. Future should focus validating findings human subjects evaluating safety efficacy various clinical settings, well exploring use combination therapies maximize both aesthetic regenerative medicine. Ethical approval Institutional Animal Care Use Committee (IACUC) stutas: NLAC-111-M-024. Data availability statement datasets generated during and/or analyzed current publicly available, but available corresponding author reasonable request. Financial support sponsorship Nil. Conflicts interest Dr. Jeff Huang Cheng-Chieh, Jason Li Chien-Nien, Luiz Eduardo Toledo Avelar, Alessandra Haddad Stephanie Lam speakers medical consultant Galderma. Leon Tsung-Ju Lee conflict disclose.

Language: Английский

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Development and Characterization of PEGylated Poly D,L-Lactic Acid Nanoparticles for Skin Rejuvenation

Nanomaterials, Journal Year: 2025, Volume and Issue: 15(6), P. 470 - 470

Published: March 20, 2025

Recently, various biocompatible and biodegradable materials have garnered significant attention as cosmetic fillers for skin rejuvenation. Among these, poly ε-caprolactone (PCL), L-lactic acid (PLLA), D,L-lactic (PDLLA), polydioxanone (PDO) microspheres been developed commercialized a dermal filler. However, its irregularly hydrophobic pose hydration challenges, often causing syringe needle blockages side effects such delayed onset nodules papules after the procedure. In this study, we synthesized polyethylene glycol-poly (mPEG-PDLLA) copolymer to address limitations of conventional polymer fillers. Comprehensive characterization was performed using nuclear magnetic resonance spectroscopy, Fourier transform infrared differential scanning calorimetry. The mPEG-PDLLA copolymers demonstrated unimodal size distribution approximately 121 ± 20 nm in an aqueous solution. vitro cytotoxicity collagen genesis were evaluated human fibroblast cells. angiogenesis observed over time hairless mice injected with copolymers, confirming potential role enhancing synthesis. To assess inflammatory response, expression levels genes MMP1 IL-1β analyzed. Additionally, gene transforming growth factor-β types I III compared Rejuran® animal studies. newly collagen-stimulating PEGylated PDLLA may be safe effective option

Language: Английский

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Ablative Fractional Lasers Versus Needle-Based Devices for Poly-l-Lactic Acid Delivery: An Optical Coherence Tomography and Histology Study

Dermatologic Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Poly-l-lactic acid (PLLA) is widely used in dermatology, traditionally through subcutaneous injections. Alternative delivery methods such as ablative fractional lasers (AFLs) and needle-based devices have gained interest, but limited data exist on their efficacy. To assess the effectiveness of AFLs for PLLA impact channel morphology uptake. Full-thickness human abdominal skin samples were treated with CO2 laser, difference frequency generation (DFG) microneedle radiofrequency (MNRF), (MN). After applying topically, real-time particle movement into channels was detected optical coherence tomography (OCT). Histology assessed vertical distribution PLLA. Optical captured particles descending from AFLs, not MNRF or MN. confirmed uptake up to 80.3% 61.9% DFG channels, compared minimal (1.4%) MN (0.7%). The laser enabled deeper penetration (120-240 μm), whereas delivered more superficially (0-120 μm). Ablative effectively skin, while showed Among geometry influenced distribution. In vivo clinical studies are needed retention its remodeling.

Language: Английский

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A high clinically translatable strategy to anti-aging using hyaluronic acid and silk fibroin co-crosslinked hydrogels as dermal regenerative fillers

Acta Pharmaceutica Sinica B, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Efficacy and Safety of Poly-l-Lactic Acid in Facial Aesthetics: A Systematic Review

Polymers, Journal Year: 2024, Volume and Issue: 16(18), P. 2564 - 2564

Published: Sept. 11, 2024

The primary objective of this systematic review study was to investigate the effectiveness, durability, and adverse events PLLA treatment for aesthetic indications. search strategy performed in MEDLINE (Ovid). electronic literature five databases performed, from inception until 12th February 2024. This identify randomized clinical trials that assessed adult individuals exhibiting facial aging and/or lipoatrophy. Risk bias using Cochrane Risk-of-Bias Tool Randomized Trials (RoB 2). Eleven RCTs out 1467 identified citations were included. Four studies showed increased dermal thickness, significant improvement lipoatrophy severity scores, after with its effects sustained at least 25 months. Two demonstrated superiority over injectable human collagen. Also, three positive results favoring when compared PH gel severity, transepidermal water loss, skin quality, elasticity, patient satisfaction. All mild-to-moderate intensity, main ones worth noting bruising, hematoma, tenderness, nodules, edema. Five eleven considered having high risk bias. evidence on effectiveness safety rejuvenation is low quality; thus, reported safety, long-lasting purpose should be further investigated.

Language: Английский

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