Ultrasensitive Lateral Flow Immunoassay of Fluoroquinolone Antibiotic Gatifloxacin Using Au@Ag Nanoparticles as a Signal-Enhancing Label DOI Creative Commons
Olga D. Hendrickson, Nadezhda A. Byzova, Vasily G. Panferov

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 598 - 598

Published: Dec. 6, 2024

Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, method of ultrasensitive immunochromatographic detection GAT was developed for first time. An indirect format lateral flow immunoassay (LFIA) performed. GAT-specific monoclonal antibodies and labeled anti-species were used in LFIA. Bimetallic core@shell Au@Ag nanoparticles (Au@Ag NPs) synthesized as new label. Peroxidase-mimic properties NPs allowed catalytic enhancement signal on test strips, increasing assay sensitivity. A mechanism NPs-mediated catalysis deduced. Signal amplification achieved through oxidative etching by hydrogen peroxide. This resulted formation gold Ag+ ions, which catalyzed oxidation peroxidase substrate. Such “chemical enhancement” reaching instrumental limit (LOD, calculated Three Sigma approach) cutoff 0.8 20 pg/mL, respectively. The enhanced procedure can be completed 21 min. LFIA tested raw meat samples, recoveries from 78.1–114.8%. recommended promising instrument sensitive various toxicants.

Language: Английский

Ultrasensitive Lateral Flow Immunoassay of Fluoroquinolone Antibiotic Gatifloxacin Using Au@Ag Nanoparticles as a Signal-Enhancing Label DOI Creative Commons
Olga D. Hendrickson, Nadezhda A. Byzova, Vasily G. Panferov

et al.

Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 598 - 598

Published: Dec. 6, 2024

Gatifloxacin (GAT), an antibiotic belonging to the fluoroquinolone (FQ) class, is a toxicant that may contaminate food products. In this study, method of ultrasensitive immunochromatographic detection GAT was developed for first time. An indirect format lateral flow immunoassay (LFIA) performed. GAT-specific monoclonal antibodies and labeled anti-species were used in LFIA. Bimetallic core@shell Au@Ag nanoparticles (Au@Ag NPs) synthesized as new label. Peroxidase-mimic properties NPs allowed catalytic enhancement signal on test strips, increasing assay sensitivity. A mechanism NPs-mediated catalysis deduced. Signal amplification achieved through oxidative etching by hydrogen peroxide. This resulted formation gold Ag+ ions, which catalyzed oxidation peroxidase substrate. Such “chemical enhancement” reaching instrumental limit (LOD, calculated Three Sigma approach) cutoff 0.8 20 pg/mL, respectively. The enhanced procedure can be completed 21 min. LFIA tested raw meat samples, recoveries from 78.1–114.8%. recommended promising instrument sensitive various toxicants.

Language: Английский

Citations

1