Developing a separation system to enable real-time recovery of acetone-butanol during fermentation DOI Creative Commons

Christopher Chukwudi Okonkwo,

Ademola Duduyemi, Victor Ujor

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Nov. 9, 2024

Methods such as gas stripping and vacuum-assisted (VAGS) result in significant removal of water from the bioreactor, thus requiring continuous replenishment bioreactor. In this study, we developed a hydrophobic stainless steel meshes capable selectively recovering concentrated ABE stream bioreactor during VAGS. Three with pore sizes 180 µm, 300 425 µm were made oleophilic zinc oxide (ZnO) polydimethylsiloxane (PDMS). Butanol concentrations model solutions range 3 to 10 g/L which mimic typically produced batch fermentation. The integrated 5-L containing 2.5 L operational solution followed by evaluation selective extraction both cell-free Clostridium beijerinckii-rich solutions. results show that 180-µm ZnO/PDMS-coated mesh retained 54-64% more without C. beijerinckii cells 61-65% compared uncoated mesh. Furthermore, butanol concentration condensates recovered ZnO-PDMS-coated was up 10.8-fold greater than counterpart. Our data demonstrate ZnO-PDMS can recover high while retaining Additionally, technology demonstrates potential for real-time recovery fermentation lignocellulosic colloidal materials, concern clogging separation system. KEY POINTS: • Hydrophobic enhanced retention 1.65-fold. collected condensate increased 10.8-fold. is compatible lignocellulose.

Language: Английский

Developing a separation system to enable real-time recovery of acetone-butanol during fermentation DOI Creative Commons

Christopher Chukwudi Okonkwo,

Ademola Duduyemi, Victor Ujor

et al.

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Nov. 9, 2024

Methods such as gas stripping and vacuum-assisted (VAGS) result in significant removal of water from the bioreactor, thus requiring continuous replenishment bioreactor. In this study, we developed a hydrophobic stainless steel meshes capable selectively recovering concentrated ABE stream bioreactor during VAGS. Three with pore sizes 180 µm, 300 425 µm were made oleophilic zinc oxide (ZnO) polydimethylsiloxane (PDMS). Butanol concentrations model solutions range 3 to 10 g/L which mimic typically produced batch fermentation. The integrated 5-L containing 2.5 L operational solution followed by evaluation selective extraction both cell-free Clostridium beijerinckii-rich solutions. results show that 180-µm ZnO/PDMS-coated mesh retained 54-64% more without C. beijerinckii cells 61-65% compared uncoated mesh. Furthermore, butanol concentration condensates recovered ZnO-PDMS-coated was up 10.8-fold greater than counterpart. Our data demonstrate ZnO-PDMS can recover high while retaining Additionally, technology demonstrates potential for real-time recovery fermentation lignocellulosic colloidal materials, concern clogging separation system. KEY POINTS: • Hydrophobic enhanced retention 1.65-fold. collected condensate increased 10.8-fold. is compatible lignocellulose.

Language: Английский

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