Inhaled Exosomes Genetically Manipulated to Overexpress CD24 (EXO-CD24) as a Compassionate Use in Severe ARDS Patients

Biomedicines, Journal Year: 2023, Volume and Issue: 11(9), P. 2523 - 2523

Published: Sept. 13, 2023

Rationale: Acute respiratory distress syndrome (ARDS) is a major global health concern with significant unmet need. EXO-CD24 delivered via inhalation-reduced cytokines and chemokine secretion lung injury in ARDS improved survival mice models of ARDS, influenza, sepsis. Objectives: This clinical paper aims to evaluate the potential EXO-CD24, novel immunomodulatory treatment, compassionate care critically ill, intubated patients post-infection-induced acute (ARDS). Methods: Eleven ill diagnosed post-infection (10 COVID-19 one an adenovirus-associated infection) were administered four medical centers across Israel. The had multiple co-morbidities, including cancer, hypertension, diabetes, ischemic heart disease, met criteria for severe according Berlin classification. was inhalation, adverse events related its use carefully monitored. Measurements Main Results: administration did not result any recorded events. median hospitalization duration 11.5 days, overall mortality rate 36%. Notably, treated at Tel Aviv Medical Center (TASMC) showed lower 12.5%. WBC CRP levels decreased comparison baseline hospitalization, rapid responses occurred even kidney transplants who off ventilator within few days discharged shortly thereafter. production chemokines significantly suppressed all patients, those died. Among TASMC, on immunosuppressive drugs, them fully recovered from hospital. Conclusions: holds promise as therapeutic agent stages cases. Importantly, demonstrated favorable safety profile without apparent side effects promising efficacy. Furthermore, platform addressing hyper-inflammatory states warrants exploration. Further research larger-scale trials are warranted validate these preliminary findings.

Language: Английский

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Neuromuscular blockade and oxygenation changes during prone positioning in COVID-19

Journal of Critical Care, Journal Year: 2023, Volume and Issue: 79, P. 154469 - 154469

Published: Nov. 21, 2023

Language: Английский

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By activating endothelium histone H4 mediates oleic acid-induced acute respiratory distress syndrome

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 13, 2024

Objective: This study investigated pathogenic role and mechanism of extracellular histone H4 during oleic acid (OA)-induced acute respiratory distress syndrome (ARDS). Methods: ARDS was induced by intravenous injection OA in mice, evaluated blood gas, pathological analysis, lung edema, survival rate. Heparan sulfate (HS) degradation using immunofluorescence flow cytometry. The released von Willebrand factor (vWF) measured ELISA. P-selectin translocation neutrophil infiltration were via immunohistochemical analysis. Changes VE-cadherin western blot. Blocking antibodies against TLRs used to investigate the signaling pathway. Results: Histone plasma BALF increased significantly after injection. closely correlated with dose, which determined severity. Pretreatment further aggravated pulmonary edema death rate, while anti-H4 antibody exerted obvious protective effects. directly activated endothelia. Endothelial activation evidently manifested as HS degradation, release vWF, translocation, VE-Cadherin reduction. synergistic stimulus endothelia required for effective H4. Both calcium mediated H4-induced endothelial activation. Conclusions: Histone is an essential pro-inflammatory pro-thrombotic molecule OA-induced mice.

Language: Английский

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Adjoint Solver-Based Analysis of Mouth–Tongue Morphologies on Vapor Deposition in the Upper Airway

Fluids, Journal Year: 2024, Volume and Issue: 9(5), P. 104 - 104

Published: April 27, 2024

Even though inhalation dosimetry is determined by three factors (i.e., breathing, aerosols, and the respiratory tract), first two categories have been more widely studied than last. Both breathing aerosols are quantitative variables that can be easily changed, while airway morphologies difficult to reconstruct, modify, quantify. Although several methods available for model reconstruction modification, developing an anatomically accurate morphing it various physiological conditions remains labor-intensive technically challenging. The objective of this study explore feasibility using adjoint–CFD understand shape effects on vapor deposition control flux into lung. A mouth–throat was used, with mouth tongue being automatically varied via adjoint transport simulated ANSYS Fluent coupled a wall absorption model. Two chemicals varying adsorption rates, Acetaldehyde Benzene, were considered, which exhibited large differences in sensitivity shapes. For both chemicals, maximal possible identified then morphology parametric studies conducted. Results show changing mouth–tongue alter oral filtration 3.2% 0.27% Benzene under given condition. front exerts significant impact all cases other regions varies among cases. This demonstrates hybrid approach practical efficient method investigate morphology-associated variability vapors nanomedicines steady inhalation.

Language: Английский

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Assessment of lapachol's anti-inflammatory effectiveness in mitigating sepsis-induced acute lung injury

Fitoterapia, Journal Year: 2024, Volume and Issue: 180, P. 106298 - 106298

Published: Nov. 17, 2024

Language: Английский

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