Bioactive Hydroxyapatite–Carboplatin–Quercetin Coatings for Enhanced Osteointegration and Antitumoral Protection in Hip Endoprostheses DOI Open Access

Gheorghe Iosub,

Dana-Ionela Tudorache,

Ionuț Marinel Iova

et al.

Coatings, Journal Year: 2025, Volume and Issue: 15(4), P. 489 - 489

Published: April 20, 2025

The recurrence of bone cancer poses severe complications, particularly after orthopedic surgery, necessitating advanced biomaterials with dual functionality. This study develops nanostructured coatings composed hydroxyapatite, carboplatin, and quercetin, designed to enhance regeneration while delivering localized therapy. These present a promising solution for hip endoprostheses, addressing osteointegration tumor prevention simultaneously. Hydroxyapatite was synthesized characterized using XRD, TEM, SAED, FTIR, SEM assess crystallinity, surface morphology, functional groups. were obtained by MAPLE. In vitro biocompatibility tests showed that HAp@CPT HAp@CPT/QUE supported osteoblast viability adhesion exhibiting selective cytotoxic effects on osteosarcoma cells. Griess assay indicated nitric oxide (NO) levels remained unchanged in hFOB osteoblasts, confirming neither coating induced inflammatory responses healthy contrast, MG63 cells exhibited significantly elevated NO (p < 0.05) response HAp@CPT/QUE, suggesting increased oxidative stress. MTT results 12% 28% reduction cell respectively. Phase-contrast microscopy further confirmed strong reduced attachment, surfaces. findings highlight the functionality hydroxyapatite–carboplatin–quercetin coatings, promoting exerting anticancer effects. Their bone-regenerative properties make them material endoprostheses oncological applications.

Language: Английский

Bioactive Hydroxyapatite–Carboplatin–Quercetin Coatings for Enhanced Osteointegration and Antitumoral Protection in Hip Endoprostheses DOI Open Access

Gheorghe Iosub,

Dana-Ionela Tudorache,

Ionuț Marinel Iova

et al.

Coatings, Journal Year: 2025, Volume and Issue: 15(4), P. 489 - 489

Published: April 20, 2025

The recurrence of bone cancer poses severe complications, particularly after orthopedic surgery, necessitating advanced biomaterials with dual functionality. This study develops nanostructured coatings composed hydroxyapatite, carboplatin, and quercetin, designed to enhance regeneration while delivering localized therapy. These present a promising solution for hip endoprostheses, addressing osteointegration tumor prevention simultaneously. Hydroxyapatite was synthesized characterized using XRD, TEM, SAED, FTIR, SEM assess crystallinity, surface morphology, functional groups. were obtained by MAPLE. In vitro biocompatibility tests showed that HAp@CPT HAp@CPT/QUE supported osteoblast viability adhesion exhibiting selective cytotoxic effects on osteosarcoma cells. Griess assay indicated nitric oxide (NO) levels remained unchanged in hFOB osteoblasts, confirming neither coating induced inflammatory responses healthy contrast, MG63 cells exhibited significantly elevated NO (p < 0.05) response HAp@CPT/QUE, suggesting increased oxidative stress. MTT results 12% 28% reduction cell respectively. Phase-contrast microscopy further confirmed strong reduced attachment, surfaces. findings highlight the functionality hydroxyapatite–carboplatin–quercetin coatings, promoting exerting anticancer effects. Their bone-regenerative properties make them material endoprostheses oncological applications.

Language: Английский

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