Efficacy of Erwinia amylovora and Xanthomonas campestris pv campestris phages to control fire blight and black rot in vivo DOI Creative Commons
Gloria Vique, Elena Mendoza-Barberà, María Dolores Ramos‐Barbero

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: May 16, 2025

ABSTRACT Phytopathogens, such as Erwinia amylovora and Xanthomonas campestris , pose significant threats to agriculture, leading substantial economic losses. Traditional chemical pesticides can harm soil fertility, contaminate water, impact non-target organisms natural predators pollinators, highlighting the need for sustainable pest control methods. This study explores use of bacteriophages biocontrol agents against E. which causes fire blight, X. pv. responsible black rot in cruciferous vegetables. Bacteriophages were isolated from urban wastewater tested their lytic activity these pathogens. Three virulent phages identified: ɸEF1 ɸEF2 ɸXF1 . Genetic analysis confirmed absence known lysogeny-related genes, indicating that are ideal candidates applications. In vitro assays demonstrated bacterial population reductions. Specifically, killed 92.1% at a multiplicity infection (MOI) 1 after 3 h, while reduced by 98.1%. When combined 1:1 ratio, two populations 99.7%, no regrowth resistant cells was observed, not case when applied individually. 99.9% an MOI 5 h. vivo experiments using pears kohlrabi models further validated phage effectiveness. Treated showed blight symptoms, plants exhibited markedly less necrosis compared untreated controls. IMPORTANCE new have been isolated: targeting one All able rapidly reduce corresponding phytopathogens alleviate disease symptoms plant models. These findings highlight potential managing diseases, offering alternative traditional treatments.

Language: Английский

Effects of Grazing Intensity on Microbial Diversity at Different Soil Depths in Desert Steppe Soils DOI Creative Commons

Yuxin Wang,

Xin Ju, Qian Wu

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(1), P. 124 - 124

Published: Jan. 6, 2025

This study examines the influence of grazing intensity on soil microbial communities in a desert steppe ecosystem. Soil samples were collected from three depths (0–10 cm, 10–20 and 20–30 cm) under varying intensities: control (CK), light (LG), moderate (MG), heavy (HG). Key physicochemical properties plant characteristics analyzed alongside diversity community composition, which assessed by identifying amplicon sequence variants conducting linear discriminant analysis effect size. The results showed that significantly impacted moisture, organic carbon, total nitrogen, phosphorus levels, with notable decrease cover grazing. CK LG treatments supported higher diversity, especially surface layers, while was associated shift composition toward stress-tolerant taxa like Acidobacteriota Blastocatella. Non-metric multidimensional scaling revealed differences structure between depths, effects diminishing depth. These findings highlight critical role sustainable practices maintaining health implications for long-term resilience ecosystems.

Language: Английский

Citations

1
Influence of Cover Crop Root Functional Traits on Sweet Potato Yield and Soil Microbial Communities DOI Creative Commons
Xinyi Chen, Jie Zhang, Wei Xia

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(3), P. 471 - 471

Published: Feb. 20, 2025

The symbiotic relationship between cover crops and soil microorganisms is closely linked to nutrient cycling crop growth within agroecosystems. However, how with different root functional traits influence microbial communities, properties, yields has remained understudied. This study assessed the of hairy vetch (HV) rapeseed (RP), along sweet potato yield, enzyme activity under red dryland conditions. High-throughput sequencing was also employed characterize diversity, composition, network structure bacterial fungal communities. According plant economic spectrum theory our research results on traits, HV can be identified as a resource-acquisitive crop, RP treatment resource-conservative crop. Although did not significantly increase rate reached 8.49%. Resource-conservative were associated increased pH, SOC, TP, which enhanced species diversity boosted populations Chloroflexi Alphaproteobacteria. In contrast, promoted proliferation Gammaproteobacteria. Network analysis indicated that facilitated complexity through intensified intra-community competition. Resource-acquisitive stability Collectively, these findings underscore distinct advantages varying in shaping Appropriate rotations effectively regulate communities hold potential enhance yield.

