Upper
mesophilic
temperature
acclimation
of
halophilic,
hydrogen-producing
bacteria
from
salt
fields
was
investigated
in
this
study,
along
with
the
changes
microbial
abundance
during
an-aerobic
digestion
(AD)
process.
Genomic
approaches
such
as
PCR-denaturing
gradient
gel
elec-trophoresis
(DGGE)
and
next-generation
sequencing
(NGS)
were
performed
to
profile
mi-crobial
communities.
During
AD,
there
a
significant
Halanaerobacter
lacunarum
at
48°C
followed
by
increase
hydrogen
yield,
signifying
potential
contribution
halophile
production.
A
decrease
dominance
H.
Halan-aerobium
fermentans
42°C,
likely
due
an
other
bacterial
species,
noted
but
their
significantly
increased
temperatures
45°C
48°C.
This
investigation
provides
valuable
insights
highlighting
Halanaerobium
sp.
halo-philic
adapt
under
upper
conditions
synthesizing
hy-drogen.
The
findings
present
study
also
underscore
importance
optimizing
temper-ature
pH
maximize
yield
high-salt
anaerobic
digestion.
Biotechnology Notes,
Journal Year:
2024,
Volume and Issue:
5, P. 33 - 49
Published: Jan. 1, 2024
The
excessive
discharge
and
accumulation
of
solid
organic
waste
into
the
environment
is
severe
concern
across
globe.
Thus,
an
efficient
management
system
important
to
mitigate
health
risks
humans,
minimize
harmful
impacts
on
environment,
ensure
a
sustainable
ecosystem.
converted
value-added
products
either
using
microorganisms
or
heat
energy;
these
methods
are
commonly
known
as
biochemical
thermochemical
techniques.
process
has
advantage
higher
selectivity
lower
processing
temperatures.
principal
conversion
processes
this
category
fermentation
anaerobic
digestion
(AD).
This
review
article
focuses
AD,
potential
method
for
treating
creating
variety
with
added
value.
Here
we
present
digestibility
various
wastes,
role
microorganisms,
decomposition
process,
co-substrates,
digester
designs,
biogas
yields,
by-products,
environmental
impacts,
overall
techno-economical
effectiveness
process.
Further,
offers
insights
new
directions
AD
treatment
future
research
without
compromising
feasibility
sustainability.
Water Cycle,
Journal Year:
2024,
Volume and Issue:
5, P. 146 - 155
Published: Jan. 1, 2024
Short-time
aerobic
digestion
(STAD)
of
sludge
requires
low
investment
and
has
the
advantages
short
retention
time
rapid
degradation
organic
matter
in
waste
activated
(WAS).
This
research
conducted
STAD
at
three
temperature
gradients
(15°C,
25°C,
35°C).
Characteristics
EPS
microbial
community
structure
raw
fermented
were
analyzed,
environmental
application
potential
different
temperatures
was
explored.
treatment
improved
both
polysaccharide
(PS)
protein
(PN)
contents
EPS,
with
increases
5.7‒18.35%
20.98‒28.57%,
respectively.
Aromatic
protein-like
substances
(Peak
E)
tryptophan
F)
main
components
PN
EPS.
The
species
richness
decreased
as
increased.
At
class
level,
significantly
affected
Alphaproteobacteria,
Gemm-1,
Chloracidobacteria,
Acidimicrobiia,
Ellin6529,
Betaproteobacteria,
SJA-28.
From
an
perspective,
specific
resistance
to
filtration
(SRF)
value
WAS
after
35°C
7.01×107
m/kg
a
17.34%
reduction,
indicating
improvement
dewaterability
sludge.
Pb2+
adsorption
efficiency
per
unit
volume
increased
by
10.0‒16.2%
for
four
hours.
elucidated
impact
on
systems,
finding
that
abundance
correlated
characteristics.
results
provide
basis
optimizing
system
systems
deeper
understanding
benefits
practical
applications
resource
utilization.
Environmental Science and Ecotechnology,
Journal Year:
2024,
Volume and Issue:
21, P. 100440 - 100440
Published: June 13, 2024
Anaerobic
digestion
(AD)
plays
a
significant
role
in
renewable
energy
recovery.
Upgrading
AD
from
thermophilic
(50–57
°C)
to
mesophilic
(30–38
conditions
enhance
process
stability
and
reduce
input
remains
challenging
due
the
high
sensitivity
of
microbiomes
temperature
fluctuations.
Here
we
compare
effects
two
decreasing-temperature
modes
55
35
°C
on
cell
viability,
microbial
dynamics,
interspecies
interactions.
A
sharp
transition
(ST)
is
one-step
by
20
d−1,
while
mild
(MT)
stepwise
1
d−1.
