The protective effect of freezing temperatures on different lactic acid bacteria and its mechanism DOI Creative Commons
Pin Chen, Wen Tan, Huan Cheng

et al.

LWT, Journal Year: 2024, Volume and Issue: unknown, P. 117226 - 117226

Published: Dec. 1, 2024

Language: Английский

Investigation on the physicochemical properties and volatile components of egg yolk fermented by different lactic acid bacteria and quality evaluation of mayonnaise prepared from fermented egg yolk DOI
Jiahao Bie, Wendi Teng,

Zhiguo Peng

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: 66, P. 106261 - 106261

Published: Feb. 28, 2025

Language: Английский

Citations

0
Complete genome sequence, metabolic profiling and functional studies reveal Ligilactobacillus salivarius LS-ARS2 is a promising biofilm-forming probiotic with significant antioxidant, antibacterial, and antibiofilm potential DOI Creative Commons

Sinjini Patra,

Biswaranjan Pradhan, Anasuya Roychowdhury

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 20, 2025

Background Probiotics restore microbial balance and prevent gut-inflammation. Therefore, finding out novel probiotic strains is a demand. As gut-microbe, benefits of Ligilactobacillus salivarius (LS) are established. However, strain-specific detailed studies limited. Here, we illustrate attributes LS-ARS2 for its potential application as food-supplement and/or therapeutic to improve gut-health. Methods Whole genome sequencing (WGS) phylogenetic analysis confirm the strain LS . To establish properties, acid-bile tolerance, auto-aggregation, cell-surface-hydrophobicity, biofilm-formation, adhesion-assays performed. ensure safety attributes, antibiotic-susceptibility, hemolytic, DNase, trypan-blue, MTT assays done. ABTS, DPPH, superoxide, hydroxyl free radical scavenging used determine anti-oxidant potential. Antibacterial assays, including co-culture assay with pathogen pathogenic biofilm-inhibition performed explore antibacterial efficacy. characterize metabolic-profile -derived cell-free-supernatant (CFS), HRMS carried out. Consequently, WGS-analyses predict molecular associations related functional outcomes. Results We find remarkable fast-growing that shows acid bile tolerance (>60% survival rate), indicating promising gut-sustainability. High auto-aggregation capacity (>80%), robust cell-surface hydrophobicity (>85%), adhesion efficacy Caco-2 cells significant gut colonization. Fascinatingly, able form biofilm within 24 h ( p < 0.0001), rare among strains, efficient stay in gut. The ensures attributes. -WGS recognizes probiotic-specific determinants, predicts genomic stability, identifies orthologous-clusters diverse functions, metabolites bacteriocins. HRMS-studies LS-ARS2- CFS further validate presence beneficial antimicrobial immunomodulatory antioxidant properties ABTS (>60%), DPPH (>10 U/mL), superoxide (>70%), (>70%). Further, activities against Gram-positive Methicillin-resistant Staphylococcus aureus (MRSA) Gram-negative multidrug-resistant clinical enterotoxigenic Escherichia coli, Vibrio cholerae, Shigella flexneri Anti- Salmonella effect prominent 0.0001). Most interestingly, -CFS inhibits MRSA -biofilm again strains. Conclusion novel, fast-growing, biofilm-forming antioxidant, antibacterial, anti-biofilm potentials, suggesting applications combating biofilms improving vivo would facilitate their applications.

Language: Английский

Citations

0
Qualitative and quantitative detection of the quorum sensing signalling molecule autoinducer-2 in fermented milk based on 2,3-diaminonaphthalene derivatisation DOI

Hanyi Li,

Zihang Shi,

Xiankang Fan

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106420 - 106420

Published: March 1, 2025

Language: Английский

Citations

0
Reference Whole Genome Sequence Analyses and Characterization of a Novel Carnobacterium maltaromaticum Distinct Sequence Type Isolated from a North American Gray Wolf (Canis lupus) Gastrointestinal Tract DOI Creative Commons
C. Cristoph Klews, J. Bryant, Jennifer A. McCabe

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(5), P. 410 - 410

Published: April 27, 2025

We hypothesize that bacteria isolated from free-ranging animals could potentially be useful for practical applications. To meet this objective a Gram-positive bacterium was the gastrointestinal (GI) tract of Gray Wolf (Canis lupus) using Brucella broth with hemin and vitamin K (BBHK). By small ribosomal RNA (16S) gene sequencing initially identified as novel Carnobacterium maltaromaticum strain. The propagated both anaerobically aerobically catalase/oxidase negative by starch hydrolysis well lipase assays. reference whole genome sequence (WGS) obtained Illumina Nanopore sequencing. assembly 3,512,202 bp in length, encoding core bacterial genes GC% content 34.48. No lysogenic bacteriophage were detected, although harbors expression bacteriocin other secondary metabolites potential antimicrobial properties. Multilocus typing (MLST), WGS phylogenetics, average nucleotide identity (ANI), single polymorphism (SNP) analyses isolate’s indicate is newly type (ST). Members Carnobacteria have anti-listeria activities, highlighting their functional Consequently, isolate probiotic canids first report on an axenic C. culture genus Canis.

