Gene, Journal Year: 2022, Volume and Issue: 851, P. 147011 - 147011
Published: Oct. 27, 2022
Language: Английский
International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(10), P. 3485 - 3485
Published: May 15, 2020
The potential of extracellular circulating microRNAs (miRNAs) as non-invasive biomarkers atrial fibrillation (AF) has been confirmed by a number recent studies. However, the current data for some miRNAs are controversial and inconsistent, probably due to pre-analytical methodological differences. In this work, we attempted fulfill basic requirements provided miRNA studies application paroxysmal (PAF) research. We used quantitative PCR (qPCR) determine relative plasma levels expressed in heart or associated with remodeling reported altered plasma/serum AF: miR-146a-5p, miR-150-5p, miR-19a-3p, miR-21-5p, miR-29b-3p, miR-320a-3p, miR-328-3p, miR-375-3p, miR-409-3p. First, cohort 90 adult outpatient clinic patients, found that level miR-320a-3p was elevated PAF patients compared healthy controls hypertensive without AF. further analyzed impact medication therapies on receiving angiotensin-converting-enzyme inhibitors (ACEI) therapy. Additionally, miR-146a-5p positively correlated CHA2DS2-Vasc score were subjects ≥ 2. Our results indicate that, amongst miRNAs, may be considered biomarker, leading speculation whether is marker platelet state change ACEI
Language: Английский
Citations
30
Cell Stress and Chaperones, Journal Year: 2020, Volume and Issue: 26(1), P. 77 - 90
Published: Sept. 7, 2020
Cardiovascular diseases are the leading cause of death globally, among which acute myocardial infarction (AMI) frequently occurs in heart and proceeds from myocardium ischemia endoplasmic reticulum (ER) stress-induced cell death. Numerous studies on miRNAs indicated their potential as diagnostic biomarkers treatment targets for diseases. Our study investigated role miR-17-5p its regulatory mechanisms during AMI. Echocardiography, MTT, flow cytometry assay, evaluation caspase-3 lactate dehydrogenase (LDH) activity were conducted to assess viability, apoptosis an MI/R mice model, H2O2-induced H9c2 hypoxia respectively. The expression levels ER stress response-related detected using qRT-PCR, IHC, western blotting assays. binding site Tsg101 mRNA was determined by bioinformatic prediction luciferase reporter assay. notably elevated models, accompanied enhanced apoptosis. Inhibition led decreased related response could be counteracted knockdown (tumor susceptibility gene 101). Transfection with mimics downregulated cells. Luciferase assay demonstrated between Tsg101. Moreover, 4-PBA, inhibitor response, abolished shTsg101 hypoxic findings pro-apoptotic MI/R, disclosed specific mechanism miR-17-5p/Tsg101 axis injury cardiomyocytes apoptosis, presented a promising biomarker target therapy
Language: Английский
Citations
29
Biomolecules, Journal Year: 2021, Volume and Issue: 11(6), P. 881 - 881
Published: June 14, 2021
Despite significant strides to mitigate the complications of acute coronary syndrome (ACS), this clinical entity still represents a major global health burden. It has so far been well-established that most plaques leading ACS are not result gradual narrowing vessel lumen, but rather sudden disruption vulnerable atherosclerotic plaques. As developed imaging modalities for plaque detection invasive, multiple biomarkers were proposed identify their presence. Owing pivotal role lipids and inflammation in pathophysiology atherosclerosis, originated from one those processes, whereas recent advancements molecular sciences shed light on use microRNAs. Yet, at present there no clinically implemented or any other method matter could non-invasively, yet reliably, diagnose plaque. Hence, review we summarized available knowledge regarding instability, current evidence potential associated with destabilization finally, discussed if search day bring us non-invasive, cost-effective, valid way diagnosing vulnerable, rupture-prone artery
Language: Английский
Citations
26
Diagnostics, Journal Year: 2021, Volume and Issue: 11(2), P. 318 - 318
Published: Feb. 16, 2021
The mechanisms that regulate the systemic extent of atherosclerosis are not fully understood. We investigated whether expression circulating miRNAs is associated with stable to a single territory or multiple territories (polyvascular) and severity in each territory. Ninety-four participants were prospectively recruited divided into five age- sex-matched groups: presenting no atherosclerosis, isolated coronary lower extremity carotid coronary, extremity, territories. six distinct biological roles was assessed. miR-27b miR-146 differed across groups (
Language: Английский
Citations
24
Frontiers in Cardiovascular Medicine, Journal Year: 2022, Volume and Issue: 8
Published: Jan. 17, 2022
Myocardial infarction (MI) is the most frequent end-point of cardiovascular pathology, leading to higher mortality worldwide. Due particularity heart tissue, patients who experience ischemic heart, still suffered irreversible damage even if vascular reflow by treatment, and severe ones can lead failure or death. In recent years, several studies have shown that microRNAs (miRNAs), playing a regulatory role in damaged hearts, bring light for alleviate MI. this review, we summarized effect miRNAs on MI with some mechanisms, such as apoptosis, autophagy, proliferation, inflammatory; regulation cardiac structural changes after MI, including angiogenesis, myocardial remodeling, fibrosis; application stem cell therapy clinical diagnosis; other non-coding RNAs related during past 5 years.
