Evaluation of loop-mediated isothermal amplification method for efficient detection of the periodontopathic bacteria Porphyromonas gingivalis DOI Creative Commons

Meenaz N. Sangolli,

Manohar S. Kugaji, Suman Kumar Ray

et al.

Journal of Indian Society of Periodontology, Journal Year: 2024, Volume and Issue: 28(1), P. 122 - 128

Published: Jan. 1, 2024

Abstract: Background: Periodontitis is a multifactorial, polymicrobial oral inflammatory illness brought on by pathogens. Porphyromonas gingivalis Gram-negative, obligatory anaerobic, black-pigmented coccobacillus and regarded as primary etiological factor in the progression of periodontitis. Rapid, highly senstitive specific detection methods are emerging. The present study aimed to evaluate loop-mediated isothermal amplification (LAMP) technique for efficiently detecting P . from subgingival plaque samples chronic periodontitis patients. Materials Methods: This included 50 patients suffering DNA (Deoxyribonucleic acid) was extracted “modified proteinase K” method. A set six primers, targeting pepO gene , used conducting LAMP. visualized naked-eye agarose electrophoresis. Conventional polymerase chain reaction (PCR) real-time qantitative PCR (qPCR) were carried out 16SrRNA (16S ribosomal ribonucleic Results: results showed that LAMP detected 40 (80%). Whereas, qPCR conventional P. 38 (76%) 33 (66%) respectively. sensitivity specificity method 94.87% 90.90%, With qPCR, found be 92.30% 81.81%, respectively, whereas, with PCR, it 76.92% 72.72%, Conclusion: an efficient quick, accurate, reliable identification samples. needs validated analytically, further studies can conducted taking saliva and/or gingival crevicular fluid

Language: Английский

Evaluation of loop-mediated isothermal amplification method for efficient detection of the periodontopathic bacteria Porphyromonas gingivalis DOI Creative Commons

Meenaz N. Sangolli,

Manohar S. Kugaji, Suman Kumar Ray

et al.

Journal of Indian Society of Periodontology, Journal Year: 2024, Volume and Issue: 28(1), P. 122 - 128

Published: Jan. 1, 2024

Abstract: Background: Periodontitis is a multifactorial, polymicrobial oral inflammatory illness brought on by pathogens. Porphyromonas gingivalis Gram-negative, obligatory anaerobic, black-pigmented coccobacillus and regarded as primary etiological factor in the progression of periodontitis. Rapid, highly senstitive specific detection methods are emerging. The present study aimed to evaluate loop-mediated isothermal amplification (LAMP) technique for efficiently detecting P . from subgingival plaque samples chronic periodontitis patients. Materials Methods: This included 50 patients suffering DNA (Deoxyribonucleic acid) was extracted “modified proteinase K” method. A set six primers, targeting pepO gene , used conducting LAMP. visualized naked-eye agarose electrophoresis. Conventional polymerase chain reaction (PCR) real-time qantitative PCR (qPCR) were carried out 16SrRNA (16S ribosomal ribonucleic Results: results showed that LAMP detected 40 (80%). Whereas, qPCR conventional P. 38 (76%) 33 (66%) respectively. sensitivity specificity method 94.87% 90.90%, With qPCR, found be 92.30% 81.81%, respectively, whereas, with PCR, it 76.92% 72.72%, Conclusion: an efficient quick, accurate, reliable identification samples. needs validated analytically, further studies can conducted taking saliva and/or gingival crevicular fluid

Language: Английский

Citations

0