Language: Английский

Citations

0
Assessment of some biological properties of soils across selected farming communities of Southern and Northern Nigeria DOI Creative Commons

Matthew Banji Oyun,

Adebayo Jonathan Adeyemo,

Taiwo Ejiola Mafimisebi

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Abstract Background and Aims: This study examines the impact of soil biological parameters on agricultural productivity sustainability across Nigeria's diverse climates. Materials Methods: A multistage sampling method was adopted to collect 108 samples from four southern northern Nigeria locations, three communities, farmers' fields replicated times in a Complete Randomized Block Design (RCBD). Each sample analyzed for initial physicochemical some selected microbial properties. Results: In humid regions, activity high Location 1 (L1), with bacterial densities reaching 12.31 x 107 CFU/ml associated fungal yeast at 1.55 106 SFU/ml 5.08 counts, respectively. indicates bacterial-dominated ecosystem favourable rapid nutrient cycling, although it may risk structure over long term if unmanaged. Conversely, 3 (L3) drier areas showed less 9.83 but higher populations. suggests more environment that could enhance cycling structure, which is essential arid regions. The also investigated how environmental factors farming practices influence balances through hierarchical clustering analysis. Clusters like L3C1F2 L3C2F3 demonstrated balanced ecosystems substantial populations, supporting robust health. Conclusion: research highlights need precise, region-specific accommodate local profiles optimize crop yields sustain health, enhancing food security economic stability Nigeria.

Language: Английский

Citations

0
The Role of Rhizosphere Microbiomes in Enhancing Terpene Production in Ferula asafoetida: A Comparative Study Across Three Different Regions DOI

Masoud Ghayyoumi,

Abbasali Emamjomeh, Seyed Javad Davarpanah

et al.

Rhizosphere, Journal Year: 2025, Volume and Issue: unknown, P. 101054 - 101054

Published: March 1, 2025

Language: Английский

Citations

0
Utilizing Microbial Inoculants to Alleviate Continuous Cropping Obstacles: Insights into the Metabolites and Transcriptomic Responses of Pinellia ternata DOI Creative Commons
Xinyu Wang, Mohammad Murtaza Alami, Shiyuan Gong

et al.

Metabolites, Journal Year: 2025, Volume and Issue: 15(3), P. 189 - 189

Published: March 11, 2025

Pinellia ternata (Thunb.) Breit is a widely used medicinal herb in Traditional Chinese Medicine (TCM). Still, its sustainable cultivation threatened by continuous cropping obstacles, which disrupt soil ecosystems, reduce yield, and degrade quality. Objectives: This study explores the potential of microbial inoculants to mitigate these challenges through integrated metabolomic transcriptomic analyses. Methods: Soil samples from fields with without issues were compare effects on secondary metabolism gene expression P. ternata. Results Discussion: Metabolomic profiling identified 20,969 metabolites, significant changes lipid-like molecules (22.2%), organic acids (9.1%), phenylpropanoids (7.0%) under treatment. Notable increases phenylalanine caffeic acid levels observed microbial-inoculated plants. Correspondingly, analysis revealed upregulation ammonia-lyase (PAL) other stress-related genes, confirming metabolic shifts. Clustering machine learning analyses highlighted critical roles metabolites genes enhancing plant resilience. Microbial improved metabolite production. Implications: These findings provide valuable insights into mechanisms microbial-plant interactions establish approach for cultivating ternata, addressing while improving crop productivity

Language: Английский

Citations

0
Synergistic effect of Zinc Solubilizing Bacteria and Consortia on the zinc marker enzymes and gaseous exchange parameters in Rice (Oryza sativa L.) for zinc biofortification DOI
Tribhuwan Singh,

Munmun Kothari,

Sudershan Mishra

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109807 - 109807

Published: March 1, 2025

Language: Английский

Citations

0
Livestock Slurry and Sustainable Pasture Management: Microbial Roles, Environmental Impacts, and Regulatory Perspectives in Ireland and Europe DOI Creative Commons

Messias José Dos Santos SILVA,

Ismin Zainol Abidin, João Rui Tanoeiro

et al.

