Effect of nitrogen ((NH4)2SO4) starvation on growth, lipids, and characterization of fatty acid methyl esther (FAME) in Euglena sp.: Semi-mass cultivation DOI Open Access
Eko Agus Suyono, Sarwat Zahra,

Tariq Akhdan Purnama

et al.

Asia-Pacific Journal of Molecular Biology and Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 139 - 150

Published: Dec. 31, 2024

Microalgae, such as Euglena sp., are microorganisms that can be utilized biofuel sources. Before mass cultivation, sp. must adapt through semi-mass cultivation to tolerate various conditions, including environmental changes and contaminants. Nitrogen starvation has been shown increase lipid production in sp.; however, the effects of culturing with ((NH4)2SO4) concentrations 500 mg/L 250 have not yet explored. This research aimed determine on growth rate biomass, content, fatty acid profile during (50 L). In this study, nitrogen increased total unsaturated acids, production, productivity. However, it reduced cell growth, biomass The highest productivity was observed at ((NH4)2SO4), reaching 0.011±0.0014 mg/mL 0.217±0.014 for day 15. Meanwhile, occurred 1000 0.156±0.010 15 a 0.0067 mg/mL. Methyl palmitoleate (C16:1) dominant FAME all treatments, 18.38% ((NH4)2SO4). Thus, concluded treatment is more efficient increasing FAME, production.

Language: Английский

Sustainable valorization of waste glycerol into bioethanol and biodiesel through biocircular approaches: a review DOI Creative Commons
Mahdy Elsayed,

Mohamed Eraky,

Ahmed I. Osman

et al.

Environmental Chemistry Letters, Journal Year: 2023, Volume and Issue: 22(2), P. 609 - 634

Published: Dec. 1, 2023

Abstract Liquid biofuels like biodiesel and bioethanol are crucial in the transition to low-carbon high-energy alternatives fossil fuels. One significant by-product of production is glycerol, which accounts for about 10% total conversion output. While waste glycerol poses challenges due its impurities contaminants, it also holds potential as a metabolic resource essential cellular components microorganisms. Crude reviewed, highlighting relevance current technologies biochemical composition. To efficiently utilize co-valorization with low-cost substrates through biocircular platforms using various microorganisms or insects second third-generation oxy-biofuels has been explored. Among these, black soldier fly larvae have demonstrated higher competitiveness lipid contents (35–43%), making them promising organism recycling into production, alongside microalgae oleaginous yeast. The microbial productivity from yeast notably (3546 kg ha −1 y ) than soybean (562 ), while microalgal surpasses palm by more 25 times. Remarkably, was reported be ~ 1.7 times an impressive 43 biodiesel. Despite their industry still represents challenge because high impurities, viscosity, limited direct applications existing processes. further enhance energy sustainability address discussed utilization domestic wastewater sludge, lignocellulosic biomass, protein-rich wastes. These offer opportunities create other sustainable agricultural products minimizing environmental footprint.

Language: Английский

Citations

27
Efficient bioflocculation and biodiesel production of microalgae Asterococcus limneticus on streptomyces two-stage co-cultivation strategy DOI
Manogaran Lakshmikandan,

A.G. Murugesan,

Fuád Ameén

et al.

Biomass and Bioenergy, Journal Year: 2023, Volume and Issue: 175, P. 106886 - 106886

Published: June 26, 2023

Language: Английский

Citations

11
Conversion of protein-rich waste into biodiesel by Hermetia illucens: Enhanced energy recovery and reduced greenhouse gas emissions DOI
Mahdy Elsayed, Jing Wang, Huijun Wang

et al.

Sustainable Energy Technologies and Assessments, Journal Year: 2024, Volume and Issue: 66, P. 103825 - 103825

Published: May 25, 2024

Language: Английский

Citations

4
Enhancing nutrient removal from hydroponic effluent with simultaneous production of lipid-rich biomass through mixotrophic cultivation of microalgae DOI

Rayanee Chaudhuri,

P. Balasubramanian

Journal of Applied Phycology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Language: Английский

Citations

0
N:P ratio and salinity as keys: A study on optimizing biomass and lipid production in marine Chlorella sp. NITT 02 and Picochlorum sp. NITT 04 for biodiesel production DOI
Susaimanickam Anto,

M. Premalatha,

Thangavel Mathimani

et al.

Biomass and Bioenergy, Journal Year: 2024, Volume and Issue: 190, P. 107409 - 107409

Published: Oct. 4, 2024

Language: Английский

Citations

2
Lipid Enhancement in Oleaginous Nannochloropsis sp. under Nitrate Limitation for Future Bioenergy Production DOI Creative Commons
Karthick Murugan Palanisamy, Prakash Bhuyar, Mohd Hasbi Ab. Rahim

et al.