We
find
greater
decrease
methane
production
with
ST
(88.8%)
compared
MT
(38.9%)
during
period.
mode
overproduced
reactive
oxygen
species
1.6-fold,
increased
membrane
permeability
2.2-fold,
downregulated
metabolism
25.1%,
leading
apoptosis
anaerobes
1.9-fold
release
intracellular
substances
2.9-fold,
further
constraining
methanogenesis.
The
higher
(1.6
vs.
1.1
copies
per
gyrA)
metabolic
activity
acetate-dependent
methanogenesis
implied
more
efficient
steady
mesophilic,
MT-mediated
system.
Metagenomic
binning
network
analyses
indicated
that
induced
dysbiosis
keystone
greatly
enhanced
functional
redundancy,
causing
loss
syntrophic
interactions
redundant
pathways.
In
contrast,
interconnections
(average
degrees
44.9
22.1)
at
state
suggested
could
better
maintain
necessary
system
functionality
through
syntrophy
or
specialized
Adopting
transform
digesters
into
feasible
potentially
optimization
broader
application
practical
anaerobic
engineering.
Energies,
Journal Year:
2023,
Volume and Issue:
16(17), P. 6343 - 6343
Published: Sept. 1, 2023
Hydrogen
as
an
energy
vector
is
going
to
play
important
role
in
the
global
mix.
On
other
hand,
wastewater
management
has
become
a
worldwide
concern,
urban
settlements
have
been
considerably
increasing
for
decades.
Consequently,
biodigestion
produce
biogas
(rich
methane)
water
treatment
plants
could
be
interesting
starting
point
obtain
valuable
gas
that
can
converted
into
hydrogen
through
steam
reforming.
The
aim
of
this
work
was
review
main
aspects
concerning
reforming
from
plants.
For
purpose,
whole
chain,
production
and
purification,
considered,
paying
attention
challenges
new
technologies
its
optimization.
Thus,
wide
range
possibilities
offered,
direct
use
syngas
high
purification
(mainly
pressure
swing
adsorption
or
membrane
reactors),
presenting
advantages
disadvantages.
In
any
case,
catalysts
seems
essential,
such
sulfide
coke
deposition
control
should
addressed.
conclusion,
applied
reality,
with
serious
implementation
at
industrial
level,
according
techno-economic
assessment.
Fermentation,
Journal Year:
2025,
Volume and Issue:
11(3), P. 108 - 108
Published: Feb. 24, 2025
The
growth
and
metabolism
of
photosynthetic
bacteria
play
a
significant
role
in
the
efficiency
substrate
energy
conversion
photo-fermentation
biohydrogen
production
(PFHP).
In
this
paper,
influence
different
concentrations
L-alanine
(0.3–1.2
g/L),
L-leucine
(0.6–1.5
L-serine
(1–2.5
g/L)
on
PFHP
microbial
were
investigated.
results
showed
that
sole
additions
at
0.6
g/L,
0.9
1.5
g/L
to
could
enhance
cumulative
260
±
4.01
mL
(39.04%
increase),
267
4.27
(42.78%
248
3.97
(32.62%
respectively.
An
analysis
scatter
matrix
plots
indicated
three
amino
acid
additives
key
increasing
hydrogen
production.
This
study
helps
further
explore
effect
acid-based
PFHP.
Energies,
Journal Year:
2024,
Volume and Issue:
17(2), P. 364 - 364
Published: Jan. 11, 2024
Biogas
is
a
renewable
energy
source
that
comes
from
biological
waste.
In
the
biogas
generation
process,
various
factors
such
as
feedstock
composition,
digester
volume,
and
environmental
conditions
are
vital
in
ensuring
promising
production.
Accurate
prediction
of
yield
crucial
for
improving
operation
increasing
yield.
The
purpose
this
research
was
to
propose
novel
approach
improve
accuracy
predicting
using
stacking
ensemble
machine
learning
approach.
This
integrates
three
algorithms:
light
gradient-boosting
(LightGBM),
categorical
boosting
(CatBoost),
an
evolutionary
strategy
attain
high
performance
accuracy.
proposed
model
tested
on
data
collected
production
facilities.
It
employs
optimum
parameter
selection
ensembles
showed
better
variability.
A
comparative
analysis
with
others
k-nearest
neighbor
(KNN),
random
forest
(RF),
decision
tree
(DT)
performed.
study’s
findings
demonstrated
outperformed
existing
models,
root-mean-square
error
(RMSE)
0.004
mean
absolute
(MAE)
0.0024
metrics.
conclusion,
accurate
predictive
cooperating
fermentation
control
system
can
significantly
increase
stands
pivotal
step
toward
meeting
escalating
global
demands.