Microorganisms, Journal Year: 2025, Volume and Issue: 13(4), P. 788 - 788

Published: March 29, 2025

Pastures serve as the primary source of grass and forage plants for grazing livestock, requiring adequate nutrient input to sustain growth soil fertility. Slurry from livestock industry is widely utilized a sustainable cost-effective alternative chemical fertilizers. Microorganisms within slurry–pasture system are essential breaking down organic matter, facilitating cycling, improving health. However, mismanagement or inefficient microbial decomposition can lead significant issues, such leaching into water bodies, causing eutrophication, antimicrobial resistance, reduced availability in pastures, which, turn, may negatively impact productivity. Thus, this paper investigates composition benefits slurry pasture management, highlights roles evaluates regulatory frameworks Ireland Europe. Additionally, it examines environmental risks associated with improper application, providing insights support management practices.

Language: Английский

Citations

0
Dual film-controlled model urea improves summer maize yields, N fertilizer use efficiency and reduces greenhouse gas emissions DOI
Yifeng Li, Wenfei Yang, Wanxin Wang

et al.

Soil and Tillage Research, Journal Year: 2025, Volume and Issue: 252, P. 106565 - 106565

Published: April 8, 2025

Language: Английский

Citations

0
Resilience and Adaptation: Plant Ecology in Indonesia’s Geothermal Environments DOI
Ghazi Mauer Idroes, Khairan Khairan, Eko Suhartono

et al.

Leuser Journal of Environmental Studies, Journal Year: 2025, Volume and Issue: 3(1), P. 44 - 55

Published: April 26, 2025

Geothermal ecosystems are defined by extreme environmental conditions, such as elevated temperatures, high concentrations of toxic chemicals, and fluctuations in abiotic stressors, which shape plant survival adaptation. These unique ecosystems, found across various geothermal regions globally, support specialized communities that have developed distinctive morphological, physiological, ecological adaptations. Indonesia, located on the Pacific Ring Fire, is one world’s richest nations, offering an important yet underexplored context for studying vegetation zones. This review examines conditions adaptive strategies vegetation, patterns diversity within Indonesian fields. It also explores succession, community dynamics, potential use indicators biomonitoring. Despite growing interest, significant research gaps remain, particularly long-term monitoring integration molecular-level studies. Addressing these essential enhancing scientific understanding informing conservation sustainable energy development tropical regions. highlights significance underscores need interdisciplinary to both biodiversity preservation responsible exploitation.

Language: Английский

Citations

0
The Impact of Flooding on Soil Microbial Communities and Their Functions: A Review DOI Creative Commons
Ashim Kumar Das,

Da‐Sol Lee,

Youn-Ji Woo

et al.

Stresses, Journal Year: 2025, Volume and Issue: 5(2), P. 30 - 30

Published: May 2, 2025

Soil microorganisms provide multifaceted benefits, including maintaining soil nutrient dynamics, improving structure, and instituting decomposition, all of which are important to health. Unpredictable weather events, flooding from heavy rainfall, flash floods, seawater intrusion, profoundly impact ecology, is primarily challenged by stress, imbalances these microbial communities their functions. This disturbance impairs the symbiotic exchanges between microbes plants limiting root exudates habitats for microbes, as well acquisition efficiency plants. Therefore, this review comprehensively examines changes in that occur under conditions. Flooding reduces oxygen (O2) levels, aerobic but promoting anaerobic ones, potential pathogens. In flooded soil, O2 deficiency indirectly depends on size particles water turbidity during flooding. depletion critical shaping community adaptation, linked variations pH, concentrations, redox status, fresh saline vary differently terms adaptation microorganisms. Wet alters enzyme activity, influences composition. Notably, three-month post-flooding conditions allow adapt stabilize more effectively than once-weekly frequency. Based presence aboveground species, fungi found reduce conditions, while nematode numbers, surprisingly, increase. Direct indirect impacts physio-chemical properties indicate positive or negative feedback loops influence ecosystem. Over years, beneficial such plant-growth-promoting (PGPMs) have been identified regulating nutrients wetland environments, thereby enhancing health better plant growth development. Overall, understanding mechanisms belowground ecosystems essential optimizing agricultural practices ensuring sustainable crop production flood-prone areas.

Language: Английский

Citations

0