Homeology of sex chromosomes in Amazonian Harttia armored catfishes supports the X-fission hypothesis for the X1X2Y sex chromosome system origin DOI Creative Commons
Francisco de Menezes Cavalcante Sassi, Alexandr Sember, Geize Aparecida Deon

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 21, 2023

Abstract The Neotropical monophyletic catfish genus Harttia represents an excellent model to study karyotype and sex chromosome evolution in teleosts. Its species split into three phylogenetic clades distributed along the Brazilian territory they differ widely traits, including presence of standard or multiple systems some members. Here, we investigate chromosomal rearrangements associated synteny blocks involved origin a X 1 2 Y system present out six sampled Amazonian-clade species. Using 5S 18S ribosomal DNA fluorescence situ hybridization whole painting with probes corresponding chromosomes from H. punctata , confirm previous assumptions that duriventris villasboas represent same linkage groups which also form putative XY rondoni . shared homeology between suggests might have originated once common ancestor these closely related A joint arrangement mapped early diverging different may formed through fission rather than previously proposed Y-autosome fusion.

Language: Английский

Sex determination mechanisms and sex control approaches in aquaculture animals DOI
Xi‐Yin Li, Jie Mei, Chutian Ge

et al.

Science China Life Sciences, Journal Year: 2022, Volume and Issue: 65(6), P. 1091 - 1122

Published: May 16, 2022

Language: Английский

Citations

101
Why do sex chromosomes progressively lose recombination? DOI
Paul Jay, Daniel L. Jeffries, Fanny E. Hartmann

et al.

Trends in Genetics, Journal Year: 2024, Volume and Issue: 40(7), P. 564 - 579

Published: April 27, 2024

Language: Английский

Citations

13
Evolution and regulation of animal sex chromosomes DOI
Zexian Zhu, Lubna Younas, Qi Zhou

et al.

Nature Reviews Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: July 18, 2024

Language: Английский

Citations

11
Comparative analysis of amphibian genomes: An emerging resource for basic and applied research DOI Creative Commons
Tiffany A. Kosch, Andrew J. Crawford, Rachel Lockridge Mueller

et al.

Molecular Ecology Resources, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 4, 2024

Abstract Amphibians are the most threatened group of vertebrates and in dire need conservation intervention to ensure their continued survival. They exhibit unique features including a high diversity reproductive strategies, permeable specialized skin capable producing toxins antimicrobial compounds, multiple genetic mechanisms sex determination some lineages, ability regenerate limbs organs. Although genomic approaches would shed light on these traits aid conservation, sequencing assembly amphibian genomes has lagged behind other taxa due comparatively large genome sizes. Fortunately, development long‐read technologies initiatives led recent burst new assemblies. growing, field genomics suffers from lack annotation resources, tools for working with challenging high‐quality assemblies clades amphibians. Here, we analyse 51 publicly available evaluate usefulness functional research. We report considerable variation quality completeness highest transposable element repeat contents any vertebrate. Additionally, detected an association between content climatic variables. Our analysis provides evidence conserved synteny despite long divergence times this group, but also highlight inconsistencies chromosome naming orientation across discuss gaps phylogeny suggest key targets future endeavours. Finally, propose increased investment research promote conservation.

Language: Английский

Citations

8
Why Do Some Vertebrates Have Microchromosomes? DOI Creative Commons
Kornsorn Srikulnath, Syed Farhan Ahmad, Worapong Singchat

et al.

Cells, Journal Year: 2021, Volume and Issue: 10(9), P. 2182 - 2182

Published: Aug. 24, 2021

With more than 70,000 living species, vertebrates have a huge impact on the field of biology and research, including karyotype evolution. One prominent aspect many vertebrate karyotypes is enigmatic occurrence tiny often cytogenetically indistinguishable microchromosomes, which possess distinctive features compared to macrochromosomes. Why certain species carry these microchromosomes in some lineages while others do not, how they evolve remain open questions. New studies shown that exhibit unique characteristics genome structure organization, such as high gene densities, low heterochromatin levels, rates recombination. Our review focuses recent concepts expand current knowledge dynamic nature evolution vertebrates, raising important questions regarding evolutionary origins ramifications microchromosomes. We introduce basic karyotypic clarify size, shape, morphology macro- report their distribution across different lineages. Finally, we characterize mechanisms forces underlying origin

Language: Английский

Citations

40
Analysis in Proceratophrys boiei genome illuminates the satellite DNA content in a frog from the Brazilian Atlantic forest DOI Creative Commons
Marcelo João da Silva, Thiago Gazoni, Célio F. B. Haddad

et al.

Frontiers in Genetics, Journal Year: 2023, Volume and Issue: 14

Published: March 29, 2023

Satellite DNAs (satDNAs) are one of the most abundant elements in genomes. Characterized as tandemly organized sequences that can be amplified into multiple copies, mainly heterochromatic regions. The frog P. boiei (2n = 22, ZZ♂/ZW♀) is found Brazilian Atlantic forest and has an atypical pattern heterochromatin distribution when compared to other anuran amphibians, with large pericentromeric blocks on all chromosomes. In addition, females Proceratophrys have a metacentric sex chromosome W showing chromosomal extension. this work, we performed high-throughput genomic, bioinformatic, cytogenetic analyses characterize satellite DNA content (satellitome) boiei, due high amount C-positive highly chromosome. After analyses, it remarkable satellitome composed number satDNA families (226), making species highest satellites described so far. Consistent observation centromeric blocks, genome enriched copy repetitive DNAs, total abundance comprising 16.87% genome. We successfully mapped via Fluorescence situ hybridization two repeats genome, PboSat01-176 PboSat02-192, highlighting presence certain satDNAs strategic regions (e.g., centromere region), which leads their participation crucial processes for genomic organization maintenance. Our study reveals great diversity driving species. characterization approaches regarding allowed confirmation some insights from biology possible relationship evolution chromosomes, especially including data were not available.

