Spectroscopic characterization of rosella flower extract (Hibiscus sabdariffa L.) and its antibacterial activity against Enterobacter aerogenes in suspected typhoid cases DOI Creative Commons

Siti Rohani,

Fadhil Rizki Martha, Trisnawati Mundijo

et al.

INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY, Journal Year: 2025, Volume and Issue: 7(1), P. 12 - 22

Published: April 9, 2025

Typhoid, a serious bacterial disease, has spurred research into natural products like rosella (Hibiscus sabdariffa L.) for potential treatments. This study investigates the chemical components found in Rosella extract using spectroscopy approximation, assisted by Fourier transform infrared (FTIR) and Gas chromatography–mass spectrometry (GC–MS). The antibacterial activity of on bacteria from stool cultures suspected typhoid cases continues to be evaluated. experimental employed post-test-only control group design, 30 μg Chloramphenicol as positive control, sterile distilled water negative at concentrations 25%, 50%, 75%, 100% observed variable. Stool samples patients were identified, Enterobacter aerogenes detected VITEK®2 testing. Cultivated tested determine extract. Phytochemical studies confirmed presence tannins, alkaloids, flavonoids, saponins Additionally, spectroscopic evaluation FTIR GC-MS showed groups, including esters, aldehydes, aromatics. Further clinical tests demonstrated minimum inhibitory concentration. results an increasing inhibition zone growth, correlating with increase Although was lower compared commercial Chloramphenicol, this product shows candidate future herbal medicine development.

Language: Английский

Spectroscopic characterization of rosella flower extract (Hibiscus sabdariffa L.) and its antibacterial activity against Enterobacter aerogenes in suspected typhoid cases DOI Creative Commons

Siti Rohani,

Fadhil Rizki Martha, Trisnawati Mundijo

et al.

INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY, Journal Year: 2025, Volume and Issue: 7(1), P. 12 - 22

Published: April 9, 2025

Typhoid, a serious bacterial disease, has spurred research into natural products like rosella (Hibiscus sabdariffa L.) for potential treatments. This study investigates the chemical components found in Rosella extract using spectroscopy approximation, assisted by Fourier transform infrared (FTIR) and Gas chromatography–mass spectrometry (GC–MS). The antibacterial activity of on bacteria from stool cultures suspected typhoid cases continues to be evaluated. experimental employed post-test-only control group design, 30 μg Chloramphenicol as positive control, sterile distilled water negative at concentrations 25%, 50%, 75%, 100% observed variable. Stool samples patients were identified, Enterobacter aerogenes detected VITEK®2 testing. Cultivated tested determine extract. Phytochemical studies confirmed presence tannins, alkaloids, flavonoids, saponins Additionally, spectroscopic evaluation FTIR GC-MS showed groups, including esters, aldehydes, aromatics. Further clinical tests demonstrated minimum inhibitory concentration. results an increasing inhibition zone growth, correlating with increase Although was lower compared commercial Chloramphenicol, this product shows candidate future herbal medicine development.

Language: Английский

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