Cited by Virus-Induced galactinol-sucrose galactosyltransferase 2 Silencing Delays Tomato Fruit Ripening

Metabolomics and transcriptomics analysis revealed the response mechanism of alfalfa to combined cold and saline‐alkali stress DOI

Lei Liu,

Liang Si,

Lishuang Zhang

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(4), P. 1900 - 1919

Published: June 29, 2024

SUMMARY Cold and saline‐alkali stress are frequently encountered by plants, they often occur simultaneously in soils at mid to high latitudes, constraining forage crop distribution production. However, the mechanisms which crops respond combination of cold remain unknown. Alfalfa ( Medicago sativa L.) is one most essential grasses world. In this study, we analyzed complex response two alfalfa species (Zhaodong [ZD] Blue Moon [BM]) combined using multi‐omics. The results revealed that ZD had a greater ability tolerate than BM. tricarboxylic acid cycles varieties responded positively stress, with accumulating more sugars, amino acids, jasmonic acid. gene expression flavonoid content biosynthesis pathway were significantly different between varieties. Weighted co‐expression network analysis based on RNA‐Seq data suggested MsMYB12 may regulating pathway. can directly bind promoter MsFLS13 promote its expression. Moreover, overexpression enhance flavonol accumulation antioxidant capacity, improve tolerance. These findings provide new insights into improving resistance showing flavonoids for plant stresses, theoretical guidance future breeding programs.

Language: Английский

Citations

Significance of Raffinose Family Oligosaccharides (RFOs) metabolism in plants DOI

Huan Liu, Fan Wang, Baohui Liu

et al.

Advanced Biotechnology, Journal Year: 2024, Volume and Issue: 2(2)

Published: March 19, 2024

Raffinose Family Oligosaccharides (RFOs) are a kind of polysaccharide containing D-galactose, and they widely exist in higher plants. Synthesis RFOs begins with galactinol synthase (GolS; EC 2.4.1.123) to convert myo-inositol into galactinol. The subsequent formation raffinose stachyose catalyzed by (RS; 2.4.1.82) (STS; 2.4.1.67) using sucrose as substrate, respectively. hydrolysis is finished α-galactosidase (α-Gal; 3.2.1.22) produce galactose. Importance metabolism have been summarized, e.g. In translocating plants, the phloem loading unloading reported mediating plant development process. Interference function synthesis or enzymes caused growth defect. addition, involved biotic abiotic stresses was discussed this review. Overall, literature summarizes our current understanding points out knowledge gaps that need be filled future.

Language: Английский

Citations

Expression profiling of galactinol synthase genes in Coffea arabica L. in response to Hemileia vastatrix infection DOI

Tiago Benedito dos Santos, Diliane Harumi Yaguinuma, Viviane Yumi Baba

et al.

Journal of Plant Diseases and Protection, Journal Year: 2025, Volume and Issue: 132(2)

Published: Feb. 27, 2025

Language: Английский

Citations

Integrative analysis of transcriptome and metabolome reveal molecular mechanism of tolerance to salt stress in rice DOI

Rui Deng,

Yao Li, Naijie Feng

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 15, 2025

Salt stress is considered to be one of the major abiotic stresses influencing rice growth and productivity. To improve crop productivity in saline soils, it essential choose a suitable variety for mitigating salt gain deep understanding underlying mechanisms. The current study explored tolerance mechanism wild 'HD96-1 (salt resistive)' conventional 'IR29 sensitive)' by evaluating morph-physiological, transcriptomic, metabolomic approaches. Physiological data indicated that HD96-1 had higher chlorophyll content, photosynthetic efficiency, more stable Na+/K+, less H₂O₂, lower electrolyte leakage under compared with IR29. Transcriptomic showed expression NHXs IR29 was significantly down-regulated stress, leading large accumulation Na⁺ cytoplasm, CHLH, PORA, PORB down-regulated, inhibiting synthesis. maintained balance K⁺ increasing NHX4, there no significant change genes related synthesis, which made resistant than In addition, inhibited excessive synthesis hydrogen peroxide (H₂O₂) alleviated oxidative damage down-regulating ACX4 stress. promoted isoleucine up-regulating branched-chain amino acid aminotransferase 2 4 might promote raffinose stachyose gene galactitol synthase 2, which, turn, osmotic pressure relieved We also found inhibition photosynthesis light-harvesting chromophore protein complex (LHCH II)-related reducing glucose metabolites, respectively. enhances regulating C2H2 bHLH153 transcription factors. Under ionic efficiency NHX4 overaccumulation respectively, mitigated promoting isoleucine, thereby enhancing adaptability genes, adaptation