Language: Английский
Citations
17
CARDIOVASCULAR THERAPY AND PREVENTION, Journal Year: 2025, Volume and Issue: 23(12), P. 4225 - 4225
Published: Feb. 13, 2025
The literature review is devoted to circulating microRNA (small noncoding ribonucleic acid molecules) of blood plasma and serum, potential their use as new biomarkers coronary artery disease (CAD) at various stages. study results demonstrate different levels serum expression in patients with without CAD. In this regard, determination level for minimally invasive diagnosis CAD seems promising, well further prognosis progression. A list microRNAs has been compiled.
Language: Английский
Citations
0
Seminars in Thrombosis and Hemostasis, Journal Year: 2025, Volume and Issue: unknown
Published: March 12, 2025
Abstract Coronary artery disease (CAD) is a leading cause of morbidity and mortality worldwide, with platelet reactivity playing central role in its pathogenesis. Recent research has identified microRNAs (miRNAs; miRs) as potential biomarkers for CAD, due to their ability regulate function reactivity. This review focuses on four key miRNAs—miR-223, miR-126, miR-21, miR-150—known influence implications CAD. miR-223, which highly expressed platelets, shown associations CAD myocardial infarction, while miR-126 been linked thrombus formation vascular health. Additionally, miR-21 miR-150 have also emerged important players, roles cardiovascular outcomes. However, despite potential, the use miRNAs clinical faces several challenges, including variability reported results across studies. These inconsistencies often arise from differences sample material, preanalytical conditions, normalization strategies. Furthermore, antiplatelet therapy miRNA expression adds another layer complexity, making it difficult determine whether observed changes levels are states or therapeutic interventions. therefore highlights need standardization enhance reliability findings. By addressing these methodological could become powerful tools personalized medicine, aiding development tailored strategies patients ultimately improving
Language: Английский
Citations
0
Clinica Chimica Acta, Journal Year: 2025, Volume and Issue: unknown, P. 120304 - 120304
Published: April 1, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(6), P. 3153 - 3153
Published: March 19, 2021
Type 2 diabetes mellitus (T2DM) and its complications pose a serious threat to the life health of patients around world. The most dangerous this disease are vascular complications. Microvascular T2DM include retinopathy, nephropathy, neuropathy. In turn, macrovascular coronary artery disease, peripheral cerebrovascular disease. currently used diagnostic methods do not ensure detection at an early stage, they also predict risk developing specific MicroRNAs (miRNAs) small, endogenous, noncoding molecules that involved in key processes, such as cell proliferation, differentiation, apoptosis. Recent research has assigned them important role potential biomarkers for detecting related diabetes. We suggest utilizing miRNAs can be routine approach diagnosis prognosis diseases may enable development better therapeutic approaches. paper, we conduct review latest reports demonstrating usefulness T2DM.
Language: Английский
Citations
23
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: May 24, 2024
Abstract Coronary artery disease (CAD) imposes a significant economic burden in developing countries like India. Timely diagnosis and treatment should be prioritized to mitigate the disease. Current diagnostic tools being invasive less specific raise need develop more reliable molecular biomarkers. MicroRNAs (miRNAs) are an emerging class of molecules that can serve as potential source non-invasive biomarkers for CAD. The objective this study was determine circulatory miRNAs In study, we have reported two microRNAs, miR-128-3p miR-195-5p serum CAD patients Indian Population. A total 124 subjects were recruited which included 89 angiographically proven 35 control subjects. Our results show decrease levels while there no changes miR-195-5p. Further bioinformatics analysis revealed role cholesterol homeostasis. Altered homeostasis due accumulation macrophages is driving force behind formation foam cells turn accelerates progression Here, shown increases by decreasing efflux in-vitro.
Language: Английский
Citations
3