Language: Английский

Citations

0
Functional and molecular characterization of millet associated probiotic bacteria DOI Creative Commons

Bashipangu Gouthami,

A. Ramalakshmi,

M. Balakrishnan

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 20, 2024

The lactic acid bacteria are one of the sustainable ways food production. As native (LAB) easily manipulate substrate, helps in production health essential probiotics with enhancing bioavailability substrate. Here also, present study, LAB isolates isolated from millets and characterize them for functional analysis human association. In fermented millet-associated were screened characterized their probiotic potential, safety evaluation antimicrobial activity. A total 33 purified as based on colony shape biochemical assays. However, only 13 found to be catalase-negative. Among isolates, 5 exhibited optimum growth at 6.5% 9.5% salt concentrations, pH 4.5 8.5 17 °C 40 temperature. properties five that survival rates bile concentration ranged 56.2 73.7% 55.3 70.3%, respectively. Similarly, surface hydrophobicity was 41–75%. Antibiotic assay revealed all resistant Amoxicillin, Cloxacillin, Penicillin-V. Interestingly, except ME26 displayed susceptibility towards Penicillin (2 units) Tetracycline (10 µg). Further, four (ME25, ME26, ME9, ME2) had more antifungal activity against Aspergillus flavus. three, ME1 ME2, showed maximum antibacterial produced compounds compared reference strain L. plantarum Pb3. potential identified Weisella cibaria confusa ME25.

Language: Английский

Citations

3
Metabolic profile and antioxidant properties of grape juice subjected to long-fermentation with Lactiplantibacillus plantarum strain isolated from traditional pickled vegetables DOI

Li Yanlu,

Wenting Fu,

Han-chang Du

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: 62, P. 105455 - 105455

Published: Nov. 13, 2024

Language: Английский

Citations

1
Metagenomic Insights into Enhancing Protein Content and Digestibility in Jack Bean (Canavalia Ensiformis) Tempeh: Unraveling Microbial Dynamics During Fermentation DOI

STP. Vira Putri Yarlina,

Mohammad Djali,

Robi Andoyo

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Fermentation with Lactic Acid Bacteria for Bean Flour Improvement: Experimental Study and Molecular Modeling as Complementary Tools DOI Creative Commons
Carlos Sabater, Gabriel Dario Saez,

Nadia E. Suárez

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(13), P. 2105 - 2105

Published: July 2, 2024

Pulses are considered superfoods for the future world due to their properties, but they require processing reduce antinutritional factors (ANFs) and increase bioactivity. In this study, bean flour (Phaseolus vulgaris L.) was fermented under different conditions (addition of Lactiplantibacillus plantarum CRL 2211 and/or Weissella paramesenteroides 2182, temperature, time dough yield) improve its nutri-functional quality. Fermentation 24 h at 37 °C with mixed starter increased lactic acid bacteria (LAB) population, acidity, polyphenol content (TPC) ANF removal more than spontaneous fermentation. Statistical rep-PCR analysis showed that fermentation mainly conducted by Lp. 2211. Metabolic modeling revealed potential cross-feeding between W. paramesenteroides, while molecular docking dynamic simulation LAB tannases proteinases involved in chemical affinity gallocatechin trypsin inhibitors. better soaking, germination cooking enhancing properties: it free amino acids 50% releasing glutamine, glutamic acid, arginine, leucine lysine modified TPC increasing gallic decreasing caffeic, ferulic vanillic quercetin-3-glucoside. The combination experimental data may help us understand processes design products desirable features.

Language: Английский

Citations

0
Metagenomic Insights into Enhancing Protein Content and Digestibility in Jack Bean (Canavalia ensiformis) Tempeh: Unraveling Microbial Dynamics During Fermentation DOI Creative Commons
Vira Putri Yarlina,

Mohammad Djali,

Robi Andoyo

et al.

Applied Food Research, Journal Year: 2024, Volume and Issue: 4(2), P. 100588 - 100588

Published: Nov. 8, 2024

Language: Английский

Citations

0
The protective effect of freezing temperatures on different lactic acid bacteria and its mechanism DOI Creative Commons
Pin Chen, Wen Tan, Huan Cheng

et al.

LWT, Journal Year: 2024, Volume and Issue: unknown, P. 117226 - 117226

Published: Dec. 1, 2024

Language: Английский

Citations

0