Language: Английский

Citations

The Combination of Physiological and Transcriptomic Approaches Reveals New Insights into the Molecular Mechanisms of Leymus chinensis Growth Under Different Shading Intensities DOI

Xinru Li,

Qianqian Yu,

Zizhen Yao

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2730 - 2730

Published: March 18, 2025

Leymus chinensis is a grass species in the family Triticeae that found Eurasian grassland region and known for its outstanding ecological advantages economic value. However, increasing adoption of photovoltaic agriculture has modified light environment grass, markedly inhibiting photosynthesis, growth, yield. This study used physiological transcriptomic analyses to investigate complex response mechanisms two L. genotypes (Zhongke No. 3 [Lc3] Zhongke 5 [Lc5]) under shading stress. Growth phenotype analysis revealed superior growth performance Lc3 stress, evidenced by enhanced plant height photosynthetic parameters. Additionally, differentially expressed genes (DEGs) were predominantly enriched starch sucrose metabolism glycolysis/gluconeogenesis pathways, which most consistently both genotypes. flavonoid biosynthesis galactose pathways more Lc3. Weighted gene co-expression network identified LcGolS2 gene, encodes galactinol synthase, as potential hub resistance shade stress comparisons across different cultivars treatments. The use qRT-PCR further validated involved these suggesting they may play critical roles regulating development conditions. These findings provide new insights into molecular underlying

Language: Английский

Citations

CsABF8 mediates drought-induced ABA signaling in the regulation of raffinose biosynthesis in Camellia sinensis leaves DOI

Dongqiao Zheng,

Maoyin Fu,

Chenyi Sun

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143521 - 143521

Published: April 28, 2025

Language: Английский

Citations

Assessing drought stress response in low-gliadin wheat developed via RNAi and CRISPR/Cas DOI

Latifa Chaouachi, María H. Guzmán-López, Chahine Karmous

et al.

Plant Stress, Journal Year: 2025, Volume and Issue: unknown, P. 100906 - 100906

Published: May 1, 2025

Language: Английский

Citations

Transcriptomic and metabolomic profiling provide insight into the role of sugars and hormones in leaf senescence of Pinellia ternata DOI

Jialei Chen, Jialu Wang, Li Liu

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(5)

Published: April 22, 2024

Language: Английский

Citations

Differential Gene Expression in Contrasting Common Bean Cultivars for Drought Tolerance during an Extended Dry Period DOI

Talita Pijus Ponce,

Michely da Silva Bugança,

Victória Stern da Silva

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(7), P. 935 - 935

Published: July 17, 2024

Common beans (Phaseolus vulgaris L.), besides being an important source of nutrients such as iron, magnesium, and protein, are crucial for food security, especially in developing countries. bean cultivation areas commonly face production challenges due to drought occurrences, mainly during the reproductive period. Dry spells last approximately 20 days, enough time compromise production. Hence, it is understand genetic molecular mechanisms that confer tolerance improve common cultivars’ adaptation drought. Sixty six RNASeq libraries, generated from tolerant sensitive cultivars sourced R5 phenological stage at 0 days water deficit were sequenced, over 1.5 billion reads, aligned 62,524 transcripts originating a reference transcriptome, well 6673 obtained via de novo assembly. Differentially expressed functionally annotated, revealing variety genes associated with functions oxidoreductase transferase activity, biological processes related stress response signaling. The presence regulatory involved signaling cascades transcriptional control was also highlighted, example, LEA proteins dehydrins dehydration protection, transcription factors WRKY, MYB, NAC, which modulate plant deficit. Additionally, membrane protein ion uptake transport, identified, including aquaporins, RING-type E3 ubiquitin transferases, antioxidant enzymes GSTs CYPs, thioredoxins. This study highlights complexity scarcity, focusing on functional diversity their participation essential stress. identification cell protection offers promising prospects improvement aiming varieties more resistant These findings have potential drive sustainable agriculture, providing valuable insights ensure security context climate change.

Language: Английский

Citations

Analysis of the Rice Raffinose Synthase (OsRS) Gene Family and Haplotype Diversity DOI

Jinguo Zhang,

Dezhuang Meng,

Jianfeng Li

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9815 - 9815

Published: Sept. 11, 2024

Based on the genome information of rice (Nipponbare), this study screened and identified six raffinose synthase (RS) genes analyzed their physical chemical properties, phylogenetic relationship, conserved domains, promoter cis-acting elements, function genetic diversity gene-CDS-haplotype (gcHap). The results showed that these play key roles in abiotic stress response, such as

Language: Английский

Citations