Forests, Journal Year: 2023, Volume and Issue: 14(11), P. 2148 - 2148
Published: Oct. 28, 2023
Forests, often referred to as the lungs of our planet, stand a testament incredible diversity and significance terrestrial ecosystems [...]
Language: Английский
Forest Pathology, Journal Year: 2025, Volume and Issue: 55(2)
Published: March 12, 2025
ABSTRACT Phytophthora cinnamomi is one of the world's most invasive plant pathogens, requiring accurate detection in both and soil samples for effective disease management. This study compares existing qPCR assays with newly developed targeting internal transcribed spacer (ITS) gene improved detection. Our findings revealed that few current primers reliably distinguish P. from closely related species, even those did showed inadequate sensitivity. To address this, we designed novel a species‐specific probe ITS region, achieving consistent amplification limit 10 fg. The new assay successfully detected artificial symptomatic samples, ensuring rapid precise identification. optimised detects quantifies soil, providing superior sensitivity specificity over methods supporting more pathogen
Language: Английский
Citations
0
Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15
Published: Aug. 12, 2024
Phytophthora cinnamomi Rands devastates forest species worldwide, causing significant ecological and economic impacts. The European chestnut ( Castanea sativa ) is susceptible to this hemibiotrophic oomycete, whereas the Asian chestnuts crenata mollissima are resistant have been successfully used as resistance donors in breeding programs. molecular mechanisms underlying different disease outcomes among a key foundation for developing science-based control strategies. However, these still poorly understood. Dual RNA sequencing was performed C. roots inoculated with P. cinnamomi. studied time points represent pathogen’s lifestyle previously described at cellular level. expressed several genes related pathogenicity both species, such cell wall–degrading enzymes, host nutrient uptake transporters, effectors. expression of effectors modulation programmed death elicitins NLPs sporulation-related higher chestnut. After pathogen inoculation, 1,556 488 were differentially by , respectively. most transcriptional changes occur 2 h after inoculation (hai) 48 hai . Nevertheless, induced more defense-related genes, indicating that response controlled multiple loci, including pattern recognition receptors, involved phenylpropanoid, salicylic acid ethylene/jasmonic pathways, antifungal genes. Importantly, results validate observed responses Collectively, study provides comprehensive time-resolved description chestnut– dynamic, revealing new insights into important strategies development.
Language: Английский
Citations
2
Forests, Journal Year: 2023, Volume and Issue: 14(7), P. 1459 - 1459
Published: July 17, 2023
As an ornamental plant, Fatsia japonica has been widely used in gardens. From April 2021 to 2022, a disease that caused the wilting and root rot of F. large area was observed, which eventually led plants dying, while leaves did not fall off. This greatly reduced landscape effect plants. An oomycete species isolated from roots infected colony morphology slightly radial stellate, aerial mycelium flocculent. Oval sporangia with papillae, apical chlamydospores zoospores formed were observed. The morphological characteristics consistent Phytophthora. For accurate identification, internal transcribed spacer (ITS), cytochrome oxidase subunit II (COXII) ribosomal (LSU) genes amplified sequenced. identified as Phytophthora nicotianae using phylogenetic analysis. Finally, reproduced by inoculating healthy zoospore suspension; symptoms those natural infections, isolate obtained artificially had same inoculated isolate. results demonstrated P. is pathogenic factor rot. japonica. first report on China.
Language: Английский
Citations
4
Forests, Journal Year: 2024, Volume and Issue: 15(9), P. 1579 - 1579
Published: Sept. 9, 2024
Chestnut (Castanea sativa Mill.) forests in the Mediterranean region are facing increasing abandonment due to a combination of factors, ranging from climate change socioeconomic issues. The recovery chestnut ecosystems and their preservation valorization key ensuring supply wide spectrum ecosystem services they provide preventing detrimental environmental shifts. study’s objective was evidence on effects different management options ecophysiology forests, with diverse pruning intensities (low, medium, high intensity versus no pruning) tested an abandoned stand central Italy aim recovering rehabilitating it for fruit production. Innovative Internet Things (IoT) ‘Tree Talker’ devices were installed single trees continuously monitor measure ecophysiological (i.e., water transport, net primary productivity, foliage development) microclimatic parameters. Results show reduction use subjected medium- high-intensity treatments, along decrease carbon sequestration function. However, interestingly, results highlight that regain usual sap flow sink activity at end first post-pruning growing season fully realign during following year, as also confirmed by NDVI values. As such, this paper demonstrates efficacy managing initial setback resulting is rapidly remedied advantage reviving Additionally, reduced demand induced could represent promising adaptation strategy change, bolstering resilience prolonged intensified drought periods, which projected increase under future scenarios, particularly region.
Language: Английский
Citations
1
Acta Horticulturae, Journal Year: 2024, Volume and Issue: 1400, P. 77 - 88
Published: July 1, 2024
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Aug. 21, 2024
Abstract In recent decades an extensive mortality and decline of Quercus suber populations mainly caused by Phytophthora cinnamomi has been observed. the current study, a chestnut gene homologous to ginkbilobin-2 (Cast_ Gnk2-like ), which in Ginkgo biloba codifies antifungal protein, was transferred into cork oak somatic embryos three different embryogenic lines Agrobacterium mediated transformation. The transformation efficiency varied on genotype from 2.5 9.2%, total 22 independent transformed were obtained. presence Cast_Gnk2-like transgenic verified all PCR. number transgene copies estimated qPCR with high proliferation ability it between 1 5. addition, expression levels determined lines, higher derived ALM6-WT. Transgenic plants obtained evaluated after cold storage for 2 months subsequent transfer germination medium. vitro tolerance tests made under controlled conditions following zoospore treatment showed that overexpressing improved against Pc when compared wild type ones.
Language: Английский
Citations
0
Forests, Journal Year: 2023, Volume and Issue: 14(11), P. 2148 - 2148
Published: Oct. 28, 2023
Forests, often referred to as the lungs of our planet, stand a testament incredible diversity and significance terrestrial ecosystems [...]
Language: Английский
Citations
0