Harnessing the Potential of Spider Silk Proteins for Biomedical Applications: from Native Silk Fibers to Designed Bioactive Materials DOI Open Access
Shiyu Fang, Yue Li, Songzi Kou

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Spider silk has only been used anecdotally throughout human history, owing to limited supply from natural sources and recalcitrance toward biochemical functionalization. Recent advances in materials synthetic biology have ameliorated the challenges of mass‐producing spider thus paved way for reengineering redesigning these real‐world applications. Here, an update is presented on development biomaterials comprising proteins within past five years respective chemical genetic approaches behind developments. Potential applications are further highlighted areas such as 3‐dimension (3D) cell culturing, drug delivery, theranostics, wound healing, tissue engineering, anti‐infection, so on. By providing some glimpses into latest innovations centered around proteins, well facing their biomedical applications, it hoped that this will inspire more translational studies impact.

Language: Английский

Silk Protein Gene Engineering and Its Applications: Recent Advances in Biomedicine Driven by Molecular Biotechnology DOI Creative Commons

Xingxiang Ji,

Yanyan Li, Jingsheng Wang

et al.

Drug Design Development and Therapy, Journal Year: 2025, Volume and Issue: Volume 19, P. 599 - 626

Published: Jan. 1, 2025

Silk protein, as a natural polymer material with unique structures and properties, exhibits tremendous potential in the biomedical field. Given limited production restricted properties of silk proteins, molecular biotechnology has been extensively applied protein genetic engineering to produce novel proteins specific properties. This review outlines roles major model organisms, such silkworms spiders, production, provides detailed introduction applications gene editing technologies (eg, CRISPR-Cas9), transgenic expression technologies, synthetic biology techniques engineering. By analyzing factors influencing expression, this further elaborates on innovative drug delivery systems, tissue regenerative medicine skin, bone, cartilage, vascular repair), well antibacterial immune strategies. Notably, modified expressed by demonstrate significant advantages enhancing bioavailability promoting cell proliferation differentiation. In conclusion, engineering, through continuous innovations biotechnology, provided an effective pathway for high-performance materials. The extensive these field have not only expanded functionality but also offered new approaches address medical challenges. future, development will rely interdisciplinary integration promote in-depth research expansion industrial proteins.

Language: Английский

Citations

0
Chitosan/acrylamide/MXene hydrogel sensor with high mechanical properties, long-term sensing stability, rapid gelation via Fe2+ DOI

Jie Lou,

Jian Zheng, Zhijia Zhu

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 313, P. 144257 - 144257

Published: May 15, 2025

Language: Английский

Citations

0
Advances in biomimetic intelligent adhesives for wood composite applications DOI
Limin Wang,

Guanyan Li,

Qian Ma

et al.

Advanced Composites and Hybrid Materials, Journal Year: 2024, Volume and Issue: 7(5)

Published: Oct. 1, 2024

Language: Английский

Citations

2
Rapidly Forming Recombinant Miniature Spidroins Hydrogels Composed of Nanofibrils with Tunable Mechanical Properties for Bio 3D Printing and Biomimetic Cellular Scaffolds DOI Open Access
Cong Wang, Qi Zhang, Hanbai Wu

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

Abstract Recombinant spidroins offer numerous possibilities for creating new biomaterials. However, their polymorphic and prone to aggregation characteristics present challenges in both production practical application. Here, mutant recombinant are reported forming hydrogels rapidly controllably at 37 °C with visible light irradiation. In the phenylalanine residues (F) systematically substituted by tyrosine (Y) repeat motifs of (GGX), which contributes self‐assembly β ‐sheet further formation amyloid‐like nanofibrils. As expected, micellar/globular solution converts hydrogel composed nanofibrils network subsequently crosslinked di‐tyrosine. The conformation transformation process is verified spectroscopy, transmission electron microscopy (TEM), molecular dynamics simulation. Furthermore, spidroin used as bioink biomimetic cellular scaffolds according good biocompatibility, shear thinning properties, nanofibril structure. findings reveal structural mechanism expand applications biomedical engineering.

Language: Английский

Citations

1
Harnessing the Potential of Spider Silk Proteins for Biomedical Applications: from Native Silk Fibers to Designed Bioactive Materials DOI Open Access
Shiyu Fang, Yue Li, Songzi Kou

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Spider silk has only been used anecdotally throughout human history, owing to limited supply from natural sources and recalcitrance toward biochemical functionalization. Recent advances in materials synthetic biology have ameliorated the challenges of mass‐producing spider thus paved way for reengineering redesigning these real‐world applications. Here, an update is presented on development biomaterials comprising proteins within past five years respective chemical genetic approaches behind developments. Potential applications are further highlighted areas such as 3‐dimension (3D) cell culturing, drug delivery, theranostics, wound healing, tissue engineering, anti‐infection, so on. By providing some glimpses into latest innovations centered around proteins, well facing their biomedical applications, it hoped that this will inspire more translational studies impact.

Language: Английский

Citations

0