Functional Analysis of the ZmPR5 Gene Related to Resistance Against Fusarium verticillioides in Maize DOI Creative Commons
Wei Yang, Hongyu Cai, Yuanqi Zhang

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 737 - 737

Published: Feb. 28, 2025

In this study, the gene ZmPR5, associated with resistance to ear rot, was identified through transcriptome data analysis of maize inbred line J1259. The subsequently cloned and its function investigated. ZmPR5 comprises an open reading frame 525 base pairs, encoding a protein 175 amino acids. overexpressed in Arabidopsis ZmPR5EMS mutant maize, they were subjected q-PCR measurements antioxidant enzyme activities (POD, SOD, CAT, MDA), electrical conductivity, chlorophyll content. results indicate that expression is up-regulated upon infection by Fusarium verticillioides, significant differences observed POD, MDA, study found localized nucleus interacts Zm00001d020492 (WRKY53) Zm00001d042140 (glucA). Trypan blue staining revealed stained area significantly larger than B73. closely rot.

Language: Английский

Functional Analysis of the ZmPR5 Gene Related to Resistance Against Fusarium verticillioides in Maize DOI Creative Commons
Wei Yang, Hongyu Cai, Yuanqi Zhang

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 737 - 737

Published: Feb. 28, 2025

In this study, the gene ZmPR5, associated with resistance to ear rot, was identified through transcriptome data analysis of maize inbred line J1259. The subsequently cloned and its function investigated. ZmPR5 comprises an open reading frame 525 base pairs, encoding a protein 175 amino acids. overexpressed in Arabidopsis ZmPR5EMS mutant maize, they were subjected q-PCR measurements antioxidant enzyme activities (POD, SOD, CAT, MDA), electrical conductivity, chlorophyll content. results indicate that expression is up-regulated upon infection by Fusarium verticillioides, significant differences observed POD, MDA, study found localized nucleus interacts Zm00001d020492 (WRKY53) Zm00001d042140 (glucA). Trypan blue staining revealed stained area significantly larger than B73. closely rot.

Language: Английский

Citations

0