Flow cytometry immune profiling of recurrent and newly diagnosed growth hormone secreting pituitary neuroendocrine tumors: comparison of two clinical cases

BMC Endocrine Disorders, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 12, 2025

Early detection of aggressive pituitary neuroendocrine tumors (PitNETs) remains challenging due to the absence reliable markers that can predict disease progression. Aggressive are typically identified through long-term observation. Tumor immune microenvironment (TIME) is crucial for understanding PitNETs' heterogeneity and identifying potential predictors tumor aggressiveness. In this study, we analyzed profile micro- macroenvironment in two somatotropinomas (aggressive non-aggressive) using flow cytometry. We observed lymphopenia elevated neutrophil levels periphery patient with recurrence. The TIME showed greater leukocyte infiltration, lymphoid cells predominating over myeloid along a higher proportion CD4 + T CD8 cells. detected double-positive population, high level regulatory sharp increase PD-1 expressing six months during blood recurrence, reduced granule-mediated cytotoxicity NK-cells. number effector producing perforin granzyme B—both independently simultaneously was significantly lower. CD3-CD20- NK CD3-CD56 low after first recurrence but increased sharply months. ratio monocyte subpopulations differed from blood, CD16 monocytes both patients. M2 macrophages rose 60.8% several immunological parameters, exhibited more pronounced immunosuppressive profile.

Language: Английский

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Overview of the 2022 WHO Classification of Pituitary Adenomas/Pituitary Neuroendocrine Tumors: Clinical Practices, Controversies, and Perspectives

Current Medical Science, Journal Year: 2022, Volume and Issue: 42(6), P. 1111 - 1118

Published: Dec. 1, 2022

Language: Английский

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Classifying Pituitary Adenoma Invasiveness Based on Radiological, Surgical and Histological Features: A Retrospective Assessment of 903 Cases

Journal of Clinical Medicine, Journal Year: 2022, Volume and Issue: 11(9), P. 2464 - 2464

Published: April 27, 2022

Invasiveness is a major predictor of surgical outcome and long-term prognosis in patients with pituitary adenomas (PAs). We assessed PA invasiveness via radiological, histological perspectives to establish classification scheme for predicting invasive behavior poor prognosis. retrospectively analyzed 903 who underwent transnasal-transsphenoidal surgery between January 2013 December 2019. Radiological (hazard ratio (HR) 5.11, 95% confidence interval (CI): 3.98–6.57, p < 0.001) (HR 6.40, CI: 5.09–8.06, better predicted gross-total resection (GTR) recurrence/progression-free survival (RPFS) rates than did 1.44, 1.14–1.81, = 0.003). Knosp grades 2 4.63, 2.13–10.06, 3 2.23, 1.39–3.59, 0.011) were predictors corresponding without invasiveness. Classifications 1 established based on invasiveness, respectively. Classification RPFS 1. Overall, radiological clinically valuable as prognostic predictors. The convenience good accuracy useful identifying PAs facilitating the development treatment plans.

Language: Английский

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Long-Term Functional and Tumor Outcomes Following Uni-Nostril Endoscopic Surgery for Non-Functioning Pituitary Adenomas: Results in 172 Patients

Neurology India, Journal Year: 2025, Volume and Issue: 73(1), P. 55 - 63

Published: Jan. 1, 2025

Background: Long-term functional and tumor outcomes in patients undergoing endoscopic surgery for non-functioning pituitary adenomas (NFPA) are not well documented. Objective: To study factors influencing the extent of resection (EOR) long-term visual, hormonal, following uni-nostril with nonfunctioning adenomas. Methods: In total 172 patients, ≥1 year follow-up, operated between 2005 2018 were included this retrospective study. Risk analyzed, logistic regression analysis, EOR visual hormonal outcomes. Recurrence rates correlated adjuvant radiation therapy (RT). Results: Gross (GTR) or near (NTR) was achieved 120 (69.7%) patients. Higher Knosp grade previous associated lower ( P = 0.02 0.004, respectively). On follow-up (mean, 72.5 months), impairment score improved 59.4% remained same 38.5% The axis 55.8% worsened 39.5% 10-year recurrence rate who had NTR 0% RT 63.2% without RT. Overall, non-RT group higher (51.2%) than (3.2%) 0.001). Conclusion: outcome NFPA is excellent good over 80% but most need life-long replacement therapy. control less a GTR, it leads to dysfunction.

