Lactate production from lactose-rich wastewater: A comparative study on reactor configurations to maximize conversion rates and efficiencies DOI Creative Commons
Monika Temovska,

Richard Hegner,

Andrés E. Ortiz-Ardila

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

ABSTRACT About 90% of global lactate production is derived from bacterial fermentation sugars via pure cultures homofermentative bacteria in batch mode. Acid whey, which a wastewater the yogurt industry with lactose and galactose as main sugars, can be used an alternative substrate for commercial lactate. Operating open microbial consortia ( i.e ., reactor microbiomes) reduces costs by circumventing sterilization, while continuous operation achieves higher productivity at shorter times. Homofermentation achieved maintaining acidic thermophilic conditions, product formation systems increased biomass retention strategies. To find best configuration acid we operated three different configurations: (1) upflow anaerobic sludge blanket (UASB) reactor; (2) filter (AFR); (3) continuously stirred tank (CSTR) hollow-fiber membrane module. We hydraulic times (HRTs) to optimum parameters maximize galactose-into-lactate (LG-into-LA) conversion efficiency. did not use inoculum but enriched endogenous D-lactate-producing Lactobacillus spp. that later dominated microbiomes (> relative abundance). Undissociated lactic concentrations more than 60 mmol C L -1 inhibited microbiomes. alleviated inhibition effect shortening HRT 0.6 days using diluted acid-whey (1.67-fold dilution) achieve almost complete At 0.6-day commencement, AFR CSTR performed better UASB due their cell abilities. During period between Day 365-384, experienced error pH control system during value dropped 4.3. After this pH-error period, LG-into-LA efficiency considerably improved surpassed AFR. highest rate 1256 ± 46.3 d (1.57 0.06 g h ) 82.2 3.4% (in C), yield 0.85 0.02 (product per consumed substrate) CSTR.

Language: Английский

Lactate production from lactose-rich wastewater: A comparative study on reactor configurations to maximize conversion rates and efficiencies DOI Creative Commons
Monika Temovska,

Richard Hegner,

Andrés E. Ortiz-Ardila

et al.

Water Research, Journal Year: 2025, Volume and Issue: 278, P. 123365 - 123365

Published: Feb. 22, 2025

Language: Английский

Citations

0
Lactate production from lactose-rich wastewater: A comparative study on reactor configurations to maximize conversion rates and efficiencies DOI Creative Commons
Monika Temovska,

Richard Hegner,

Andrés E. Ortiz-Ardila

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

ABSTRACT About 90% of global lactate production is derived from bacterial fermentation sugars via pure cultures homofermentative bacteria in batch mode. Acid whey, which a wastewater the yogurt industry with lactose and galactose as main sugars, can be used an alternative substrate for commercial lactate. Operating open microbial consortia ( i.e ., reactor microbiomes) reduces costs by circumventing sterilization, while continuous operation achieves higher productivity at shorter times. Homofermentation achieved maintaining acidic thermophilic conditions, product formation systems increased biomass retention strategies. To find best configuration acid we operated three different configurations: (1) upflow anaerobic sludge blanket (UASB) reactor; (2) filter (AFR); (3) continuously stirred tank (CSTR) hollow-fiber membrane module. We hydraulic times (HRTs) to optimum parameters maximize galactose-into-lactate (LG-into-LA) conversion efficiency. did not use inoculum but enriched endogenous D-lactate-producing Lactobacillus spp. that later dominated microbiomes (> relative abundance). Undissociated lactic concentrations more than 60 mmol C L -1 inhibited microbiomes. alleviated inhibition effect shortening HRT 0.6 days using diluted acid-whey (1.67-fold dilution) achieve almost complete At 0.6-day commencement, AFR CSTR performed better UASB due their cell abilities. During period between Day 365-384, experienced error pH control system during value dropped 4.3. After this pH-error period, LG-into-LA efficiency considerably improved surpassed AFR. highest rate 1256 ± 46.3 d (1.57 0.06 g h ) 82.2 3.4% (in C), yield 0.85 0.02 (product per consumed substrate) CSTR.

Language: Английский

Citations

0