Language: Английский

Citations

12
HerpSexDet: the herpetological database of sex determination and sex reversal DOI Creative Commons
Edina Nemesházi, Veronika Bókony

Scientific Data, Journal Year: 2023, Volume and Issue: 10(1)

Published: June 13, 2023

Abstract Wildlife exhibits various sex-determination systems where sex chromosomes and environmental temperatures may both contribute to individual sexual development. The causes consequences of this variability are important questions for evolutionary ecology, especially in light ongoing change. Amphibians reptiles emerging as a key group studying these questions, with new data accumulating acceleratingly. We collected empirical from earlier databases, reviews primary literature create the most up-to-date database on herpetological determination. named our HerpSexDet, which currently features genetic temperature-dependent determination well reports reversal total 192 amphibian 697 reptile species. This dataset, we will regularly update future, facilitates interspecific comparative studies evolution its species-specific traits such life history conservation status, also help guiding future research by identifying species or higher taxa that potentially enlightening study environmentally driven reversal.

Language: Английский

Citations

10
Genetic differentiation in the MAT-proximal region is not sufficient for suppressing recombination in Podospora anserina DOI Creative Commons
Pierre Grognet, Robert Debuchy, Tatiana Giraud

et al.

G3 Genes Genomes Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Recombination is advantageous over the long-term, as it allows efficient selection and purging deleterious mutations. Nevertheless, recombination suppression has repeatedly evolved in sex mating-type chromosomes. The evolutionary causes for proximal mechanisms preventing crossing overs are poorly understood. Several hypotheses have recently been suggested based on theoretical models, particular that divergence could accumulate neutrally around a sex-determining region reduce rates, self-reinforcing process foster progressive extension of suppression. We used ascomycete fungus Podospora anserina investigating these questions: 0.8 Mbp its locus non-recombining, despite being collinear between two mating types. This mostly selfing, resulting highly homozygous individuals, except non-recombining displays differentiation Here, we test hypothesis sequence alone responsible cessation. replaced mat- idiomorph by mat+ idiomorph, to obtain strain sexually compatible with reference isogenic this MAT-proximal region. Crosses showed was still suppressed mutant strains, indicating other than inversions or mere fungus. finding suggests selective likely acted suppressing recombination, spread epigenetic marks, neutral model nucleotide does not seem hold P. anserina.

Language: Английский

Citations

0
Chromosome homologies and polymorphisms in a Neotropical species complex of frogs revealed by the U2 snRNA gene DOI
Lucas Henrique Bonfim Souza,

Bruno Cansanção Silva,

Jennifer Nunes Pompeo

et al.

Genome, Journal Year: 2025, Volume and Issue: 68, P. 1 - 11

Published: Jan. 1, 2025

The Physalaemus cuvieri–Physalaemus ephippifer species complex is a Neotropical frog group that encompasses seven well-supported major clades. Although very similar morphologically, the five lineages previously karyotyped show notorious cytogenetic signatures. There also evidence of ancient secondary contact between P. ephippifer, which has heteromorphic sex chromosomes, and lineage known as L1B, lacks chromosome heteromorphism. Here, to aid comparative analysis within this complex, we mapped U2 small nuclear RNA (snRNA) gene using fluorescent in situ hybridization (FISH). All samples presented snRNA cluster terminally short arm 6. Additional FISH signals were revealed, particularly one with noted polymorphism nucleolar organizer regions. Moreover, additional site contributed for since Z harbors signal, absent W chromosome. In 9—which homologous chromosomes ephippifer—is polymorphic did derived from these lineages. Finally, nucleotide sequence revealed some truncated sequences, suggesting presence pseudogenes frogs.

Language: Английский

Citations

0
A Chromosome-level genome assembly of the American bullfrog (Aquarana catesbeiana) DOI Creative Commons
Kai Zhang, Yuxuan Zhang,

Ye Tian

et al.

Scientific Data, Journal Year: 2025, Volume and Issue: 12(1)

Published: March 10, 2025

The American bullfrog (Aquarana catesbeiana) is both an economically important aquaculture species and a globally distributed invasive organism with high environmental adaptability. In this study, we present high-quality chromosome-level genome assembly for the species, comprising 13 chromosomes total length of 6.32 Gb scaffold N50 691.8 Mb. Genome completeness was evaluated at 95.5% using BUSCO 99.9% Merqury. Repetitive sequences accounted 79.51% genome. Through combination RNA-seq, Ab initio homology-based gene prediction, identified 32,382 protein-coding genes, 98.96% these genes functionally annotated. This provides resource future studies on evolution, functional genomics molecular breeding bullfrog.

Language: Английский

Citations

0