
International Journal of Food Science, Journal Year: 2024, Volume and Issue: 2024(1)
Published: Jan. 1, 2024
This study is aimed at investigating the reutilizing of gluten protein from wheat processing industry by Bacillus subtilis MTCC 2423 protease to obtain hydrolysates with high added value. Gluten hydrolysis using achieved a 34.07% degree 5% protein, time 2 h for 1000 U/mL pH 8.0 and temperature 40°C. Compared gluten, obtained exhibited enhanced functional attributes, including heightened solubility (43%), increased emulsifying activity (93.08 m /g), improved radical scavenging properties. Furthermore, these demonstrated antioxidant potential, as evidenced elevated ABTS (2,2 ′ ‐azino‐bis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) 81.25% DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) 56.46% activities also higher α ‐amylase inhibitory effect 33.98%. The enhancement in characteristics was observed Fourier transform infrared spectroscopy. percentage free amino acids protease‐mediated significantly compared unhydrolyzed wheat, which high‐performance liquid chromatography. These findings suggest that hold promising potential nutritional food ingredients industry, owing their functionalities antidiabetic
Language: Английский