Language: Английский

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Research Advances on Factors of Postoperative Recurrence of Pituitary Neuroendocrine Tumors

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(01), P. 2018 - 2025

Published: Jan. 1, 2025

Language: Английский

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Predictors of testosterone recovery in male patients with nonfunctioning pituitary adenoma treated with transnasal transsphenoidal extrapseudocapsular microsurgery

Metabolism and Target Organ Damage, Journal Year: 2025, Volume and Issue: 5(1)

Published: Feb. 17, 2025

Aim: To investigate the predictor of hypothalamic-pituitary-gonadal (HPG) axis recovery in male pituitary adenoma patients with trans-sphenoid extrapseudocapsular microsurgery. Methods: From June 2019 to December 2021, we retrospectively analyzed clinical data gonadal hormone changes and resection degree before after surgery nonfunctioning (NFPA) who underwent microsurgical by pseudocapsule technique same treatment group at Department Neurosurgery, Tongji Hospital. We explored predictors affecting postoperative testosterone using logistics regression analysis. Results: Among 291 adenomas, mean age was 50 years. Preoperative lower than normal 127 (43.6%); total subtotal were performed 279 (95.9%) 12 (4.1%), respectively. Postoperative follicle-stimulating (FSH) elevated 224 (77.0%) patients, luteinizing (LH) 230 (79.0%) prolactin significantly decreased 259 (89.0%) patients. Seventy-one low preoperative levels recovered levels. Univariate multivariate analysis suggested that tumor size, coagulative necrotic apoplexy (CNPA), invasiveness (P < 0.05). Conclusion: For NFPA, transsphenoidal extra-pseudocapsule microsurgery can effectively restore function anterior gland promote testosterone. Tumor CNPA

Language: Английский

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Tumor-Associated Fibroblast-Derived Exosomal circDennd1b Promotes Pituitary Adenoma Progression by Modulating the miR-145-5p/ONECUT2 Axis and Activating the MAPK Pathway

Cancers, Journal Year: 2023, Volume and Issue: 15(13), P. 3375 - 3375

Published: June 27, 2023

TAF participated in the progression of various cancers, including PA via release soluble factors. Exosomes belonged to extracellular vesicles, which were revealed as a crucial participator intercellular communication. However, expression pattern and effect TAF-derived exosomes remained largely unknown PA. In present study, we performed silico analysis based on public RNA-seq datasets generate circRNA/miRNA regulatory network. The qRT-PCR, Western blotting, RNA pull-down, luciferase assay investigate exosomes. exosomal circDennd1b was significantly upregulated promoted proliferation, migration, invasion cells sponging miR-145-5p cells. addition, directly regulated One Cut homeobox 2 (ONECUT2/OC2) inhibited promoting ONECUT2 We further demonstrated that transcriptionally increased fibroblast growth factor receptor 3 (FGFR3) expression, activates mitogen-activated protein kinases (MAPK) pathway, thus progression. Moreover, suppression TAFs by ABT-263 CSRM617 conclusion, our study illustrated affected regulating provides potential therapeutic strategy against aggressive

Language: Английский

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Diffusion-weighted imaging does not seem to be a predictor of consistency in pituitary adenomas

Pituitary, Journal Year: 2024, Volume and Issue: 27(2), P. 187 - 196

Published: Jan. 25, 2024

Language: Английский

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Endoscope-assisted transcranial surgery for complex pituitary adenoma: a retrospective case series study with propensity score matching

Neurosurgical Review, Journal Year: 2025, Volume and Issue: 48(1)

Published: Jan. 16, 2025

Language: Английский

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Radiological and Immunohistochemical Characteristics of PitNETs in 79 Patients Undergoing Neurosurgery

Cancers, Journal Year: 2025, Volume and Issue: 17(4), P. 666 - 666

Published: Feb. 16, 2025

The human pituitary is a gland located within small bony box, the sella turcica, under base of brain [...]

Language: Английский

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