International Journal of Energy Research, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 8

Published: Dec. 14, 2023

Microalgae possess high oil content, exhibit rapid growth rates and biomass productivity, leave a minimal environmental footprint, making them highly attractive for biofuel applications. Consequently, this present study is aimed at evaluating the impact of nitrogen starvation on marine microalgae Nannochloropsis sp. to enhance lipid accumulation. The microalga culture was grown under an irradiance 120 μmol photon m-2 s-1, temperature 25 ± 1 °C, continuous air-CO2, with different concentrations sodium nitrites over 14-day cultivation period. From results, it found that strain decreased limited conditions. After harvesting, cells in supernatant were regenerated by cultivating double concentration media. results showed 12th day, highest content achieved, reaching id="M2"> 68.0 2.3 wt.% dry weight limitation. composition obtained consisted 18.0 saturated fatty acids, 72.38 monounsaturated 8.95 polyunsaturated acids. dominated oleic acid ( id="M3"> 70.97 4.6 wt.%), palmitic id="M4"> 13.68 1.0 linoleic id="M5"> 8.41 0.4 wt.%). findings demonstrated limiting availability enhanced accumulation Despite experiencing inhibited growth, species shows great potential large-scale lipids essential These contribute understanding relationship between metabolism microalgae, providing valuable insights optimizing production context biotechnology

Language: Английский

Citations

6
A Response Surface Methodology Study for Chlorella vulgaris Mixotrophic Culture Optimization DOI Creative Commons

Sandra Milena Rincón,

Haluk Beyenal, Hernán Mauricio Romero

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(2), P. 379 - 379

Published: Feb. 12, 2024

Glycerol is a carbon source that produces good biomass under mixotrophic conditions. Enhancing the composition of culture media in algae production improves growth rates, yield, nutrient utilization efficiency, and overall cost-effectiveness. Among key nutrients medium, nitrogen plays pivotal role. Urea can be effectively used as considered low-cost form compared to other sources. metabolism releases some CO2 photosynthesis, magnesium major role urea uptake. Magnesium another photosynthesis metabolic reactions. To maximize glycerol consumption system obtain high lipid productions, variations MgSO4·7H2O concentrations were evaluated medium microalgae. A response surface methodology (RSM) using central composite design (CCD) was designed at initial cellular rates (up four days). The supply varied from 0.3 1.7 g L−1. Response utilized analyze results, highest rate, 770.2 mg L−1 d−1, observed when C. vulgaris grown urea, 1.0 MgSO4·7H2O. Using optimal with acetate, glucose, sources, same content (10% average) achieved on day 4 culture. Overall, results show an optimum concentration yields large amounts fatty acids greatly influences profile acids.

Language: Английский

Citations

1
Microalgal lipid production for fatty acid methyl esters conversion: A response surface methodology optimisation of media nutrient in batch reactors DOI Creative Commons
Kevin Nyoni,

Leungo Kelebopile,

Lesedi Lebogang

et al.

Results in Chemistry, Journal Year: 2024, Volume and Issue: 9, P. 101668 - 101668

Published: July 1, 2024

Biodiesel has the potential to reduce heavy dependence on petroleum diesel, and microalgae are considered a feedstock, owing their ability produce high lipids. In this study, effect of brine salinity cell growth lipid production was investigated. Brine 2.50 M NaCl selected as optimum for biomass (2480 ± 75 mgL−1), with content (18.00 %), it further used in nutrient optimization processes by Response Surface Methodology (RSM). The Plackett-Burman Box-Behnken designs RSM were select optimize significant medium nutrients culturing Dunalliela salina maximum production. method identified NaHCO3, NaH2PO4·2H2O NaNO3 concentration, productivity. While design optimized developed model (R2 = 95.80 %) that yielded 181.10 mgL−1, which 12.30 % improvement under culture comprising 1302.4 mgL−1 22.5 NaH2PO4·2H2O, 181.1 NaNO3, 44.7 CaCl2, 2355.0 MgSO4·7H2O, 5.6 mlL−1 micronutrients solution. extracted lipids methanol-transesterified, GC–MS analysis revealed fatty acid methyl esters carbon chains between C14 C20. dominated 8-octadecenoic (36.69 hexadecanoic (35.53 9-octadecenoic Z (17.85 signifying characteristics good quality biodiesel. Therefore, D. interaction is highly feasible source sustainable renewable biodiesel scaled up

Language: Английский

Citations

0
Effect of nitrogen ((NH4)2SO4) starvation on growth, lipids, and characterization of fatty acid methyl esther (FAME) in Euglena sp.: Semi-mass cultivation DOI Open Access
Eko Agus Suyono, Sarwat Zahra,

Tariq Akhdan Purnama

et al.

Asia-Pacific Journal of Molecular Biology and Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 139 - 150

Published: Dec. 31, 2024

Microalgae, such as Euglena sp., are microorganisms that can be utilized biofuel sources. Before mass cultivation, sp. must adapt through semi-mass cultivation to tolerate various conditions, including environmental changes and contaminants. Nitrogen starvation has been shown increase lipid production in sp.; however, the effects of culturing with ((NH4)2SO4) concentrations 500 mg/L 250 have not yet explored. This research aimed determine on growth rate biomass, content, fatty acid profile during (50 L). In this study, nitrogen increased total unsaturated acids, production, productivity. However, it reduced cell growth, biomass The highest productivity was observed at ((NH4)2SO4), reaching 0.011±0.0014 mg/mL 0.217±0.014 for day 15. Meanwhile, occurred 1000 0.156±0.010 15 a 0.0067 mg/mL. Methyl palmitoleate (C16:1) dominant FAME all treatments, 18.38% ((NH4)2SO4). Thus, concluded treatment is more efficient increasing FAME, production.

Language: Английский

Citations

0