Pan-Genome Identification and Expression Analysis of Lipoxygenase Genes in Cucumber

Agriculture, Journal Year: 2025, Volume and Issue: 15(3), P. 245 - 245

Published: Jan. 23, 2025

Lipoxygenase (LOX) is a dioxygenase that contains non-heme iron and plays crucial role in regulating plant growth development, signal transduction, responses to both biotic abiotic stresses. In this study, we identified 24 CsLOXs from the pan-genome of 12 cucumber (Cucumis sativus L.) accessions, with most CsLOX proteins exhibiting amino acid variations. To elucidate their functions, examined phylogenetic relationships, gene structures, conserved domains, promoter cis-elements, collinearity newly updated genome version 4.0 ‘Chinese Long 9930’. The results indicated can be categorized into three subfamilies: 9-LOX, Type I 13-LOX, II 13-LOX. Additionally, analysis revealed promoters contain various cis-elements related stress hormone responses. expression demonstrated tissue specificity, each expressed at least one tissue, six across all tissues. Furthermore, transcriptome data heat, cold, powdery mildew (PM), downy (DM), gray mold (GM) stresses, eight, four, four exhibited differential expression, respectively. Notably, CsLOX22 responded DM, GM Our provided reference for further exploring functions cucumber.

Language: Английский

A Genome-Wide Analysis of the Jasmonic Acid Biosynthesis Gene Families in Peanut Reveals Their Crucial Roles in Growth and Abiotic Stresses

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 7054 - 7054

Published: June 27, 2024

Abiotic stress is a limiting factor in peanut production. Peanut an important oil crop and cash China. yield vulnerable to abiotic due its seeds grown underground. Jasmonic acid (JA) essential for plant growth defense against adversity stresses. However, the regulation mechanism of jasmonic biosynthesis pathway on stresses are still limitedly understood. In this study, total 64 genes encoding key enzymes JA were identified classified into lipoxygenases (AhLOXs), alleno oxide synthases (AhAOSs), allene cyclases (AhAOCs), 12-oxo-phytodienoic reductases (AhOPRs) according gene structure, conserved motif, phylogenetic feature. A cis-regulatory element analysis indicated that some contained responsive hormone elements. addition proteins involved signaling, they also interacted with lipid response. Sixteen putative Ah-miRNAs from four families targeting 35 biosynthesis. tissue expression pattern revealed AhLOX2 was highest expressed leaf tissues, AhLOX32 shoot, root, nodule tissues. AhLOX16, AhOPR1, AhOPR3 up-regulated under drought stress. AhAOS3, AhAOC4 had elevated transcript levels response cold AhLOX5, AhAOC3, salt Our study could provide reference resistance peanut.

Language: Английский

Identification and Expression Analysis of Lipoxygenase Gene in Bitter Gourd (Momordica charantia)

Genes, Journal Year: 2024, Volume and Issue: 15(12), P. 1557 - 1557

Published: Nov. 29, 2024

Background: Lipoxygenases (LOXs) are key enzymes in the unsaturated fatty acid oxidation reaction pathway and play an important regulatory role synthesis of fruit aroma volatiles. Methods: LOX gene family members were identified whole genome database bitter gourd analyzed bioinformatically. An RT-qPCR was used to analyze expression differences different tissues. Monoterpenes determined by gas chromatography-mass spectrometry (GC-MS) technique. Results: A total 12 genome. The genes varied significantly among tissues roots, stems, leaves, flowers, fruits, seeds tendrils. 29 monoterpenes detected fruits five colors gourd, mainly containing six types alcohols, aldehydes, terpenes, ketones, esters alkynes, with highest relative content alcohols. Conclusions: present study provides a reference for further elucidation biological functions volatiles gourd.

Language: Английский