Comparative Evaluation of Rapid Isothermal Amplification and Antigen Assays for Screening Testing of SARS-CoV-2 DOI Creative Commons
Nol Salcedo, Brena F. Sena,

Xiying Qu

et al.

Viruses, Journal Year: 2022, Volume and Issue: 14(3), P. 468 - 468

Published: Feb. 25, 2022

Human transmission of SARS-CoV-2 and emergent variants concern continue to occur globally, despite mass vaccination campaigns. Public health strategies reduce virus spread should therefore rely, in part, on frequent screening with rapid, inexpensive, sensitive tests. We evaluated two digitally integrated rapid tests assessed their performance using stored nasal swab specimens collected from individuals or without COVID-19. An isothermal amplification assay combined a lateral flow test had limit detection 10 RNA copies per reaction, positive percent agreement (PPA)/negative (NPA) during the asymptomatic symptomatic phases 100%/100% 95.83/100%, respectively. Comparatively, an antigen-based 30,000 PPA/NPA 82.86%/98.68% 91.67/100%, Both optimized peak period transmission; however, reduced sensitivity clinical samples qPCR Ct values greater than 29.8. Low-cost, high-throughput enabled by techniques have value for outbreak control.

Language: Английский

Fatal Myocarditis following COVID-19 mRNA Immunization: A Case Report and Differential Diagnosis Review DOI Creative Commons
Pedro Manuel Barros de Sousa,

Elon Almeida Silva,

Marcos Adriano Garcia Campos

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(2), P. 194 - 194

Published: Feb. 13, 2024

Carditis in childhood is a rare disease with several etiologies. We report case of infant death due to pericarditis and myocarditis after the mRNA vaccine against COVID-19 (COVIDmRNAV). A 7-year-old male child received first dose COVIDmRNAV presented monoarthritis fever non-responsive oral antibiotics. The laboratory investigation showed signs infection (leukocytosis, high levels c-reactive protein). His condition rapidly deteriorated, patient died. autopsy identified pericardial fibrin deposits, hemorrhagic areas myocardium, normal valves. diffuse intermyocardial inflammatory infiltrate composed T CD8+ lymphocytes histiocytes was identified. An antistreptolysin O (ASO) dosage titers. presence arthritis, elevated ASO, carditis fulfills criteria for rheumatic fever. However, valve Aschoff’s nodules, present 90% cases, were absent this case. temporal correlation vaccination prompted its inclusion as one In cases myocardial damage related COVID-19mRNAV, it appears be expression exosomes lipid nanoparticles, leading cytokine storm. potential effects COVID-19mRNAV must considered pathogenesis disease, whether an etiology or contributing factor previously initiated injury.

Language: Английский

Citations

3
Pediatric Multisystem Syndrome Associated with SARS-CoV-2 (MIS-C): The Interplay of Oxidative Stress and Inflammation DOI Open Access
Serafina Perrone, Laura Cannavò, Sara Manti

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 12836 - 12836

Published: Oct. 25, 2022

Pediatric inflammatory multisystem syndrome temporally associated with SARS-CoV-2 (MIS-C) is characterized by persistent fever and evidence of single or multiorgan dysfunction, laboratory inflammation, elevated neutrophils, reduced lymphocytes, low albumin. The pathophysiological mechanisms MIS-C are still unknown. Proinflammatory mediators, including reactive oxygen species decreased antioxidant enzymes, seems to play a central role. Virus entry activates NOXs inhibits Nrf-2 response inducing free radicals. biological functions nonphagocytic under study appear include: defense epithelia, intracellular signaling for growth regulation cell differentiation, post-translational modifications proteins. This educational review has the aim analyzing newest on role oxidative stress (OS) in MIS-C. Only relating mediators OS evaluation children following infection will it be possible achieve better understanding these reduce long-term morbidity. link between inflammation key developing effective prevention strategies antioxidants protect children.

Language: Английский

Citations

14
Acute Myocardial Infarction in COVID-19 Patients—A Review of Literature Data and Two-Case Report Series DOI Open Access

Luiza Nechita,

Elena Niculeț, Liliana Baroiu

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(10), P. 2936 - 2936

Published: May 16, 2024

Background/Objectives: The newly emergent COVID-19 pandemic involved primarily the respiratory system and had also major cardiovascular (CVS) implications, revealed by acute myocardial infarction (AMI), arrhythmias, injury, thromboembolism. CVS involvement is done through main mechanisms-direct indirect heart muscle with high mortality rates, worse short-term outcomes, severe complications. AMI echo of injury (revealed increases in CK, CK-MB, troponin serum markers-which are taken into consideration as possible risk stratification markers). When studying physicians can make use imaging studies, such cardiac MRI, transthoracic (or transesophageal) echocardiography, coronary angiography, computed tomography, nuclear (which have been used cases where angiography was not possible), or even endomyocardial biopsy always available feasible). Two-case-series presentations: We present two positive male patients who were admitted Clinical Department Cardiology "Sfântul Apostol Andrei" Emergency Hospital Galați (Romania), presented distress symptoms diagnosed ST elevation AMI. 82 57 years old, respectively, moderate forms COVID-19, anteroseptal left ventricular extensive anterior transmural (with fibrillation at presentation), respectively. first patient a non-smoker non-drinker no associated comorbidities, later discharged, while second one died due to Conclusions: From this two-case series, we extract following: old age alone significant factor for adverse outcomes COVID-19-related events, that cumulative effects several patient-associated factors (be it either and/or injury) will most probably lead poor prognosis (death). At same time, enzymes, dynamic ECG changes, along developed echocardiographic modifications indicators regardless presence right dysfunction (due pulmonary hypertension).

Language: Английский

Citations

2
Multisystem Inflammatory Syndrome in Adults: Case Finding Through Systematic Review of Electronic Medical Records DOI Creative Commons
Michael Melgar, Julia C. Haston, Jennifer DeCuir

et al.

Clinical Infectious Diseases, Journal Year: 2022, Volume and Issue: 75(11), P. 1903 - 1911

Published: April 15, 2022

Multisystem inflammatory syndrome in adults (MIS-A) is a severe condition temporally associated with acute respiratory coronavirus 2 (SARS-CoV-2) infection.

Language: Английский

Citations

11
Comparative Evaluation of Rapid Isothermal Amplification and Antigen Assays for Screening Testing of SARS-CoV-2 DOI Creative Commons
Nol Salcedo, Brena F. Sena,

Xiying Qu

et al.

Viruses, Journal Year: 2022, Volume and Issue: 14(3), P. 468 - 468

Published: Feb. 25, 2022

Human transmission of SARS-CoV-2 and emergent variants concern continue to occur globally, despite mass vaccination campaigns. Public health strategies reduce virus spread should therefore rely, in part, on frequent screening with rapid, inexpensive, sensitive tests. We evaluated two digitally integrated rapid tests assessed their performance using stored nasal swab specimens collected from individuals or without COVID-19. An isothermal amplification assay combined a lateral flow test had limit detection 10 RNA copies per reaction, positive percent agreement (PPA)/negative (NPA) during the asymptomatic symptomatic phases 100%/100% 95.83/100%, respectively. Comparatively, an antigen-based 30,000 PPA/NPA 82.86%/98.68% 91.67/100%, Both optimized peak period transmission; however, reduced sensitivity clinical samples qPCR Ct values greater than 29.8. Low-cost, high-throughput enabled by techniques have value for outbreak control.

Language: Английский

Citations

11