Interparticle Crosslinked Ion-responsive Microgels for 3D and 4D (Bio)printing Applications DOI Open Access
Vaibhav Pal, Deepak Gupta, Suihong Liu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Abstract Microgels offer unique advantages over bulk hydrogels due to their improved diffusion limits for oxygen and nutrients. Particularly, stimuli-responsive microgels with inherently bioactive self-supporting properties emerge as highly promising biomaterials. This study unveils the development of interparticle-crosslinked, self-supporting, ion-responsive tailored 3D 4D (bio)printing applications. A novel strategy was proposed develop that enabled interparticle crosslinking, eliminating need filler preserving essential microscale void spaces support cell migration vascularization. Additionally, these possessed unique, shrinking behavior primarily by Hofmeister effect, reversible upon removal stimulus. Two types microgels, spherical (µS) random-shaped (µR), were fabricated, µR exhibiting superior mechanical higher packing density. Fabricated microgel-based constructs supported angiogenesis tunable vessel size based on interstitial while demonstrating excellent shear-thinning self-healing high print fidelity. Various bioprinting techniques employed validated using including extrusion-based, embedded, intraembedded, aspiration-assisted bioprinting, facilitating biofabrication scalable constructs. Multi-material printing achieved combining non-responsive enabling programmable shape transformations exposure ionic solutions. Utilizing printing, complex, dynamic structures generated such coiling filaments, grippers, folding sheets, providing a foundation advanced tissue models devices regenerative medicine soft robotics, respectively.

Language: Английский

Insights Into Cold Atmospheric Plasma Triggered Self‐Assembly of Keratin Protein DOI Open Access
Priya Bhatt, Kamatchi Sankaranarayanan

Plasma Processes and Polymers, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

ABSTRACT Cold Atmospheric Plasma (CAP) is a novel method for biomaterial fabrication, inducing physicochemical changes in biomolecules. Keratin, an industrial waste product valued its biocompatibility and biodegradability, formed self‐assembled structures under CAP treatment, as revealed by SEM. Zeta analysis showed increased surface charge, while TGA XRD confirmed enhanced crystallinity with sharp‐edged nanocrystalline structures. Unique self‐assembly behaviors were observed un‐dialyzed dialyzed keratin. modified keratin's through reactive oxygen nitrogen species (RONS), physical without altering chemical structure or native conformation. This highlights CAP's potential to tailor keratin properties specific applications.

Language: Английский

Citations

1
Interparticle Crosslinked Ion-responsive Microgels for 3D and 4D (Bio)printing Applications DOI Open Access
Vaibhav Pal, Deepak Gupta, Suihong Liu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 2, 2025

Abstract Microgels offer unique advantages over bulk hydrogels due to their improved diffusion limits for oxygen and nutrients. Particularly, stimuli-responsive microgels with inherently bioactive self-supporting properties emerge as highly promising biomaterials. This study unveils the development of interparticle-crosslinked, self-supporting, ion-responsive tailored 3D 4D (bio)printing applications. A novel strategy was proposed develop that enabled interparticle crosslinking, eliminating need filler preserving essential microscale void spaces support cell migration vascularization. Additionally, these possessed unique, shrinking behavior primarily by Hofmeister effect, reversible upon removal stimulus. Two types microgels, spherical (µS) random-shaped (µR), were fabricated, µR exhibiting superior mechanical higher packing density. Fabricated microgel-based constructs supported angiogenesis tunable vessel size based on interstitial while demonstrating excellent shear-thinning self-healing high print fidelity. Various bioprinting techniques employed validated using including extrusion-based, embedded, intraembedded, aspiration-assisted bioprinting, facilitating biofabrication scalable constructs. Multi-material printing achieved combining non-responsive enabling programmable shape transformations exposure ionic solutions. Utilizing printing, complex, dynamic structures generated such coiling filaments, grippers, folding sheets, providing a foundation advanced tissue models devices regenerative medicine soft robotics, respectively.

Language: Английский

Citations

0