Screening and Genomic Profiling of Antimicrobial Bacteria Sourced from Poultry Slaughterhouse Effluents: Bacteriocin Production and Safety Evaluation DOI Open Access

Nuria Peña,

Irene Lafuente,

Ester Sevillano

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1564 - 1564

Published: Dec. 2, 2024

Background/Objectives: Antimicrobial-resistant (AMR) pathogens represent a serious threat to public health, particularly in food production systems where antibiotic use remains widespread. As result, alternative antimicrobial treatments antibiotics are essential for effectively managing bacterial infections. This study aimed identify and characterize novel peptides produced by bacteria, known as bacteriocins, well recognize safe bacteriocin-producing strains, sourced from poultry slaughterhouse effluents. Methods: A total of 864 isolates were collected across eight stages slaughter line screened activity against Gram-positive Gram-negative indicator strains. Whole-genome sequencing (WGS) was performed on 12 selected including Enterococcus faecium (6 isolates), Lactococcus lactis (1 isolate), garvieae isolate) Escherichia coli (4 isolates). The presence bacteriocin gene clusters (BGC), resistance genes (ARG), virulence factors (VF) analyzed. further evaluated using vitro cell-free protein synthesis (IV-CFPS). Results: WGS revealed multiple BGCs, class IId bacteriocin, lactococcin P1A (LcnP1A), L. SWD9. LcnP1A showed various Listeria monocytogenes. While most bacteriocin-encoding strains harbored ARGs VFs, E. SWG6 notable its absence minimal highlighting potential probiotic. Conclusions: These findings underscore the importance discovering bacteriocins safer producing address chain. Further research would validate efficacy both isolate application processed animal systems.

Language: Английский

Gastronomic Treasures of Southern Italy: The Role of Wooden Equipment and Heritage Cheeses DOI
Gabriele Busetta, Luca Settanni, Raimondo Gaglio

et al.

International Journal of Gastronomy and Food Science, Journal Year: 2025, Volume and Issue: unknown, P. 101179 - 101179

Published: April 1, 2025

Language: Английский

Citations

0
Screening and Genomic Profiling of Antimicrobial Bacteria Sourced from Poultry Slaughterhouse Effluents: Bacteriocin Production and Safety Evaluation DOI Open Access

Nuria Peña,

Irene Lafuente,

Ester Sevillano

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1564 - 1564

Published: Dec. 2, 2024

Background/Objectives: Antimicrobial-resistant (AMR) pathogens represent a serious threat to public health, particularly in food production systems where antibiotic use remains widespread. As result, alternative antimicrobial treatments antibiotics are essential for effectively managing bacterial infections. This study aimed identify and characterize novel peptides produced by bacteria, known as bacteriocins, well recognize safe bacteriocin-producing strains, sourced from poultry slaughterhouse effluents. Methods: A total of 864 isolates were collected across eight stages slaughter line screened activity against Gram-positive Gram-negative indicator strains. Whole-genome sequencing (WGS) was performed on 12 selected including Enterococcus faecium (6 isolates), Lactococcus lactis (1 isolate), garvieae isolate) Escherichia coli (4 isolates). The presence bacteriocin gene clusters (BGC), resistance genes (ARG), virulence factors (VF) analyzed. further evaluated using vitro cell-free protein synthesis (IV-CFPS). Results: WGS revealed multiple BGCs, class IId bacteriocin, lactococcin P1A (LcnP1A), L. SWD9. LcnP1A showed various Listeria monocytogenes. While most bacteriocin-encoding strains harbored ARGs VFs, E. SWG6 notable its absence minimal highlighting potential probiotic. Conclusions: These findings underscore the importance discovering bacteriocins safer producing address chain. Further research would validate efficacy both isolate application processed animal systems.

Language: Английский

Citations

0